Abstract
BACKGROUND: Date palm (Phoenix dactylifera L.) is a traditional crop in arid and semi-arid areas. Its vegetative propagation can be achieved by offshoots, but possible number of offshoots in mother palm trees is limited. Micropropagation is a highly recommended strategy for obtaining date palm elite cultivars using shoot tip and immature inflorescences. In this study, micropropagation procedure using inflorescence explants of Medjool cv. is described. For culture initiation, explants from different spathe lengths were cultivated on Murashige and Skoog medium (MS) supplemented with picloram at 1.0 and 2.0 mg/l combined with 2iP at 0.5 mg/l alone and with both 2iP and BA at 0.25 mg/l for 24 weeks. The obtained direct globular embryos were transferred to maturation media with 0.1 mg/l picloram alone or combined with both 2iP and ABA separately and together for further development. Additionally, multiplication and rooting media were optimized by different cytokinins and auxins for high frequency of plantlet production. Acclimatization of in vitro plantlets was also investigated. RESULTS: The highest percentage of globular embryo formation was noticed with explants isolated from spathe lengths ranging from 10 to 15 cm. Addition of BA to initiation media with picloram encouraged a significant effect on embryonic culture formation percentage. Incorporation of ABA and 2iP to maturation medium was an effective factor for individual or multiple embryo emergence. Acclimatization of in vitro plantlets having 3-4 roots was successfully accomplished. Irrigation with the full strength solution (MS) encouraged the highest growth vigor degree, leaf number/plant, leaf width, root number, and root thickness degree of ex vitro plants. CONCLUSION: This research provides an advanced regeneration system for large-scale production of date palm from immature inflorescences of Medjool cv. It opens up the prospects of using picloram with different growth regulators for rapid micropropagation of date palm.
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Claims 6
Claim · model-written summary
Spathe lengths of 10 to 15 cm gave the highest globular embryo formation.
- Taxon
- Date palm
Phoenix dactyliferat0 - Material
- Medjool cv.e1
- Organ / stage
- spathee0
- Outcome
- percentage of globular embryo formatione0
- Direction
- higheste0
- Value
- 10 to 15 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of globular embryo formation was noticed with explants isolated from spathe lengths ranging from 10 to 15 cm.”
t0“Date palm”
e1“inflorescence explants of Medjool cv.”
Claim · model-written summary
Adding BA significantly improved embryonic culture formation percentage.
- Taxon
- Date palmPhoenix dactyliferat0
- Treatment
- Addition of BA to initiation media with piclorame0
- Outcome
- embryonic culture formation percentagee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of BA to initiation media with picloram encouraged a significant effect on embryonic culture formation percentage.”
t0“Date palm”
Claim · model-written summary
ABA and 2iP in maturation medium promoted embryo emergence.
- Taxon
- Date palmPhoenix dactyliferat0
- Treatment
- Incorporation of ABA and 2iP to maturation mediume0
- Outcome
- individual or multiple embryo emergencee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incorporation of ABA and 2iP to maturation medium was an effective factor for individual or multiple embryo emergence.”
t0“Date palm”
Claim · model-written summary
Plantlets with 3-4 roots were successfully acclimatized.
- Taxon
- Date palmPhoenix dactyliferat0
- Organ / stage
- plantletse0
- Outcome
- Acclimatization of in vitro plantletse0
- Qualifier
- 3-4 rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acclimatization of in vitro plantlets having 3-4 roots was successfully accomplished.”
t0“Date palm”
Claim · model-written summary
Full-strength MS irrigation gave the highest growth vigor, leaf number and width.
- Taxon
- Date palmPhoenix dactyliferat0
- Treatment
- Irrigation with the full strength solution (MS)e0
- Outcome
- growth vigor degree, leaf number/plant, leaf widthe0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irrigation with the full strength solution (MS) encouraged the highest growth vigor degree, leaf number/plant, leaf width, root number, and root thickness degree of ex vitro plants.”
t0“Date palm”
Claim · model-written summary
Full-strength MS irrigation gave the highest root number and thickness.
- Taxon
- Date palmPhoenix dactyliferat0
- Treatment
- Irrigation with the full strength solution (MS)e0
- Outcome
- root number, and root thickness degreee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irrigation with the full strength solution (MS) encouraged the highest growth vigor degree, leaf number/plant, leaf width, root number, and root thickness degree of ex vitro plants.”
t0“Date palm”
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