Abstract
Abstract Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants by delivering a repertoire of type III effectors. In the present study, we determined that the deletion of the type III effector RipAF1 resulted in increased virulence on Nicotiana benthamiana, Solanum lycopersicum, and Capsicum annuum plants. RipAF1 showed ADP-ribosylation activity in vivo and in vitro. Transient overexpression of RipAF1 suppressed jasmonic acid (JA) signaling and induced salicylic acid (SA) signaling. The ADP-ribosylation activity of RipAF1 was essential for JA and SA signaling mediation. Host fibrillin FBN1 was identified as a RipAF1-interactor that is ADP-ribosylated by RipAF1 directly. Most importantly, the ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane and leads to the suppression of JA signaling and induction of SA signaling. We concluded that RipAF1 mediates antagonistic crosstalk between JA and SA signaling pathways by ADP-ribosylation of FBN1.
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Claims 13
Claim · model-written summary
Deletion of RipAF1 increased Ralstonia solanacearum virulence on Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- deletion of the type III effector RipAF1e1
- Interactor
- Ralstonia solanacearum
Ralstonia solanacearume0pathogen - Gene
- RipAF1e1
- Outcome
- virulencee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e1“the deletion of the type III effector RipAF1 resulted in increased virulence on Nicotiana benthamiana, Solanum lycopersicum, and Capsicum annuum plants”
Claim · model-written summary
Deletion of RipAF1 increased Ralstonia solanacearum virulence on Solanum lycopersicum.
- Taxon
- Solanum lycopersicum
Solanum lycopersicume1 - Treatment
- deletion of the type III effector RipAF1e1
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- RipAF1e1
- Outcome
- virulencee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e1“the deletion of the type III effector RipAF1 resulted in increased virulence on Nicotiana benthamiana, Solanum lycopersicum, and Capsicum annuum plants”
Claim · model-written summary
Deletion of RipAF1 increased Ralstonia solanacearum virulence on Capsicum annuum.
- Taxon
- Capsicum annuum
Capsicum annuume1 - Treatment
- deletion of the type III effector RipAF1e1
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- RipAF1e1
- Outcome
- virulencee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e1“the deletion of the type III effector RipAF1 resulted in increased virulence on Nicotiana benthamiana, Solanum lycopersicum, and Capsicum annuum plants”
Claim · model-written summary
RipAF1 showed ADP-ribosylation activity in vivo and in vitro.
- Taxon
- host plantse0
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- RipAF1e2
- Outcome
- ADP-ribosylation activitye2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e2“RipAF1 showed ADP-ribosylation activity in vivo and in vitro”
Claim · model-written summary
Transient overexpression of RipAF1 suppressed jasmonic acid signaling in host plants.
- Taxon
- host plantse0
- Treatment
- Transient overexpression of RipAF1e3
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- RipAF1e3
- Compound
- jasmonic acide3
- Outcome
- jasmonic acid (JA) signalinge3
- Direction
- suppressede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e3“Transient overexpression of RipAF1 suppressed jasmonic acid (JA) signaling and induced salicylic acid (SA) signaling”
Claim · model-written summary
Transient overexpression of RipAF1 induced salicylic acid signaling in host plants.
- Taxon
- host plantse0
- Treatment
- Transient overexpression of RipAF1e3
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- RipAF1e3
- Compound
- salicylic acide3
- Outcome
- salicylic acid (SA) signalinge3
- Direction
- inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e3“Transient overexpression of RipAF1 suppressed jasmonic acid (JA) signaling and induced salicylic acid (SA) signaling”
Claim · model-written summary
The ADP-ribosylation activity of RipAF1 is essential for mediating JA and SA signaling.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e4“The ADP-ribosylation activity of RipAF1 was essential for JA and SA signaling mediation”
Claim · model-written summary
Host fibrillin FBN1 was identified as a RipAF1-interacting protein.
- Taxon
- host plantse0
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- FBN1e5
- Outcome
- RipAF1-interactore5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e5“Host fibrillin FBN1 was identified as a RipAF1-interactor that is ADP-ribosylated by RipAF1 directly”
Claim · model-written summary
RipAF1 directly ADP-ribosylates host FBN1.
- Taxon
- host plantse0
- Interactor
- Ralstonia solanacearumRalstonia solanacearume0pathogen
- Gene
- FBN1e5
- Outcome
- ADP-ribosylatede5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e5“Host fibrillin FBN1 was identified as a RipAF1-interactor that is ADP-ribosylated by RipAF1 directly”
Claim · model-written summary
ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e6“the ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane and leads to the suppression of JA signaling and induction of SA signaling”
Claim · model-written summary
ADP-ribosylation of conserved residues of FBN1 leads to suppression of JA signaling.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e6“the ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane and leads to the suppression of JA signaling and induction of SA signaling”
Claim · model-written summary
ADP-ribosylation of conserved residues of FBN1 leads to induction of SA signaling.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e6“the ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane and leads to the suppression of JA signaling and induction of SA signaling”
Claim · model-written summary
RipAF1 mediates antagonistic crosstalk between JA and SA signaling by ADP-ribosylation of FBN1.
Verbatim
e0“Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants”
e7“We concluded that RipAF1 mediates antagonistic crosstalk between JA and SA signaling pathways by ADP-ribosylation of FBN1”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.