Abstract
Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long tender leaf base explants derived from the meristamatic region of 2-3 year old offshoots of date palm cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 and 150 mg l -1 2,4-dichlorophenoxy acetic acid (2,4-D) and incubated in dark for 6 weeks to initiate callus. Callogenesis was obtained in all 2,4-D concentrations tested; however, callus growth was most significant in media supplemented with 100 mg l -1 2,4-D. The leaf explants with callus were transferred to hormone-free MS medium for 4 weeks and then further sub-cultured to a medium supplemented with 0.5 mg l -1 -naphthalene acetic acid (NAA) and 0.25 mg l -1 6-benzyl amino purine (BAP) which was effective in inducing shoot and root primordia within 10 weeks. In another 12 weeks, two more sub-culturing of shoot clumps in the same medium resulted in the development of shoot with roots and gave whole plants by 8 weeks. The plantlets were hardened and acclimatized to the ambient conditions and planted in pots, containing 1:1:1 peat, sand and dehydrated cow manure, which resulted in over 60% ex vitro plant survival. Early plant regeneration was achieved by this technique.
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Claims 7
Claim · model-written summary
Tender leaf base explants underwent somatic embryogenesis.
- Taxon
- date palm (Phoenix dactylifera L.)
Phoenix dactyliferae0 - Organ / stage
- tender leaf base explante0
- Outcome
- Somatic embryogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.).”
Claim · model-written summary
Callus formed at every tested 2,4-D concentration.
- Taxon
- date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
- Treatment
- 2,4-D concentrationse0
- Outcome
- Callogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callogenesis was obtained in all 2,4-D concentrations tested”
t0“date palm (Phoenix dactylifera L.)”
Claim · model-written summary
Callus growth was greatest with 100 mg l -1 2,4-D.
- Taxon
- date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
- Treatment
- 100 mg l -1 2,4-De0
- Outcome
- callus growthe0
- Direction
- most significante0
- Comparator
- all 2,4-D concentrations testedt1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus growth was most significant in media supplemented with 100 mg l -1 2,4-D.”
t0“date palm (Phoenix dactylifera L.)”
t1“all 2,4-D concentrations tested”
Claim · model-written summary
NAA and BAP medium induced shoot and root primordia within 10 weeks.
- Taxon
- date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
- Treatment
- 0.5 mg l -1 -naphthalene acetic acid (NAA) and 0.25 mg l -1 6-benzyl amino purine (BAP)e0
- Outcome
- shoot and root primordiae0
- Value
- within 10 weekse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a medium supplemented with 0.5 mg l -1 -naphthalene acetic acid (NAA) and 0.25 mg l -1 6-benzyl amino purine (BAP) which was effective in inducing shoot and root primordia within 10 weeks.”
t0“date palm (Phoenix dactylifera L.)”
Claim · model-written summary
Two further subcultures produced rooted shoots and whole plants.
- Taxon
- date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
- Treatment
- two more sub-culturing of shoot clumpse0
- Outcome
- development of shoot with roots and gave whole plantse0
- Value
- by 8 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two more sub-culturing of shoot clumps in the same medium resulted in the development of shoot with roots and gave whole plants by 8 weeks.”
t0“date palm (Phoenix dactylifera L.)”
Claim · model-written summary
Potting acclimatized plantlets in peat, sand and cow manure yielded over 60% ex vitro survival.
- Taxon
- date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
- Treatment
- 1:1:1 peat, sand and dehydrated cow manuree0
- Outcome
- ex vitro plant survivale0
- Value
- over 60%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“planted in pots, containing 1:1:1 peat, sand and dehydrated cow manure, which resulted in over 60% ex vitro plant survival.”
t0“date palm (Phoenix dactylifera L.)”
Claim · model-written summary
The technique achieved early plant regeneration.
- Taxon
- date palm (Phoenix dactylifera L.)Phoenix dactyliferat0
- Outcome
- Early plant regeneratione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Early plant regeneration was achieved by this technique.”
t0“date palm (Phoenix dactylifera L.)”
The same questions in other papers
2,4-D · growth · Phoenix dactylifera (date palm)Positive and negative results
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- positive
- positive
- negative
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See all 25 papers →fertilization · survival · Phoenix dactylifera (date palm)Results agree
2 papers in the same direction as this paper
- positive
- positive
growing medium · survival · Phoenix dactylifera (date palm)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.