Abstract
Studies were conducted to test the effect of different growth regulators, sucrose and nitrogen on Phoenix dactylifera L. explants cultured on Eeuwen’s basal medium. Naphthalene acetic acid (NAA) was very effective for callus induction. Addition of cytokinins (BAP and Kinetin) to NAA containing media did not enhance actual callus growth. Sucrose influenced callus production. Depending on the auxin concentration of media, callus production could be supported by sucrose within the range 15 - 105 g/l but the optimum sucrose concentration in the medium in all cases, as determined by size of callus was 30 g/l. NAA and sucrose tended to interact at relatively high levels of sucrose (45 – 90 g/l) to produce roots in culture. KNO3 was essential as a source of nitrogen for callogenesis and optimum callus formation was observed at 50 mM (combined nitrogen). Key words: Phoenix dactylifera, in vitro culture, phytohormone, sucrose.
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Claims 8
Claim · model-written summary
NAA effectively induced callus.
- Taxon
- Phoenix dactylifera L.
Phoenix dactyliferat0 - Treatment
- Naphthalene acetic acid (NAA)e0
- Outcome
- callus inductione0
- Direction
- very effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Naphthalene acetic acid (NAA) was very effective for callus induction”
t0“Phoenix dactylifera L.”
Claim · model-written summary
BAP addition to NAA media did not enhance callus growth.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- BAPe0
- Outcome
- actual callus growthe0
- Direction
- did not enhancee0
- Comparator
- NAA containing mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of cytokinins (BAP and Kinetin) to NAA containing media did not enhance actual callus growth”
t0“Phoenix dactylifera L.”
Claim · model-written summary
Kinetin addition to NAA media did not enhance callus growth.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- Kinetine0
- Outcome
- actual callus growthe0
- Direction
- did not enhancee0
- Comparator
- NAA containing mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of cytokinins (BAP and Kinetin) to NAA containing media did not enhance actual callus growth”
t0“Phoenix dactylifera L.”
Claim · model-written summary
Sucrose supported callus production at 15 - 105 g/l depending on auxin.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- sucrose within the range 15 - 105 g/le0
- Outcome
- callus productione0
- Direction
- supportede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus production could be supported by sucrose within the range 15 - 105 g/l”
t0“Phoenix dactylifera L.”
Claim · model-written summary
Optimal sucrose concentration for callus size was 30 g/l.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- sucrose concentratione0
- Outcome
- size of calluse0
- Direction
- optimume0
- Value
- 30 g/le0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimum sucrose concentration in the medium in all cases, as determined by size of callus was 30 g/l”
t0“Phoenix dactylifera L.”
Claim · model-written summary
NAA with 45 – 90 g/l sucrose tended to produce roots in culture.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- NAA and sucrosee0
- Outcome
- produce roots in culturee0
- Value
- 45 – 90 g/le0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAA and sucrose tended to interact at relatively high levels of sucrose (45 – 90 g/l) to produce roots in culture”
t0“Phoenix dactylifera L.”
Claim · model-written summary
KNO3 was essential for callogenesis.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- KNO3e0
- Outcome
- callogenesise0
- Direction
- essentiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KNO3 was essential as a source of nitrogen for callogenesis”
t0“Phoenix dactylifera L.”
Claim · model-written summary
Optimal callus formation occurred at 50 mM combined nitrogen.
- Taxon
- Phoenix dactylifera L.Phoenix dactyliferat0
- Treatment
- 50 mM (combined nitrogen)e0
- Outcome
- callus formatione0
- Direction
- optimume0
- Value
- 50 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“optimum callus formation was observed at 50 mM (combined nitrogen)”
t0“Phoenix dactylifera L.”
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