Abstract
In this study the effect of different colchicine concentrations (0.025, 0.05, 0.1 and 0.2%) and treatment time (8, 24 and 48 h) on in vitro polyploidy induction from shoot tips was investigated as a factorial experiment based on completely randomized design with three replications. Polyploidy induction was confirmed by chromosome counts, size and number of stomata and other morphological characters. By assessment of different media, it was revealed that the suitable medium for regeneration of shoot tips is MS medium supplemented with 0.1 mg L−1 IBA (indole-3-butyric acid) and 1 or 2 mg L−1 BAP (6-benzylaminopurine) or 2 mg L−1 TDZ (thidiazuron). In addition, MS medium supplemented with 1 mg L−1 NAA (1-naphthaleneacetic acid) and 0.5 or 1 mg L−1 IBA led to the maximum rooting of plantlets. With increasing colchicine concentration and its treatment duration, explants survival and their rooting considerably decreased. The results revealed that 0.1% concentration of colchicine with 48 h of treatment established the maximum amount of the in vitro induced tetraploid plantlets. The derived tetraploid plantlets had bigger stomata with lower density. Chlorophyll content in tetraploid plantlets was significantly higher than diploids, and they also showed an elevated level of antioxidant enzymes, protein and soluble carbohydrate contents as compared with diploids.
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Claims 11
Claim · model-written summary
MS with IBA and BAP or TDZ was suitable for shoot tip regeneration.
- Taxon
- Sorghum bicolor L.
Sorghum bicolort0 - Treatment
- MS medium supplemented with 0.1 mg L−1 IBA (indole-3-butyric acid) and 1 or 2 mg L−1 BAP (6-benzylaminopurine) or 2 mg L−1 TDZ (thidiazuron)e0
- Outcome
- regeneration of shoot tipse0
- Direction
- suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suitable medium for regeneration of shoot tips is MS medium supplemented with 0.1 mg L−1 IBA (indole-3-butyric acid) and 1 or 2 mg L−1 BAP (6-benzylaminopurine) or 2 mg L−1 TDZ (thidiazuron)”
t0“Sorghum bicolor L.”
Claim · model-written summary
MS with NAA and IBA gave maximum plantlet rooting.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Treatment
- MS medium supplemented with 1 mg L−1 NAA (1-naphthaleneacetic acid) and 0.5 or 1 mg L−1 IBAe0
- Outcome
- rooting of plantletse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1 mg L−1 NAA (1-naphthaleneacetic acid) and 0.5 or 1 mg L−1 IBA led to the maximum rooting of plantlets”
t0“Sorghum bicolor L.”
Claim · model-written summary
Increasing colchicine concentration reduced explant survival.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Treatment
- colchicine concentratione0
- Outcome
- explants survivale0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With increasing colchicine concentration and its treatment duration, explants survival and their rooting considerably decreased”
t0“Sorghum bicolor L.”
Claim · model-written summary
Longer colchicine treatment reduced explant rooting.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Treatment
- treatment duratione0
- Outcome
- rootinge0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With increasing colchicine concentration and its treatment duration, explants survival and their rooting considerably decreased”
t0“Sorghum bicolor L.”
Claim · model-written summary
0.1% colchicine for 48 hours maximized induced tetraploid plantlets.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Treatment
- 0.1% concentration of colchicine with 48 h of treatmente0
- Outcome
- in vitro induced tetraploid plantletse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.1% concentration of colchicine with 48 h of treatment established the maximum amount of the in vitro induced tetraploid plantlets”
t0“Sorghum bicolor L.”
Claim · model-written summary
Tetraploid plantlets had larger stomata than diploids.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Material
- tetraploid plantletse0
- Outcome
- stomatae0
- Direction
- biggere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The derived tetraploid plantlets had bigger stomata with lower density”
t0“Sorghum bicolor L.”
Claim · model-written summary
Tetraploid plantlets had lower stomatal density than diploids.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Material
- tetraploid plantletse0
- Outcome
- densitye0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The derived tetraploid plantlets had bigger stomata with lower density”
t0“Sorghum bicolor L.”
Claim · model-written summary
Tetraploid plantlets had significantly higher chlorophyll than diploids.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Material
- tetraploid plantletse0
- Outcome
- Chlorophyll contente0
- Direction
- highere0
- Comparator
- diploidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chlorophyll content in tetraploid plantlets was significantly higher than diploids”
t0“Sorghum bicolor L.”
Claim · model-written summary
Tetraploid plantlets had higher antioxidant enzymes than diploids.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Material
- tetraploid plantletse1
- Outcome
- antioxidant enzymese0
- Direction
- elevatede0
- Comparator
- diploidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated level of antioxidant enzymes, protein and soluble carbohydrate contents as compared with diploids”
t0“Sorghum bicolor L.”
e1“The derived tetraploid plantlets had bigger stomata”
Claim · model-written summary
Tetraploid plantlets had higher protein content than diploids.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Outcome
- proteine0
- Direction
- elevatede0
- Comparator
- diploidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated level of antioxidant enzymes, protein and soluble carbohydrate contents as compared with diploids”
t0“Sorghum bicolor L.”
Claim · model-written summary
Tetraploid plantlets had higher soluble carbohydrate content than diploids.
- Taxon
- Sorghum bicolor L.Sorghum bicolort0
- Outcome
- soluble carbohydrate contentse0
- Direction
- elevatede0
- Comparator
- diploidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated level of antioxidant enzymes, protein and soluble carbohydrate contents as compared with diploids”
t0“Sorghum bicolor L.”
The same questions in other papers
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9 papers in the same direction as this paper · 1 in the opposite direction
- no effect
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.