Abstract
Anthocyanins have attracted a great deal of attention due to their capability in protecting humans from chronic diseases. Production of natural anthocyanins from fresh plant material has many limitations. Therefore, the use of plant biotechnology for the production of anthocyanin has been put in focus without such restrictions. From the other hand, some crab apples can produce anthocyanins in their various organs including in vitro-grown calli. In this study, in order to investigate the effect of different concentrations of magnesium and nitrogen source for in vitro production of anthocyanin from callus cultures of a red-fleshed crab apple genotype, leaf explants from in vitro grown seedling was exploited to produce callus. After obtaining sufficient amount of callus, treatments were exerted in a completely randomized design with four replications for each treatment and four explants in each replication. With addition of magnesium to the medium, anthocyanin production increased up to 3 fold and the highest concentration of anthocyanins was observed in the highest magnesium concentration (40 mM). However, at this concentration, the growth rate of calli reduced. Changing the nitrogen source also affected the production of anthocyanins. The best nitrogen source for the production of anthocyanin was the combination of 10 mM ammonium and 18.8 mM nitrate as well as 5 mM ammonium and 25 mM nitrate.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Magnesium increased anthocyanin production up to threefold.
- Taxon
- Apple
Malus domesticat0 - Treatment
- addition of magnesium to the mediume0
- Outcome
- anthocyanin productione0
- Direction
- increasede0
- Value
- 3 folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With addition of magnesium to the medium, anthocyanin production increased up to 3 fold”
t0“Apple”
Claim · model-written summary
The highest magnesium concentration produced the highest anthocyanin concentration.
- Taxon
- AppleMalus domesticat0
- Treatment
- 40 mMe0
- Outcome
- concentration of anthocyaninse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest concentration of anthocyanins was observed in the highest magnesium concentration (40 mM).”
t0“Apple”
Claim · model-written summary
At the highest magnesium concentration, callus growth rate decreased.
- Taxon
- AppleMalus domesticat0
- Treatment
- 40 mMe0
- Outcome
- growth rate of callie0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest magnesium concentration (40 mM). However, at this concentration, the growth rate of calli reduced.”
t0“Apple”
Claim · model-written summary
Changing nitrogen source affected anthocyanin production.
- Taxon
- AppleMalus domesticat0
- Treatment
- nitrogen sourcee0
- Outcome
- production of anthocyaninse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changing the nitrogen source also affected the production of anthocyanins.”
t0“Apple”
Claim · model-written summary
Ten mM ammonium with 18.8 mM nitrate was among the best nitrogen sources for anthocyanin production.
- Taxon
- AppleMalus domesticat0
- Treatment
- 10 mM ammonium and 18.8 mM nitratee0
- Outcome
- production of anthocyanine0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best nitrogen source for the production of anthocyanin was the combination of 10 mM ammonium and 18.8 mM nitrate as well as 5 mM ammonium and 25 mM nitrate.”
t0“Apple”
Claim · model-written summary
Five mM ammonium with 25 mM nitrate was among the best nitrogen sources for anthocyanin production.
- Taxon
- AppleMalus domesticat0
- Treatment
- 5 mM ammonium and 25 mM nitratee0
- Outcome
- production of anthocyanine0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best nitrogen source for the production of anthocyanin was the combination of 10 mM ammonium and 18.8 mM nitrate as well as 5 mM ammonium and 25 mM nitrate.”
t0“Apple”
The same questions in other papers
magnesium atom · quality: phytochemicals · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- positive
nitrogen · quality: phytochemicals · Malus domestica (apple)Positive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
6 papers more in this group
See all 10 papers →in vitro · quality: phytochemicals · Malus domestica (apple)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.