Abstract
Novel protocols for production of multiple shoot-tip clumps and somatic embryos of Sorghum bicolor (L.) Moench were developed with long-term goal of crop improvement through genetic transformation. Multiple shoot-tip clumps were developed in vitro from shoot-tip explant of one-week old seedling, cultured on MS medium containing only BA (0.5, 1 or 2 mg/l) or both BA (1 or 2 mg/l) and 2,4-D (0.5 mg/l) with bi-weekly subculture. Somatic embryos were directly produced on the enlarged dome shaped growing structures that developed from the shoot-tips of one-week old seedling explants (without any callus formation) when cultured on MS medium supplemented with both 2,4-D (0.5 mg/l) and BA (0.5 mg/l). However, the supplementation of MS medium with only 2,4-D (0.5 mg/l) induced compact callus without any plantlet regeneration. Each multiple shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing indole-3-butyric acid (IBA 1 mg/l). The plants were successfully transplanted to glasshouse and grown to maturity with a survival rate of 98%. Morphogenetic response of the explants was found to be genotypically independent.
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Claims 8
Claim · model-written summary
BA alone or with 2,4-D produced multiple shoot-tip clumps from sorghum explants.
- Taxon
- Sorghum bicolor
Sorghum bicolort0 - Organ / stage
- shoot-tip explante0
- Treatment
- MS medium containing only BA (0.5, 1 or 2 mg/l) or both BA (1 or 2 mg/l) and 2,4-D (0.5 mg/l)e0
- Outcome
- Multiple shoot-tip clumpse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Multiple shoot-tip clumps were developed in vitro from shoot-tip explant of one-week old seedling, cultured on MS medium containing only BA (0.5, 1 or 2 mg/l) or both BA (1 or 2 mg/l) and 2,4-D (0.5 mg/l) with bi-weekly subculture.”
t0“Sorghum bicolor”
Claim · model-written summary
Combined 2,4-D and BA directly produced somatic embryos without callus.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Organ / stage
- shoot-tips of one-week old seedling explantse0
- Treatment
- MS medium supplemented with both 2,4-D (0.5 mg/l) and BA (0.5 mg/l)e0
- Outcome
- Somatic embryose0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos were directly produced on the enlarged dome shaped growing structures that developed from the shoot-tips of one-week old seedling explants (without any callus formation) when cultured on MS medium supplemented with both 2,4-D (0.5 mg/l) and BA (0.5 mg/l).”
t0“Sorghum bicolor”
Claim · model-written summary
2,4-D alone induced compact callus without plantlet regeneration.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Treatment
- MS medium with only 2,4-D (0.5 mg/l)e0
- Outcome
- compact callus without any plantlet regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the supplementation of MS medium with only 2,4-D (0.5 mg/l) induced compact callus without any plantlet regeneration”
t0“Sorghum bicolor”
Claim · model-written summary
Each multiple shoot-clump regenerated more than 80 shoots.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Outcome
- shootse0
- Direction
- regeneratinge0
- Value
- more than 80 shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Each multiple shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds”
t0“Sorghum bicolor”
Claim · model-written summary
Somatic embryos achieved 90% germination, plant conversion and regeneration.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Outcome
- germination, plant conversion and regeneratione0
- Value
- 90%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the somatic embryos were capable of 90% germination, plant conversion and regeneration”
t0“Sorghum bicolor”
Claim · model-written summary
IBA medium efficiently rooted regenerated shoots.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Treatment
- MS medium containing indole-3-butyric acid (IBA 1 mg/l)e0
- Outcome
- regenerated shoots could be efficiently rootede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated shoots could be efficiently rooted on MS medium containing indole-3-butyric acid (IBA 1 mg/l).”
t0“Sorghum bicolor”
Claim · model-written summary
Transplanted plants reached maturity with 98% survival.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Outcome
- survival ratee0
- Value
- 98%e0
- Setting
- glasshousee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plants were successfully transplanted to glasshouse and grown to maturity with a survival rate of 98%.”
t0“Sorghum bicolor”
Claim · model-written summary
Morphogenetic response was genotypically independent.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Outcome
- Morphogenetic response of the explants was found to be genotypically independente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Morphogenetic response of the explants was found to be genotypically independent.”
t0“Sorghum bicolor”
The same questions in other papers
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- positive
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N-benzyladenine · growth · Sorghum bicolor (sorghum)Results agree
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9 papers in the same direction as this paper · 1 in the opposite direction
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.