Abstract
Micropropagation of date palm (Phoenix dactylifera L. cv. Samany) through somatic embryogenesis and organogenesis methods have been studied in this investigation. Aged tree and juvenile offshoot of date palm were used as source of explants. Shoot tips and leaf primordia were isolated from those two sources to be used as two types of explants. The response of the two types of explants was examined when they cultured on two different nutrient media (M1 and M2), where M1 medium was modified MS contained NOA (5mg/l), NAA (5mg/l), Kin (3mg/l) and 2iP (3mg/l) and M2 medium was MS contained 2,4-D (100 mg/l) and 2IP (3 mg/l). Results indicated that there was no significant difference between shoot tips and leaf primordia derived from either aged tree or juvenile offshoot for their effect on survival percentage, while, the degree of oxidative browning on M1 medium was significantly lower than M2 medium. After 8 weeks or even 4 months, all examined explants formed callus on M2 medium as compared to M1 medium, which showed no callus formation. Interestingly, results proved that leaf primordia of both sources cultured on M1 medium stimulated direct organogenesis and somatic embryogenesis after 4 months of incubation. In that concern, shoot tips of both sources did not observe any direct morphogenetic responses. Growth and development of the previously obtained adventitious buds as well as repetitive somatic embryos have been optimized on hormone-free M1 medium. Roots were induced in MS basal medium contained NAA (0.2 m /L), and mostly, the addition of charcoal to the rooting medium had no visible effect on root formation. At acclimatization stage, it was found that, using of induced the maximum survival rate and growth vigor for plantlets after 3 months under greenhouse conditions compared to those produced under the other tested soil mixtures. Finally, results strongly reflected that, leaf primordia of aged tree or offshoots are a promising explants on M1 medium for the mass production of true-to- type plants since the callus stage is avoided.
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Claims 9
Claim · model-written summary
Explant type and source did not significantly differ in survival.
- Taxon
- date palm
Phoenix dactyliferae1 - Organ / stage
- shoot tips and leaf primordiae0
- Outcome
- survival percentagee0
- Direction
- no significant differencee0
- Comparator
- aged tree or juvenile offshoote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no significant difference between shoot tips and leaf primordia derived from either aged tree or juvenile offshoot for their effect on survival percentage”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
M1 medium caused less oxidative browning than M2.
- Taxon
- date palmPhoenix dactyliferae1
- Treatment
- M1 mediume0
- Outcome
- degree of oxidative browninge0
- Direction
- lowere0
- Comparator
- M2 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the degree of oxidative browning on M1 medium was significantly lower than M2 medium”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
All explants formed callus on M2, while none did on M1.
- Taxon
- date palmPhoenix dactyliferae1
- Treatment
- M2 mediume0
- Outcome
- formed calluse0
- Comparator
- M1 mediume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all examined explants formed callus on M2 medium as compared to M1 medium, which showed no callus formation”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
Leaf primordia from both sources on M1 underwent direct organogenesis and somatic embryogenesis after four months.
- Taxon
- date palmPhoenix dactyliferae1
- Organ / stage
- leaf primordiae0
- Treatment
- M1 mediume0
- Outcome
- direct organogenesis and somatic embryogenesise0
- Qualifier
- after 4 months of incubatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leaf primordia of both sources cultured on M1 medium stimulated direct organogenesis and somatic embryogenesis after 4 months of incubation”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
Shoot tips from both sources showed no direct morphogenetic response.
- Taxon
- date palmPhoenix dactyliferae1
- Organ / stage
- shoot tipse0
- Outcome
- did not observe any direct morphogenetic responsese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot tips of both sources did not observe any direct morphogenetic responses”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
Hormone-free M1 optimized development of adventitious buds and repetitive somatic embryos.
- Taxon
- date palmPhoenix dactyliferae1
- Treatment
- hormone-free M1 mediume0
- Outcome
- Growth and development of the previously obtained adventitious buds as well as repetitive somatic embryose0
- Direction
- optimizede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth and development of the previously obtained adventitious buds as well as repetitive somatic embryos have been optimized on hormone-free M1 medium”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
Roots were induced on MS basal medium with NAA.
- Taxon
- date palmPhoenix dactyliferae1
- Treatment
- MS basal medium contained NAA (0.2 m /L)e0
- Outcome
- Roots were inducede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Roots were induced in MS basal medium contained NAA (0.2 m /L)”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
Charcoal addition had no visible effect on root formation.
- Taxon
- date palmPhoenix dactyliferae1
- Treatment
- addition of charcoal to the rooting mediume0
- Outcome
- root formatione0
- Direction
- no visible effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of charcoal to the rooting medium had no visible effect on root formation”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
Claim · model-written summary
Leaf primordia from aged trees or offshoots on M1 were recommended for true-to-type mass production.
- Taxon
- date palmPhoenix dactyliferae1
- Organ / stage
- leaf primordiae0
- Treatment
- M1 mediume0
- Outcome
- mass production of true-to- type plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leaf primordia of aged tree or offshoots are a promising explants on M1 medium for the mass production of true-to- type plants since the callus stage is avoided”
e1“date palm (Phoenix dactylifera L. cv. Samany)”
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See all 51 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.