Abstract
Ralstonia solanacearum (Rs, biovar2, race3) is the causal agent of bacterial wilt and this quarantine phytopathogen is responsible for massive loss in several commercially important crops. Biological control of this pathogen is a suitable solution in areas where Rs is endemic. We tested 12 in vitro-selected bacterial antagonists of Rs displaying efficient biological control of wilt symptoms on tomato grown under greenhouse conditions. The population densities of Rs and antagonists were estimated by an integrated strategy coupling selective plating, real-time PCR and fliC gene PCR-Southern blot hybridization in total rhizosphere community DNA. Moreover, we focused on how the Rs-antagonist complex altered the composition of the tomato rhizosphere microbiota via 16S rRNA gene amplicon sequencing. Amongst tested antagonists, Bacillus velezensis (B63) and Pseudomonas fluorescens (P142)-inoculated plants were clean of wilt disease symptoms, accompanied with significant decrease in Rs population compared to non-inoculated controls. The effective biocontrol of Rs populations by antagonists resulted in pronounced shifts in bacterial community compositions, highlighting dynamic taxa responding to Rs and/or inoculants presence and indicating that bacterial competitive niche exclusion and faciliation processes are likely at play. This is supported by confocal laser scanning microscopy visualization of antagonist P-142 colonization patterns via gfp-tagged cells, revealing establishment in lateral roots, root hairs, epidermal cells and within xylem vessels. Both inoculants are promising biological agents for efficient control Rs under field conditions.
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Claims 7
Claim · model-written summary
Bacillus velezensis (B63) inoculation left tomato plants free of wilt symptoms.
- Taxon
- Tomato Plant
Solanum lycopersicumt0 - Treatment
- Bacillus velezensis (B63)e0
- Interactor
- Rse0pathogen
- Second interactor
- Bacillus velezensis (B63)
Bacillus velezensise0biocontrol_agent - Outcome
- clean of wilt disease symptomse0
- Comparator
- non-inoculated controlse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amongst tested antagonists, Bacillus velezensis (B63) and Pseudomonas fluorescens (P142)-inoculated plants were clean of wilt disease symptoms, accompanied with significant decrease in Rs population compared to non-inoculated controls”
t0“Tomato Plant”
Claim · model-written summary
Bacillus velezensis (B63) inoculation significantly decreased rhizosphere Rs population relative to non-inoculated controls.
- Taxon
- Tomato PlantSolanum lycopersicumt0
- Treatment
- Bacillus velezensis (B63)e0
- Interactor
- Rse0pathogen
- Second interactor
- Bacillus velezensis (B63)Bacillus velezensise0biocontrol_agent
- Outcome
- Rs populatione0
- Direction
- decreasee0
- Comparator
- non-inoculated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amongst tested antagonists, Bacillus velezensis (B63) and Pseudomonas fluorescens (P142)-inoculated plants were clean of wilt disease symptoms, accompanied with significant decrease in Rs population compared to non-inoculated controls”
t0“Tomato Plant”
Claim · model-written summary
Pseudomonas fluorescens (P142) inoculation left tomato plants free of wilt symptoms.
- Taxon
- Tomato PlantSolanum lycopersicumt0
- Treatment
- Pseudomonas fluorescens (P142)e0
- Interactor
- Rse0pathogen
- Second interactor
- Pseudomonas fluorescens (P142)
.JPG?width=60)
Pseudomonas fluorescense0biocontrol_agent - Outcome
- clean of wilt disease symptomse0
- Comparator
- non-inoculated controlse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“Amongst tested antagonists, Bacillus velezensis (B63) and Pseudomonas fluorescens (P142)-inoculated plants were clean of wilt disease symptoms, accompanied with significant decrease in Rs population compared to non-inoculated controls”
t0“Tomato Plant”
Claim · model-written summary
Pseudomonas fluorescens (P142) inoculation significantly decreased rhizosphere Rs population relative to non-inoculated controls.
- Taxon
- Tomato PlantSolanum lycopersicumt0
- Treatment
- Pseudomonas fluorescens (P142)e0
- Interactor
- Rse0pathogen
- Second interactor
- Pseudomonas fluorescens (P142)Pseudomonas fluorescense0biocontrol_agent
- Outcome
- Rs populatione0
- Direction
- decreasee0
- Comparator
- non-inoculated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amongst tested antagonists, Bacillus velezensis (B63) and Pseudomonas fluorescens (P142)-inoculated plants were clean of wilt disease symptoms, accompanied with significant decrease in Rs population compared to non-inoculated controls”
t0“Tomato Plant”
Claim · model-written summary
Antagonist control of Rs shifted rhizosphere bacterial community composition.
- Taxon
- Tomato PlantSolanum lycopersicumt0
- Treatment
- antagonistse0
- Interactor
- Rse0pathogen
- Outcome
- bacterial community compositionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effective biocontrol of Rs populations by antagonists resulted in pronounced shifts in bacterial community compositions”
t0“Tomato Plant”
Claim · model-written summary
P-142 colonized lateral roots, root hairs, epidermal cells and xylem vessels.
- Taxon
- Tomato PlantSolanum lycopersicumt0
- Interactor
- P-142e0biocontrol_agent
- Outcome
- establishment in lateral roots, root hairs, epidermal cells and within xylem vesselse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antagonist P-142 colonization patterns via gfp-tagged cells, revealing establishment in lateral roots, root hairs, epidermal cells and within xylem vessels”
t0“Tomato Plant”
Claim · model-written summary
The authors consider both inoculants promising field biocontrol agents against Rs.
- Taxon
- Tomato PlantSolanum lycopersicumt0
- Interactor
- Rse0pathogen
- Outcome
- efficient control Rs under field conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both inoculants are promising biological agents for efficient control Rs under field conditions”
t0“Tomato Plant”
The same questions in other papers
Bacillus velezensis · pest · Solanum lycopersicum (tomato)Results differ
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Pseudomonas fluorescens · pest · Solanum lycopersicum (tomato)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- no effect
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See all 11 papers →Pseudomonas fluorescens · disease · Solanum lycopersicum (tomato)Positive and negative results
18 papers in the same direction as this paper · 1 in the opposite direction
- positive
- positive
- no effect
16 papers more in this group
See all 20 papers →Bacillus velezensis · disease · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.