Paper · 2025 · Kashmir Journal of Science

Somatic embryogenesis regulating shoot regeneration efficiency of Date palm (Phoenix dactylifera L.)

Allah Jurio Khaskheli; Shahla Baloch

8
claims
18
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-nc

Abstract

The present study was conducted to estimate the specific potential of regenerated date palm by somatic embryogenesis of tissue culture technique. Collectively total of thirty rooted offshoots were cultured on basal medium complemented with composition of 0.0, 0.5, 1.0, 2.0, 3.0 and 4.0 mg / L of BA and Kinetin. The interpretations on the existence time interval, days reserved to start shoot initiation, total shoots number and shoots length were noted. Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) exhibited a significantly lower survival rate (72.50% P < 0.05) and no shoot formation was noticed throughout the experimental duration. The highest concentration (4.0 mg/L) resulted in the shortest time for shoot initiation (17.50 days), whereas lower concentrations (0.5 and 1.0 mg/L) exhibited extended durations (23.50 and 24.20 days, respectively). The maximum number of shoots per explant (5.36) was achieved with 4.0 mg/L BA + Kin, while the concentration of 2.0 mg/L produced an optimal number of shoots (3.30). This study highlights the significance of optimizing BA and Kin concentrations to enhance somatic embryogenesis shoot regeneration efficiency in date palm through tissue culture techniques.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

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Claims 8

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Date palm cultured without BAP and kinetin had a significantly lower survival rate, 72.50%.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
without the addition of BAP (6-benzylaminopurine) and kinetin (Kin)e1
Outcome
survival ratee1
Direction
significantly lowere1
Value
72.50%e1
Qualifier
P < 0.05e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e1

“Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) exhibited a significantly lower survival rate (72.50% P < 0.05) and no shoot formation was noticed throughout the experimental duration”

✓ located verbatim in the source · abstract, characters 456–733 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Date palm cultured without BAP and kinetin formed no shoots.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
without the addition of BAP (6-benzylaminopurine) and kinetin (Kin)e1
Outcome
no shoot formatione1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e1

“Date palm (Phoenix dactylifera L.) culture redeveloped on MS basal medium without the addition of BAP (6-benzylaminopurine) and kinetin (Kin) exhibited a significantly lower survival rate (72.50% P < 0.05) and no shoot formation was noticed throughout the experimental duration”

✓ located verbatim in the source · abstract, characters 456–733 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest concentration, 4.0 mg/L, gave the shortest time for shoot initiation, 17.50 days.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
The highest concentration (4.0 mg/L)e2
Outcome
time for shoot initiatione2
Direction
shorteste2
Value
17.50 dayse2
Comparator
lower concentrations (0.5 and 1.0 mg/L)e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e2

“The highest concentration (4.0 mg/L) resulted in the shortest time for shoot initiation (17.50 days)”

✓ located verbatim in the source · abstract, characters 735–835 · show in text ↑
e3

“whereas lower concentrations (0.5 and 1.0 mg/L) exhibited extended durations (23.50 and 24.20 days, respectively)”

✓ located verbatim in the source · abstract, characters 837–950 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Lower concentrations of 0.5 and 1.0 mg/L extended shoot initiation duration, to 23.50 days at 0.5 mg/L.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
lower concentrations (0.5 and 1.0 mg/L)e3
Outcome
durationse3
Direction
extendede3
Value
23.50e3
Comparator
The highest concentration (4.0 mg/L)e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e2

“The highest concentration (4.0 mg/L) resulted in the shortest time for shoot initiation (17.50 days)”

✓ located verbatim in the source · abstract, characters 735–835 · show in text ↑
e3

“whereas lower concentrations (0.5 and 1.0 mg/L) exhibited extended durations (23.50 and 24.20 days, respectively)”

✓ located verbatim in the source · abstract, characters 837–950 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Lower concentrations of 0.5 and 1.0 mg/L extended shoot initiation duration, to 24.20 days at 1.0 mg/L.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
lower concentrations (0.5 and 1.0 mg/L)e3
Outcome
durationse3
Direction
extendede3
Value
24.20 dayse3
Comparator
The highest concentration (4.0 mg/L)e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e2

“The highest concentration (4.0 mg/L) resulted in the shortest time for shoot initiation (17.50 days)”

✓ located verbatim in the source · abstract, characters 735–835 · show in text ↑
e3

“whereas lower concentrations (0.5 and 1.0 mg/L) exhibited extended durations (23.50 and 24.20 days, respectively)”

✓ located verbatim in the source · abstract, characters 837–950 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The maximum number of shoots per explant, 5.36, was achieved with 4.0 mg/L BA plus kinetin.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
4.0 mg/L BA + Kine4
Outcome
number of shoots per explante4
Direction
maximume4
Value
5.36e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e4

“The maximum number of shoots per explant (5.36) was achieved with 4.0 mg/L BA + Kin”

✓ located verbatim in the source · abstract, characters 952–1035 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The concentration of 2.0 mg/L produced an optimal number of shoots, 3.30.

Taxon
Date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
Treatment
the concentration of 2.0 mg/Le5
Outcome
number of shootse5
Direction
optimale5
Value
3.30e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 66–100 · show in text ↑
e5

“the concentration of 2.0 mg/L produced an optimal number of shoots (3.30)”

✓ located verbatim in the source · abstract, characters 1043–1116 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Optimizing BA and kinetin concentrations is recommended to enhance shoot regeneration efficiency in date palm.

Taxon
date palmPhoenix dactyliferae6
Outcome
optimizing BA and Kin concentrationse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e6

“This study highlights the significance of optimizing BA and Kin concentrations to enhance somatic embryogenesis shoot regeneration efficiency in date palm”

✓ located verbatim in the source · abstract, characters 1118–1272 · show in text ↑

N-benzyladenine · survival · Phoenix dactylifera (date palm)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

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kinetin · survival · Phoenix dactylifera (date palm)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

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kinetin · growth · Phoenix dactylifera (date palm)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

1 paper in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
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N-benzyladenine · growth · Phoenix dactylifera (date palm)Positive and negative results

23 papers: 21 positive, 2 negative or no effect

1 paper in the same direction as this paper · 21 in the opposite direction

  1. positive
  2. positive
  3. positive

19 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.