Abstract
A detailed study was conducted on micropropagation of two elite date palm cvs. Samany and Bertamoda using immature spikelet explants. Initially the callus cultures were established using spikelet explants of immature spathes (avg. 15 cm). Initiation medium containing 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 3.0 mg L-1 2-isopentenyl adenine (2iP) induced significantly highest callus in cv. Samany (95%) followed by cv. Bertamoda (81%). Medium containing micro and macronutrients of MS, vitamins of MS, 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 200 mg L-1 L-Glutamine, 0.05 mg L-1 2,4-D, 3.0 mg L-1 2iP, 3 g L-1 Activated Charcoal (AC) induced white friable embryogenic callus in cv. Samany (84%) and in cv. Bertamoda (69%). Significantly highest multiplication of somatic embryos was obtained in cv. Samany (85%) and in cv. Bertamoda (76%) on the medium containing 0.1 mg L-1 naphthalene acetic acid (NAA), 0.1 mg L-1 Kinetin (Kin) under light. Leaf number, leaf length, root number and root length as significantly highest values were obtained on the medium containing 0.1 mg L-1 BA, 0.1 mg L-1 NAA. Root trimming of primary single root resulted in production of multiple roots in each plantlet. The in vitro hardening supported to select healthy plantlets to be able to grow vigorously in greenhouse. Soil mixture based on peatmoss, river sand, hill sand (4:1:1 v/v) resulted in significantly highest survival (91%) of plantlets in greenhouse during acclimatization. Micropropagated plants (45 cm in height) with 3-4 compound leaves grown in the greenhouse were transferred in the open field for tree and fruit evaluation. Results showed that cvs. Samany and Bertamoda produced true-to-type fruits after three years of plantations in open field. Fruits of cv. Samany were harvested at late khalal stage and utilized to make Chhuhara (boiled/ dried dates) which showed excellent quality. Micropropagation of elite date cultivars is need of the time which will boost varietal structure in the area.
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Claims 12
Claim · model-written summary
The initiation medium significantly induced the highest callus in cv. Samany (95%).
- Taxon
- date palm
Phoenix dactyliferat0 - Material
- cv. Samanye1
- Treatment
- 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 3.0 mg L-1 2-isopentenyl adenine (2iP)e1
- Outcome
- calluse1
- Direction
- significantly higheste1
- Value
- 95%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e1“Initiation medium containing 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 3.0 mg L-1 2-isopentenyl adenine (2iP) induced significantly highest callus in cv. Samany (95%) followed by cv. Bertamoda (81%)”
Claim · model-written summary
Callus in cv. Bertamoda (81%) followed cv. Samany on the initiation medium.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Bertamodae1
- Treatment
- 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 3.0 mg L-1 2-isopentenyl adenine (2iP)e1
- Outcome
- calluse1
- Direction
- followed bye1
- Value
- 81%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e1“Initiation medium containing 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 3.0 mg L-1 2-isopentenyl adenine (2iP) induced significantly highest callus in cv. Samany (95%) followed by cv. Bertamoda (81%)”
Claim · model-written summary
Activated charcoal medium induced white friable embryogenic callus in cv. Samany (84%).
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Samanye2
- Treatment
- 3 g L-1 Activated Charcoal (AC)e2
- Outcome
- white friable embryogenic calluse2
- Direction
- inducede2
- Value
- 84%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e2“Medium containing micro and macronutrients of MS, vitamins of MS, 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 200 mg L-1 L-Glutamine, 0.05 mg L-1 2,4-D, 3.0 mg L-1 2iP, 3 g L-1 Activated Charcoal (AC) induced white friable embryogenic callus in cv. Samany (84%) and in cv. Bertamoda (69%)”
Claim · model-written summary
Activated charcoal medium induced white friable embryogenic callus in cv. Bertamoda (69%).
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Bertamodae2
- Treatment
- 3 g L-1 Activated Charcoal (AC)e2
- Outcome
- white friable embryogenic calluse2
- Direction
- inducede2
- Value
- 69%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e2“Medium containing micro and macronutrients of MS, vitamins of MS, 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 200 mg L-1 L-Glutamine, 0.05 mg L-1 2,4-D, 3.0 mg L-1 2iP, 3 g L-1 Activated Charcoal (AC) induced white friable embryogenic callus in cv. Samany (84%) and in cv. Bertamoda (69%)”
Claim · model-written summary
NAA and kinetin medium gave the highest somatic embryo multiplication in cv. Samany (85%) under light.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Samanye3
- Treatment
- 0.1 mg L-1 naphthalene acetic acid (NAA), 0.1 mg L-1 Kinetin (Kin)e3
- Outcome
- multiplication of somatic embryose3
- Direction
- Significantly higheste3
- Value
- 85%e3
- Setting
- under lighte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e3“Significantly highest multiplication of somatic embryos was obtained in cv. Samany (85%) and in cv. Bertamoda (76%) on the medium containing 0.1 mg L-1 naphthalene acetic acid (NAA), 0.1 mg L-1 Kinetin (Kin) under light”
Claim · model-written summary
NAA and kinetin medium gave the highest somatic embryo multiplication in cv. Bertamoda (76%) under light.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Bertamodae3
- Treatment
- 0.1 mg L-1 naphthalene acetic acid (NAA), 0.1 mg L-1 Kinetin (Kin)e3
- Outcome
- multiplication of somatic embryose3
- Direction
- Significantly higheste3
- Value
- 76%e3
- Setting
- under lighte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e3“Significantly highest multiplication of somatic embryos was obtained in cv. Samany (85%) and in cv. Bertamoda (76%) on the medium containing 0.1 mg L-1 naphthalene acetic acid (NAA), 0.1 mg L-1 Kinetin (Kin) under light”
Claim · model-written summary
BA and NAA medium gave the highest leaf number and leaf length.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- 0.1 mg L-1 BA, 0.1 mg L-1 NAAe4
- Outcome
- Leaf number, leaf lengthe4
- Direction
- significantly higheste4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e4“Leaf number, leaf length, root number and root length as significantly highest values were obtained on the medium containing 0.1 mg L-1 BA, 0.1 mg L-1 NAA”
Claim · model-written summary
BA and NAA medium gave the highest root number and root length.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- 0.1 mg L-1 BA, 0.1 mg L-1 NAAe4
- Outcome
- root number and root lengthe4
- Direction
- significantly higheste4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e4“Leaf number, leaf length, root number and root length as significantly highest values were obtained on the medium containing 0.1 mg L-1 BA, 0.1 mg L-1 NAA”
Claim · model-written summary
Root trimming of the primary single root resulted in multiple roots per plantlet.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- Root trimming of primary single roote5
- Outcome
- production of multiple rootse5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e5“Root trimming of primary single root resulted in production of multiple roots in each plantlet”
Claim · model-written summary
The peatmoss, river sand and hill sand mixture gave the highest survival (91%) of plantlets in the greenhouse.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- Soil mixture based on peatmoss, river sand, hill sand (4:1:1 v/v)e6
- Outcome
- survivale6
- Direction
- significantly higheste6
- Value
- 91%e6
- Setting
- greenhousee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e6“Soil mixture based on peatmoss, river sand, hill sand (4:1:1 v/v) resulted in significantly highest survival (91%) of plantlets in greenhouse during acclimatization”
Claim · model-written summary
cvs. Samany and Bertamoda produced true-to-type fruits after three years in the open field.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cvs. Samany and Bertamodae7
- Outcome
- true-to-type fruitse7
- Setting
- open fielde7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e7“Results showed that cvs. Samany and Bertamoda produced true-to-type fruits after three years of plantations in open field. Fruits of cv. Samany were harvested at late khalal stage and utilized to make Chhuhara (boiled/ dried dates) which showed excellent quality”
Claim · model-written summary
Fruits of cv. Samany made into Chhuhara showed excellent quality.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Samanye7
- Outcome
- excellent qualitye7
- Qualifier
- Chhuhara (boiled/ dried dates)e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of elite date palm (Phoenix dactylifera L.) cultivars Samany and Bertamoda through immature inflorescence explants”
e7“Results showed that cvs. Samany and Bertamoda produced true-to-type fruits after three years of plantations in open field. Fruits of cv. Samany were harvested at late khalal stage and utilized to make Chhuhara (boiled/ dried dates) which showed excellent quality”
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