Paper · 1978 · Physiologia Plantarum

Effects of Organic Nutrients and Hormones on Growth and Development of Tissue Explants from Coconut (Cocos nucifera) and Date (Phoenix dactylifera) Palms Cultured in vitro

C. J. EEUWENS

7
claims
12
verbatim quotes
77
citations
4
research areas
—
Not open access

Abstract

Abstract The growth response (increase in weight) of cultured explants from seedling date ( Phoenix dactylifera L.) and mature coconut ( Cocos nucifera L. cv. Malayan Dwarf) palms to source and concentration of organic nitrogen. carbohydrate, auxins, cytokinins and gibberellins was examined. Growth was strongly stimulated by the presence of auxins (10 −7 to 10 −6 M ), cytokinins (10 −6 to 10 −5 M ), high concentrations of sucrose (0.2 M ), and in the absence of NH 4 Cl, by organic sources of reduced nitrogen. Higher concentrations of auxin (2,4‐D or NAA at 10 −6 to 10 −5 M ) which still stimulated growth of Phoenix tissue, proved inhibitory to growth of freshly excised Cocos tissues. Explants from both palms initiated roots when subcultured on a medium with increased levels of auxin (NAA, 2.5 × 10 −6 to 2.5 × 10 −5 M ) and reduced levels of cytokinin (6‐BAP, 5 × 10 −8 M ). Isolated roots excised from these explants continued growth and produced new laterals when subcultured on media with GA 3 (5 × 10 −7 M ) and reduced levels of auxin, cytokinin, and either minerals or sucrose.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Phoenix dactylifera L. explant growth was stimulated by auxins, cytokinins, sucrose and organic reduced nitrogen.

Taxon
Phoenix dactylifera L.Phoenix dactyliferae1
Treatment
auxins (10 −7 to 10 −6 M )e0
Outcome
Growthe0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Growth was strongly stimulated by the presence of auxins (10 −7 to 10 −6 M ), cytokinins (10 −6 to 10 −5 M ), high concentrations of sucrose (0.2 M ), and in the absence of NH 4 Cl, by organic sources of reduced nitrogen”

✓ located verbatim in the source · abstract, characters 293–513 · show in text ↑
e1

“seedling date ( Phoenix dactylifera L.)”

✓ located verbatim in the source · abstract, characters 76–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cocos nucifera L. cv. Malayan Dwarf explant growth was stimulated by auxins, cytokinins, sucrose and organic reduced nitrogen.

Taxon
Cocos nucifera L. cv. Malayan DwarfCocos nuciferae1
Treatment
auxins (10 −7 to 10 −6 M )e0
Outcome
Growthe0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cocos nucifera
Cocos nucifera · Taxon · picture from its Wikimedia Commons categoryThamizhpparithi Maari · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Growth was strongly stimulated by the presence of auxins (10 −7 to 10 −6 M ), cytokinins (10 −6 to 10 −5 M ), high concentrations of sucrose (0.2 M ), and in the absence of NH 4 Cl, by organic sources of reduced nitrogen”

✓ located verbatim in the source · abstract, characters 293–513 · show in text ↑
e1

“mature coconut ( Cocos nucifera L. cv. Malayan Dwarf)”

✓ located verbatim in the source · abstract, characters 120–173 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Higher auxin concentrations still stimulated Phoenix tissue growth.

Taxon
PhoenixPhoenixe0
Treatment
auxin (2,4‐D or NAA at 10 −6 to 10 −5 M )e0
Outcome
growth of Phoenix tissuee0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix
Phoenix · Taxon · picture of the genus (one of its species)Mmcknight4 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Higher concentrations of auxin (2,4‐D or NAA at 10 −6 to 10 −5 M ) which still stimulated growth of Phoenix tissue”

✓ located verbatim in the source · abstract, characters 515–629 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Higher auxin concentrations inhibited growth of freshly excised Cocos tissues.

Taxon
CocosCocose0
Treatment
auxin (2,4‐D or NAA at 10 −6 to 10 −5 M )e0
Outcome
growth of freshly excised Cocos tissuese0
Direction
inhibitorye0

Not reportedComparator

Verbatim

e0

“Higher concentrations of auxin (2,4‐D or NAA at 10 −6 to 10 −5 M ) which still stimulated growth of Phoenix tissue, proved inhibitory to growth of freshly excised Cocos tissues”

✓ located verbatim in the source · abstract, characters 515–691 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Phoenix dactylifera L. explants initiated roots on increased auxin and reduced cytokinin medium.

Taxon
Phoenix dactylifera L.Phoenix dactyliferae1
Treatment
increased levels of auxin (NAA, 2.5 × 10 −6 to 2.5 × 10 −5 M ) and reduced levels of cytokinin (6‐BAP, 5 × 10 −8 M )e0
Outcome
initiated rootse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Explants from both palms initiated roots when subcultured on a medium with increased levels of auxin (NAA, 2.5 × 10 −6 to 2.5 × 10 −5 M ) and reduced levels of cytokinin (6‐BAP, 5 × 10 −8 M )”

✓ located verbatim in the source · abstract, characters 693–884 · show in text ↑
e1

“seedling date ( Phoenix dactylifera L.)”

✓ located verbatim in the source · abstract, characters 76–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cocos nucifera L. cv. Malayan Dwarf explants initiated roots on increased auxin and reduced cytokinin medium.

Taxon
Cocos nucifera L. cv. Malayan DwarfCocos nuciferae1
Treatment
increased levels of auxin (NAA, 2.5 × 10 −6 to 2.5 × 10 −5 M ) and reduced levels of cytokinin (6‐BAP, 5 × 10 −8 M )e0
Outcome
initiated rootse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cocos nucifera
Cocos nucifera · Taxon · picture from its Wikimedia Commons categoryThamizhpparithi Maari · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Explants from both palms initiated roots when subcultured on a medium with increased levels of auxin (NAA, 2.5 × 10 −6 to 2.5 × 10 −5 M ) and reduced levels of cytokinin (6‐BAP, 5 × 10 −8 M )”

✓ located verbatim in the source · abstract, characters 693–884 · show in text ↑
e1

“mature coconut ( Cocos nucifera L. cv. Malayan Dwarf)”

✓ located verbatim in the source · abstract, characters 120–173 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Isolated roots continued growth and produced new laterals on GA3 medium with reduced auxin, cytokinin and minerals or sucrose.

Taxon
Phoenix dactylifera L.Phoenix dactyliferae1
Organ / stage
Isolated rootse0
Treatment
media with GA 3 (5 × 10 −7 M ) and reduced levels of auxin, cytokinin, and either minerals or sucrosee0
Outcome
continued growth and produced new lateralse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Isolated roots excised from these explants continued growth and produced new laterals when subcultured on media with GA 3 (5 × 10 −7 M ) and reduced levels of auxin, cytokinin, and either minerals or sucrose”

✓ located verbatim in the source · abstract, characters 886–1093 · show in text ↑
e1

“cultured explants from seedling date ( Phoenix dactylifera L.) and mature coconut ( Cocos nucifera L. cv. Malayan Dwarf) palms”

✓ located verbatim in the source · abstract, characters 53–179 · show in text ↑

Gibberellic acid · growth · Phoenix dactylifera (date palm)Results agree

10 papers: 10 positive, 0 negative or no effect

9 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

sucrose · growth · Phoenix dactylifera (date palm)Results agree

20 papers: 20 positive, 0 negative or no effect

19 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

class · growth · Cocos nucifera (coconut palm)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

class · growth · Phoenix dactylifera (date palm)Positive and negative results

19 papers: 14 positive, 5 negative or no effect

13 papers in the same direction as this paper · 5 in the opposite direction

  1. negative
  2. negative
  3. no effect

15 papers more in this group

See all 19 papers →

in vitro · growth · Phoenix dactylifera (date palm)Positive and negative results

51 papers: 50 positive, 1 negative or no effect

49 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

47 papers more in this group

See all 51 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.