Abstract
The protocol has been developed and standardized in two date palm (Phoenix dactylifera L.) cultivars. Protocol was cultivar and plant growth regulators dependant. Embryogenic callus from excised shoot tips was induced on MS medium supplemented with 2,4-D 100 mg/l. A combination of ά-naphthalene acetic acid and 6-benzyladenine (NAA 0.1 + BA 0.05) in MS medium multiplied callus 3-4 times followed by abscisic acid (ABA, 0.5 mg/l) treatment matures callus and accelerated the initiation of somatic embryos in both the cultivars. Treatment combination of abscisic acid (0.5 mg/l) + indole-3-butyric acid (IBA, 0.05 mg/l) in medium found effective in maturation of somatic embryos. Somatic embryos germinated and converted into plantlets in half strength modified MS medium containing different concentrations of IBA @ 0.1, 0.2, 0.3 mg/l and NAA @ 0.1, 0.2, 0.3 mg/l. At a higher concentration (0.3 mg/l), in vitro roots and leaves (plantlets) were developed while at lower concentrations, response was comparatively poor. Secondary hardened plants after primary hardening were transplanted in the field for assessing their field performance. The findings of this paper indicate that enhanced production of somatic embryos and their conversion into plantlets can be a potential source of alternative mass propagation of date palm.
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Claims 7
Claim · model-written summary
The propagation protocol depended on cultivar and growth regulators.
- Taxon
- date palm
Phoenix dactyliferat0 - Outcome
- Protocol was cultivar and plant growth regulators dependante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“Protocol was cultivar and plant growth regulators dependant.”
Claim · model-written summary
MS medium with 2,4-D induced embryogenic callus from shoot tips.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- excised shoot tipse0
- Treatment
- MS medium supplemented with 2,4-D 100 mg/le0
- Outcome
- Embryogenic calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“Embryogenic callus from excised shoot tips was induced on MS medium supplemented with 2,4-D 100 mg/l.”
Claim · model-written summary
NAA plus BA multiplied callus 3-4 times.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- NAA 0.1 + BA 0.05e0
- Outcome
- calluse0
- Direction
- multipliede0
- Value
- 3-4 timese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“A combination of ά-naphthalene acetic acid and 6-benzyladenine (NAA 0.1 + BA 0.05) in MS medium multiplied callus 3-4 times”
Claim · model-written summary
ABA treatment matured callus and accelerated somatic embryo initiation.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- abscisic acid (ABA, 0.5 mg/l) treatmente0
- Outcome
- initiation of somatic embryose0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“abscisic acid (ABA, 0.5 mg/l) treatment matures callus and accelerated the initiation of somatic embryos in both the cultivars.”
Claim · model-written summary
ABA and IBA medium was effective for somatic embryo maturation.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- abscisic acid (0.5 mg/l) + indole-3-butyric acid (IBA, 0.05 mg/l)e0
- Outcome
- maturation of somatic embryose0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“Treatment combination of abscisic acid (0.5 mg/l) + indole-3-butyric acid (IBA, 0.05 mg/l) in medium found effective in maturation of somatic embryos.”
Claim · model-written summary
Somatic embryos germinated and formed plantlets in half strength MS medium with IBA and NAA.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- half strength modified MS medium containing different concentrations of IBA @ 0.1, 0.2, 0.3 mg/l and NAA @ 0.1, 0.2, 0.3 mg/le0
- Outcome
- Somatic embryos germinated and converted into plantletse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“Somatic embryos germinated and converted into plantlets in half strength modified MS medium containing different concentrations of IBA @ 0.1, 0.2, 0.3 mg/l and NAA @ 0.1, 0.2, 0.3 mg/l.”
Claim · model-written summary
At 0.3 mg/l, plantlet roots and leaves developed, versus poorer responses at lower concentrations.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- higher concentration (0.3 mg/l)e0
- Outcome
- in vitro roots and leaves (plantlets)e0
- Comparator
- lower concentrationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm (Phoenix dactylifera L.)”
e0“At a higher concentration (0.3 mg/l), in vitro roots and leaves (plantlets) were developed while at lower concentrations, response was comparatively poor.”
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See all 51 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.