Abstract
BACKGROUND: The response regulators represent the elements of bacterial two-component system and have been characterized from dicot plants like Arabidopsis but little information is available on the monocots, including the cereal crops. The aim of this study was to characterize type-A response regulator genes from rice, and to investigate their expression in various organs as well as in response to different hormones, including cytokinin, and environmental stimuli. RESULTS: By analysis of the whole genome sequence of rice, we have identified ten genes encoding type-A response regulators based upon their high sequence identity within the receiver domain. The exon-intron organization, intron-phasing as well as chromosomal location of all the RT-PCR amplified rice (Oryza sativa) response regulator (OsRR) genes have been analyzed. The transcripts of OsRR genes could be detected by real-time PCR in all organs of the light- and dark-grown rice seedlings/plants, although there were quantitative differences. The steady-state transcript levels of most of the OsRR genes increased rapidly (within 15 min) on exogenous cytokinin application even in the presence of cycloheximide. Moreover, the expression of the OsRR6 gene was enhanced in rice seedlings exposed to salinity, dehydration and low temperature stress. CONCLUSION: Ten type-A response regulator genes identified in rice, the model monocot plant, show overlapping/differential expression patterns in various organs and in response to light. The induction of OsRR genes by cytokinin even in the absence of de novo protein synthesis qualifies them to be primary cytokinin response genes. The induction of OsRR6 in response to different environmental stimuli indicates its role in cross-talk between abiotic stress and cytokinin signaling. These results provide a foundation for further investigations on specific as well as overlapping cellular functions of type-A response regulators in rice.
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Claims 6
Claim · model-written summary
Ten type-A response regulator genes were identified in rice.
- Taxon
- rice
Oryza sativat0 - Outcome
- genes encoding type-A response regulatorse0
- Value
- ten genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we have identified ten genes encoding type-A response regulators”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsRR transcripts were detectable across organs of light- and dark-grown rice, with quantitative differences.
- Taxon
- riceOryza sativae0
- Organ / stage
- all organse0
- Gene
- OsRR genese0
- Outcome
- transcripts of OsRR genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcripts of OsRR genes could be detected by real-time PCR in all organs of the light- and dark-grown rice seedlings/plants, although there were quantitative differences.”
Claim · model-written summary
Most OsRR transcript levels increased rapidly after cytokinin application even with cycloheximide.
- Taxon
- riceOryza sativat0
- Treatment
- exogenous cytokinin applicatione0
- Gene
- OsRR genese0
- Outcome
- steady-state transcript levelse0
- Direction
- increasede0
- Value
- within 15 mine0
- Qualifier
- presence of cycloheximidee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The steady-state transcript levels of most of the OsRR genes increased rapidly (within 15 min) on exogenous cytokinin application even in the presence of cycloheximide.”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsRR6 expression rose under salinity stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the OsRR6 gene was enhanced in rice seedlings exposed to salinity, dehydration and low temperature stress”
Claim · model-written summary
OsRR6 expression rose under dehydration stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the OsRR6 gene was enhanced in rice seedlings exposed to salinity, dehydration and low temperature stress”
Claim · model-written summary
OsRR6 expression rose under low temperature stress.
- Taxon
- riceOryza sativae0
- Treatment
- low temperature stresse0
- Gene
- OsRR6 genee0
- Outcome
- expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the OsRR6 gene was enhanced in rice seedlings exposed to salinity, dehydration and low temperature stress”
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