Abstract
In sorghum [Sorghum bicolor (L.) Moench], developed protocols for plant regeneration via somatic embryogenesis do not include maturation stage. The present work was carried out with the aim of achieving the maturation and germination of sorghum somatic embryos in cultivar 'CIAP 132R-05'. It were studied four concentrations of sucrose (30, 50, 70 and 90 g l-1), two of abscisic acid (0.25 and 0.5 μM) and a control without this growth regulator. Germination initiation (days) and number of somatic embryos with complete germination were evaluated in three periods (1 - 7, 8 - 14 and 15 - 21 days of culture). In addition, the effect of 6-BAP (8.9, 17.8 and 26.6 μM) on somatic embryo germination was determined. The germination start time (days) and after 21 days the number of somatic embryos with complete germination and plants with malformations were determined. The addition of 70 g l-1 sucrose in the culture medium without abscisic acid increased the germination of the somatic embryos to 37.2 plants per embryo group (0.5 g of fresh mass). The highest number of somatic embryos germinated was obtained with 17.78 μM 6-BAP in the germination culture medium. It was demonstrated the need of a maturation stage in the sorghum somatic embryogenesis to increase the germination percentage. Keywords: somatic embryogenesis, sorghum, sucrose, 6-BAP
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Claims 3
Claim · model-written summary
Medium with 70 g l-1 sucrose and no abscisic acid raised somatic embryo germination to 37.2 plants per group.
- Taxon
- Sorghum bicolor (L.) Moench
Sorghum bicolore1 - Treatment
- 70 g l-1 sucrose in the culture medium without abscisic acide0
- Outcome
- germination of the somatic embryose0
- Direction
- increasede0
- Value
- 37.2 plants per embryo groupe0
- Qualifier
- 0.5 g of fresh masse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of 70 g l-1 sucrose in the culture medium without abscisic acid increased the germination of the somatic embryos to 37.2 plants per embryo group (0.5 g of fresh mass)”
e1“In sorghum [Sorghum bicolor (L.) Moench]”
Claim · model-written summary
The highest number of germinated somatic embryos occurred with 17.78 μM 6-BAP.
- Taxon
- Sorghum bicolor (L.) MoenchSorghum bicolore1
- Treatment
- 17.78 μM 6-BAPe0
- Outcome
- number of somatic embryos germinatede0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of somatic embryos germinated was obtained with 17.78 μM 6-BAP in the germination culture medium”
e1“In sorghum [Sorghum bicolor (L.) Moench]”
Claim · model-written summary
The authors concluded that a maturation stage is needed to increase sorghum somatic embryo germination percentage.
- Outcome
- maturation stage in the sorghum somatic embryogenesis to increase the germination percentagee0
SorghumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was demonstrated the need of a maturation stage in the sorghum somatic embryogenesis to increase the germination percentage”
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