Abstract
We find out the antimicrobial potential of partially purified 2,4-diacetylphloroglucinol (DAPG) against Ralstonia solanacearum and fungal plant pathogens isolated from tomato rhizobacterium Pseudomonas fluorescens VSMKU3054. The present study is mainly focused on the control of wilt disease of tomato by our isolate VSMKU3054 and DAPG. The cell free culture filtrate of P. fluorescens VSMKU3054 was significantly arrested the growth of R. solanacearum and fungal pathogens such as Rhizoctonia solani, Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporum compared to control. The existence of DAPG from the crude metabolites of P. fluorescens VSMKU3054 was confirmed on TLC with Rf value 0.34, which is coincide with that of authentic phloroglucinol. The partially purified DAPG exhibited much higher activity against R. solanacearum at 30 µg/ml than the fungal plant pathogens compared to control. The antimicrobial partially purified compound was identified as DAPG by UV, FT-IR and GC–MS analysis. The percentage of live cells of R. solanacearum when supplemented with DAPG at 30 µg/ml, significantly controlled the living nature of R. solanacearum up to 68% compared to tetracycline and universal control observed under high content screening analysis. The selected isolate P. fluorescens VSMKU3054 and DAPG significantly controlled wilt disease of tomato up to 59.5% and 42.12% on 3rd and 7th days compared to positive and negative control by detached leaf assay. Further, in silico analysis revealed that high interaction of DAPG encoding protease with lectin which is associated with R. solanacearum. Based on our findings, we confirmed that P. fluorescens VSMKU3054 and DAPG could be used a potential bio inoculants for the management of bacterial wilt disease of tomato.
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Claims 10
Claim · model-written summary
Cell-free culture filtrate of P. fluorescens VSMKU3054 significantly arrested growth of Ralstonia solanacearum compared with control.
- Treatment
- cell free culture filtrate of P. fluorescens VSMKU3054e0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Second interactor
- P. fluorescens VSMKU3054
.JPG?width=60)
Pseudomonas fluorescense0biocontrol_agent - Outcome
- growthe0
- Direction
- arrestede0
- Comparator
- controle0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“The cell free culture filtrate of P. fluorescens VSMKU3054 was significantly arrested the growth of R. solanacearum and fungal pathogens such as Rhizoctonia solani, Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporum compared to control”
Claim · model-written summary
Cell-free culture filtrate of P. fluorescens VSMKU3054 significantly arrested growth of the fungal pathogens Rhizoctonia solani, Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporum compared with control.
- Treatment
- cell free culture filtrate of P. fluorescens VSMKU3054e0
- Interactor
- fungal pathogense0pathogen
- Second interactor
- P. fluorescens VSMKU3054Pseudomonas fluorescense0biocontrol_agent
- Outcome
- growthe0
- Direction
- arrestede0
- Qualifier
- Rhizoctonia solani, Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporume0
- Comparator
- controle0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell free culture filtrate of P. fluorescens VSMKU3054 was significantly arrested the growth of R. solanacearum and fungal pathogens such as Rhizoctonia solani, Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporum compared to control”
Claim · model-written summary
The presence of DAPG in the crude metabolites of P. fluorescens VSMKU3054 was confirmed on TLC with an Rf value of 0.34.
Not reportedTaxonDirection
Verbatim
e0“The existence of DAPG from the crude metabolites of P. fluorescens VSMKU3054 was confirmed on TLC with Rf value 0.34”
Claim · model-written summary
Partially purified DAPG at 30 ug/ml showed much higher activity against R. solanacearum than against the fungal plant pathogens, compared with control.
- Treatment
- partially purified DAPGe0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Second interactor
- fungal plant pathogense0pathogen
- Outcome
- activity against R. solanacearume0
- Direction
- much highere0
- Qualifier
- at 30 µg/mle0
- Comparator
- controle0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The partially purified DAPG exhibited much higher activity against R. solanacearum at 30 µg/ml than the fungal plant pathogens compared to control”
Claim · model-written summary
The antimicrobial partially purified compound was identified as DAPG by UV, FT-IR and GC-MS analysis.
- Outcome
- identified as DAPGe0
Not reportedTaxonDirection
Verbatim
e0“The antimicrobial partially purified compound was identified as DAPG”
Claim · model-written summary
DAPG at 30 ug/ml significantly controlled the living nature of R. solanacearum by up to 68% compared with tetracycline and universal control.
- Treatment
- DAPG at 30 µg/mle0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Outcome
- living nature of R. solanacearume0
- Direction
- controllede0
- Value
- 68%e0
- Comparator
- tetracycline and universal controle0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of live cells of R. solanacearum when supplemented with DAPG at 30 µg/ml, significantly controlled the living nature of R. solanacearum up to 68% compared to tetracycline and universal control”
Claim · model-written summary
The selected isolate P. fluorescens VSMKU3054 significantly controlled wilt disease of tomato by up to 59.5% compared with positive and negative control in a detached leaf assay.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- The selected isolate P. fluorescens VSMKU3054e0
- Interactor
- P. fluorescens VSMKU3054Pseudomonas fluorescense0biocontrol_agent
- Outcome
- wilt diseasee0
- Direction
- controllede0
- Value
- 59.5%e0
- Qualifier
- on 3rd and 7th dayse0
- Comparator
- positive and negative controle0
- Setting
- detached leaf assaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The selected isolate P. fluorescens VSMKU3054 and DAPG significantly controlled wilt disease of tomato up to 59.5% and 42.12% on 3rd and 7th days compared to positive and negative control by detached leaf assay”
Claim · model-written summary
DAPG significantly controlled wilt disease of tomato by up to 42.12% compared with positive and negative control in a detached leaf assay.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- DAPGe0
- Outcome
- wilt diseasee0
- Direction
- controllede0
- Value
- 42.12%e0
- Qualifier
- on 3rd and 7th dayse0
- Comparator
- positive and negative controle0
- Setting
- detached leaf assaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The selected isolate P. fluorescens VSMKU3054 and DAPG significantly controlled wilt disease of tomato up to 59.5% and 42.12% on 3rd and 7th days compared to positive and negative control by detached leaf assay”
Claim · model-written summary
In silico analysis showed high interaction of the DAPG-encoding protease with a lectin associated with R. solanacearum.
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Gene
- lectine0
- Outcome
- interactione0
- Direction
- highe0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in silico analysis revealed that high interaction of DAPG encoding protease with lectin which is associated with R. solanacearum”
Claim · model-written summary
The authors conclude that P. fluorescens VSMKU3054 and DAPG could be used as potential bio-inoculants for managing bacterial wilt disease of tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- management of bacterial wilt diseasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. fluorescens VSMKU3054 and DAPG could be used a potential bio inoculants for the management of bacterial wilt disease of tomato”
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