Abstract
The NAC transcription factors play critical roles in regulating stress responses in plants. However, the functions for many of the NAC family members in rice are yet to be identified. In the present study, a novel stress-responsive rice NAC gene, ONAC022, was identified. Expression of ONAC022 was induced by drought, high salinity, and abscisic acid (ABA). The ONAC022 protein was found to bind specifically to a canonical NAC recognition cis-element sequence and showed transactivation activity at its C-terminus in yeast. The ONAC022 protein was localized to nucleus when transiently expressed in Nicotiana benthamiana. Three independent transgenic rice lines with overexpression of ONAC022 were generated and used to explore the function of ONAC022 in drought and salt stress tolerance. Under drought stress condition in greenhouse, soil-grown ONAC022-overexpressing (N22oe) transgenic rice plants showed an increased drought tolerance, leading to higher survival ratios and better growth than wild-type (WT) plants. When grown hydroponically in Hogland solution supplemented with 150 mM NaCl, the N22oe plants displayed an enhanced salt tolerance and accumulated less Na(+) in roots and shoots as compared to WT plants. Under drought stress condition, the N22oe plants exhibited decreased rates of water loss and transpiration, reduced percentage of open stomata and increased contents of proline and soluble sugars. However, the N22oe lines showed increased sensitivity to exogenous ABA at seed germination and seedling growth stages but contained higher level of endogenous ABA. Expression of some ABA biosynthetic genes (OsNCEDs and OsPSY), signaling and regulatory genes (OsPP2C02, OsPP2C49, OsPP2C68, OsbZIP23, OsAP37, OsDREB2a, and OsMYB2), and late stress-responsive genes (OsRAB21, OsLEA3, and OsP5CS1) was upregulated in N22oe plants. Our data demonstrate that ONAC022 functions as a stress-responsive NAC with transcriptional activator activity and plays a positive role in drought and salt stress tolerance through modulating an ABA-mediated pathway.
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Claims 14
Claim · model-written summary
A novel stress-responsive rice NAC gene ONAC022 was identified.
- Taxon
- rice
Oryza sativae0 - Gene
- ONAC022e0
- Outcome
- was identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel stress-responsive rice NAC gene, ONAC022, was identified”
Claim · model-written summary
Drought, high salinity and ABA induced ONAC022 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- drought, high salinity, and abscisic acid (ABA)e0
- Gene
- ONAC022e0
- Outcome
- Expression of ONAC022e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of ONAC022 was induced by drought, high salinity, and abscisic acid (ABA).”
t0“Rice”
Claim · model-written summary
ONAC022 bound a canonical NAC recognition cis-element and showed C-terminal transactivation in yeast.
- Taxon
- RiceOryza sativat0
- Gene
- ONAC022e0
- Outcome
- bind specifically to a canonical NAC recognition cis-element sequence and showed transactivation activitye0
- Setting
- yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ONAC022 protein was found to bind specifically to a canonical NAC recognition cis-element sequence and showed transactivation activity at its C-terminus in yeast.”
t0“Rice”
Claim · model-written summary
ONAC022 localized to the nucleus during transient expression in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- ONAC022e0
- Outcome
- localized to nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ONAC022 protein was localized to nucleus when transiently expressed in Nicotiana benthamiana.”
Claim · model-written summary
ONAC022 overexpression increased rice drought tolerance, survival and growth relative to wild type under greenhouse drought.
- Taxon
- riceOryza sativae0
- Material
- N22oee0
- Treatment
- drought stresse0
- Gene
- ONAC022e0
- Outcome
- drought tolerance, leading to higher survival ratios and better growthe0
- Direction
- increasede0
- Comparator
- wild-type (WT) plantse0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress condition in greenhouse, soil-grown ONAC022-overexpressing (N22oe) transgenic rice plants showed an increased drought tolerance, leading to higher survival ratios and better growth than wild-type (WT) plants.”
Claim · model-written summary
N22oe rice had enhanced salt tolerance relative to WT in 150 mM NaCl.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Treatment
- 150 mM NaCle0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When grown hydroponically in Hogland solution supplemented with 150 mM NaCl, the N22oe plants displayed an enhanced salt tolerance and accumulated less Na(+) in roots and shoots as compared to WT plants.”
t0“Rice”
Claim · model-written summary
N22oe plants accumulated less Na(+) in roots and shoots than WT under salt treatment.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Organ / stage
- roots and shootse0
- Treatment
- 150 mM NaCle0
- Outcome
- Na(+) in roots and shootse0
- Direction
- lesse0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When grown hydroponically in Hogland solution supplemented with 150 mM NaCl, the N22oe plants displayed an enhanced salt tolerance and accumulated less Na(+) in roots and shoots as compared to WT plants.”
t0“Rice”
Claim · model-written summary
Under drought, N22oe plants had lower water loss and transpiration rates.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Treatment
- drought stresse0
- Outcome
- rates of water loss and transpiratione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress condition, the N22oe plants exhibited decreased rates of water loss and transpiration, reduced percentage of open stomata and increased contents of proline and soluble sugars.”
t0“Rice”
Claim · model-written summary
Under drought, N22oe plants had fewer open stomata.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Treatment
- drought stresse0
- Outcome
- percentage of open stomatae0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress condition, the N22oe plants exhibited decreased rates of water loss and transpiration, reduced percentage of open stomata and increased contents of proline and soluble sugars.”
t0“Rice”
Claim · model-written summary
Under drought, N22oe plants had more proline and soluble sugars.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Treatment
- drought stresse0
- Outcome
- contents of proline and soluble sugarse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress condition, the N22oe plants exhibited decreased rates of water loss and transpiration, reduced percentage of open stomata and increased contents of proline and soluble sugars.”
t0“Rice”
Claim · model-written summary
N22oe lines were more sensitive to exogenous ABA at germination and seedling growth.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Organ / stage
- seed germination and seedling growth stagese0
- Treatment
- exogenous ABAe0
- Outcome
- sensitivity to exogenous ABAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the N22oe lines showed increased sensitivity to exogenous ABA at seed germination and seedling growth stages but contained higher level of endogenous ABA.”
t0“Rice”
Claim · model-written summary
N22oe lines contained more endogenous ABA.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Outcome
- endogenous ABAe0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the N22oe lines showed increased sensitivity to exogenous ABA at seed germination and seedling growth stages but contained higher level of endogenous ABA.”
t0“Rice”
Claim · model-written summary
ABA biosynthetic, signaling, regulatory and late stress-responsive genes were upregulated in N22oe plants.
- Taxon
- RiceOryza sativat0
- Material
- N22oee0
- Outcome
- Expression of some ABA biosynthetic genese0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of some ABA biosynthetic genes (OsNCEDs and OsPSY), signaling and regulatory genes (OsPP2C02, OsPP2C49, OsPP2C68, OsbZIP23, OsAP37, OsDREB2a, and OsMYB2), and late stress-responsive genes (OsRAB21, OsLEA3, and OsP5CS1) was upregulated in N22oe plants.”
t0“Rice”
Claim · model-written summary
ONAC022 was interpreted to promote drought and salt tolerance through an ABA-mediated pathway.
- Taxon
- RiceOryza sativat0
- Gene
- ONAC022e0
- Outcome
- drought and salt stress tolerancee0
- Qualifier
- through modulating an ABA-mediated pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ONAC022 functions as a stress-responsive NAC with transcriptional activator activity and plays a positive role in drought and salt stress tolerance through modulating an ABA-mediated pathway.”
t0“Rice”
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