Abstract
BACKGROUND: Despite increasing characterization of DEAD-box RNA helicases (RHs) in chloroplast gene expression regulation at posttranscriptional levels in plants, their functional roles in growth responses of crops, including rice (Oryza sativa), to abiotic stresses are yet to be characterized. In this study, rice OsRH58 (LOC_Os01g73900), a chloroplast-localized DEAD-box RH, was characterized for its expression patterns upon stress treatment and its functional roles using transgenic Arabidopsis plants under normal and abiotic stress conditions. RESULTS: Chloroplast localization of OsRH58 was confirmed by analyzing the expression of OsRH58-GFP fusion proteins in tobacco leaves. Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress, whereas its expression was decreased by cold, UV, or ABA treatment. The OsRH58-expressing Arabidopsis plants were taller and had more seeds than the wild type under favorable conditions. The transgenic plants displayed faster seed germination, better seedling growth, and a higher survival rate than the wild type under high salt or drought stress. Importantly, levels of several chloroplast proteins were increased in the transgenic plants under salt or dehydration stress. Notably, OsRH58 harbored RNA chaperone activity. CONCLUSIONS: These findings suggest that the chloroplast-transported OsRH58 possessing RNA chaperone activity confers stress tolerance by increasing translation of chloroplast mRNAs.
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Claims 15
Claim · model-written summary
OsRH58 localized to chloroplasts in tobacco leaves.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leavese0
- Gene
- OsRH58e0
- Outcome
- Chloroplast localizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chloroplast localization of OsRH58 was confirmed by analyzing the expression of OsRH58-GFP fusion proteins in tobacco leaves”
Claim · model-written summary
Salt stress upregulated OsRH58 expression in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- salte0
- Gene
- OsRH58e0
- Outcome
- Expression of OsRH58e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress”
Claim · model-written summary
Drought stress upregulated OsRH58 expression in rice.
- Taxon
- riceOryza sativae0
- Treatment
- droughte0
- Gene
- OsRH58e0
- Outcome
- Expression of OsRH58e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress”
Claim · model-written summary
Heat stress upregulated OsRH58 expression in rice.
- Taxon
- riceOryza sativae0
- Treatment
- heat stresse0
- Gene
- OsRH58e0
- Outcome
- Expression of OsRH58e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress”
Claim · model-written summary
Cold treatment decreased OsRH58 expression in rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress, whereas its expression was decreased by cold, UV, or ABA treatment”
Claim · model-written summary
UV treatment decreased OsRH58 expression in rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress, whereas its expression was decreased by cold, UV, or ABA treatment”
Claim · model-written summary
ABA treatment decreased OsRH58 expression in rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsRH58 in rice was up-regulated by salt, drought, or heat stress, whereas its expression was decreased by cold, UV, or ABA treatment”
Claim · model-written summary
OsRH58-expressing Arabidopsis plants were taller than wild type under favorable conditions.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- OsRH58-expressinge0
- Gene
- OsRH58e0
- Outcome
- tallere0
- Direction
- tallere0
- Qualifier
- favorable conditionse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsRH58-expressing Arabidopsis plants were taller and had more seeds than the wild type under favorable conditions”
Claim · model-written summary
OsRH58-expressing Arabidopsis plants had more seeds than wild type under favorable conditions.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- OsRH58-expressinge0
- Gene
- OsRH58e0
- Outcome
- seedse0
- Direction
- moree0
- Qualifier
- favorable conditionse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsRH58-expressing Arabidopsis plants were taller and had more seeds than the wild type under favorable conditions”
Claim · model-written summary
Transgenic Arabidopsis had faster germination, better seedling growth and higher survival under high salt stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenic plantse0
- Outcome
- seed germination, better seedling growth, and a higher survival ratee0
- Direction
- highere0
- Qualifier
- high salte0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants displayed faster seed germination, better seedling growth, and a higher survival rate than the wild type under high salt or drought stress”
e1“transgenic Arabidopsis plants”
Claim · model-written summary
Transgenic Arabidopsis had faster germination, better seedling growth and higher survival under drought stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenic plantse0
- Outcome
- seed germination, better seedling growth, and a higher survival ratee0
- Direction
- highere0
- Qualifier
- drought stresse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants displayed faster seed germination, better seedling growth, and a higher survival rate than the wild type under high salt or drought stress”
e1“transgenic Arabidopsis plants”
Claim · model-written summary
Several chloroplast protein levels increased in transgenic plants under salt stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenic plantse0
- Outcome
- levels of several chloroplast proteinse0
- Direction
- increasede0
- Qualifier
- salte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“levels of several chloroplast proteins were increased in the transgenic plants under salt or dehydration stress”
e1“transgenic Arabidopsis plants”
Claim · model-written summary
Several chloroplast protein levels increased in transgenic plants under dehydration stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenic plantse0
- Outcome
- levels of several chloroplast proteinse0
- Direction
- increasede0
- Qualifier
- dehydration stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“levels of several chloroplast proteins were increased in the transgenic plants under salt or dehydration stress”
e1“transgenic Arabidopsis plants”
Claim · model-written summary
OsRH58 had RNA chaperone activity.
- Taxon
- riceOryza sativae1
- Gene
- OsRH58e0
- Outcome
- RNA chaperone activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRH58 harbored RNA chaperone activity”
e1“rice OsRH58”
Claim · model-written summary
The authors propose that OsRH58 confers stress tolerance by increasing chloroplast mRNA translation.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- OsRH58e0
- Outcome
- stress tolerancee0
- Qualifier
- increasing translation of chloroplast mRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chloroplast-transported OsRH58 possessing RNA chaperone activity confers stress tolerance by increasing translation of chloroplast mRNAs”
e1“transgenic Arabidopsis plants”
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