Abstract
Sorghum bicolor is a recalcitrant species for tissue culture regeneration and genetic transformation. Browning of explants is one of the factors limiting organ and tissue cultures. To overcome this, callus tissue was initiated from the shoot tips of in vitro germinating seeds (S. bicolor cv. Róna 1), and then cultured on modified MS media (Murashige and Skoog in Physiol Plant 15:473–497, 1962). In the first experiment, we tested callus induction on several media supplemented with casein hydrolysate, polyvinylpyrrolidone, honey, and sucrose. The best callus induction was recorded for the medium with honey and sucrose (80.0%) and for control medium (79.8%). Shoot regeneration was tested on the MS medium with 6-benzylaminopurine (BAP) supplemented with honey and sucrose at a 1:1 ratio (by weight) or with sucrose only. The highest percentage of calluses regenerating shoots was noted for those induced on the medium with sucrose and honey—approx. four times higher when compared to the control. Rooted plantlets were acclimatized with a 92% survival rate. In the second experiment, we analyzed culture responses to various ways of honey application to the induction media: honey (autoclaved or filtered) in presence or absence of sucrose. Supplementation of the medium with fructose, glucose, and maltose at a proportion typical for honey was also investigated. The explant and callus survival rates were similar to those of the honey–sucrose combination in the first experiment. Only presence of both sucrose and honey in the induction medium improved the total regeneration rate to 37.9% over the control (18.8%). Sucrose and honey appear to act synergistically for shoot regeneration in callus cultures of sorghum.
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Claims 6
Claim · model-written summary
Honey and sucrose medium produced 80.0% callus induction.
- Taxon
- Sorghum bicolor
Sorghum bicolort0 - Treatment
- medium with honey and sucrosee0
- Outcome
- callus inductione0
- Direction
- beste0
- Value
- 80.0%e0
- Qualifier
- 79.8%e0
- Comparator
- control mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best callus induction was recorded for the medium with honey and sucrose (80.0%) and for control medium (79.8%).”
t0“Sorghum bicolor”
Claim · model-written summary
Honey and sucrose induction medium yielded about four times more shoot-regenerating calluses than control.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Treatment
- medium with sucrose and honeye0
- Outcome
- percentage of calluses regenerating shootse0
- Direction
- highere0
- Value
- approx. four timese0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of calluses regenerating shoots was noted for those induced on the medium with sucrose and honey—approx. four times higher when compared to the control.”
t0“Sorghum bicolor”
Claim · model-written summary
Acclimatized rooted plantlets survived at 92%.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Organ / stage
- Rooted plantletse0
- Outcome
- survival ratee0
- Value
- 92%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted plantlets were acclimatized with a 92% survival rate.”
t0“Sorghum bicolor”
Claim · model-written summary
Explant and callus survival rates under second-experiment treatments resembled the honey-sucrose combination.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Treatment
- honey (autoclaved or filtered) in presence or absence of sucrosee1
- Outcome
- explant and callus survival ratese0
- Direction
- similare0
- Comparator
- honey–sucrose combinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The explant and callus survival rates were similar to those of the honey–sucrose combination in the first experiment.”
e1“honey (autoclaved or filtered) in presence or absence of sucrose.”
t0“Sorghum bicolor”
Claim · model-written summary
Only combined sucrose and honey improved total regeneration to 37.9% versus 18.8% control.
- Taxon
- Sorghum bicolorSorghum bicolort0
- Treatment
- presence of both sucrose and honey in the induction mediume0
- Outcome
- total regeneration ratee0
- Direction
- improvede0
- Value
- 37.9%e0
- Qualifier
- 18.8%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only presence of both sucrose and honey in the induction medium improved the total regeneration rate to 37.9% over the control (18.8%).”
t0“Sorghum bicolor”
Claim · model-written summary
The authors infer synergistic action of sucrose and honey on shoot regeneration.
SorghumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose and honey appear to act synergistically for shoot regeneration in callus cultures of sorghum.”
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sucrose · growth · Sorghum bicolor (sorghum)Results agree
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- no effect
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.