Paper · 2019 · International Journal of Current Microbiology and Applied Sciences

Biological Control of Meloidogyne incognita by Trichoderma harzianum

Kurulkar Uday; Bhabesh Bhagawati; Pranjal Pratim Neog

10
claims
21
verbatim quotes
9
citations
2
research areas
✓
Open access · cc-by-nc-sa

Abstract

Trichoderma harzianum parasitism on Meloidogyne incognita eggs and juveniles was examined in-vitro under Assam condition. M. incognita egg masses, their derived eggs and second-stage juveniles (J2) were parasitized by T. harzianum. The conidia of the T. harzianum were found inside of the eggs and attached to the J2s with the gelatinous matrix. The eggs were penetrated and parasitized by the hyphae of T. harzianum, while eggs containing juveniles were also parasitised by T. harzianum. Further, isolate T. harzainum was used for to know the bio-efficacy against M. incognita infected on okra under pot condition. For this T. harzianum was applied either as a seed treatment and/or soil application or both. Carbosulfan as a seed treatment and carbofuran as soil application was applied as chemical checks both either singly or in combination. The results showed that either T. harzianum or the chemicals (Carbofuran and Carbosulfan) when applied together as a seed treatment and soil application, improved plant growth parameters of okra and reduced the nematode multiplication as compared to when they were applied either as a seed treatment or soil application. Application of chemicals either as a seed treatment or soil application emerged as the most effective treatment as compared to the T. harzianum. However, in respect of T. harzianum when applied together as a seed treatment and soil application showed significantly better results in an improving the plant growth parameters and reduction in the nematode multiplication as compared to the treatments with carbosulfan as a seed treatment and carbofuran as soil application alone.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-sa. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma harzianum parasitized Meloidogyne incognita eggs and juveniles in vitro.

Interactor
M. incognitaMeloidogyne incognitae0pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
eggs and second-stage juveniles (J2) were parasitizede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“M. incognita egg masses, their derived eggs and second-stage juveniles (J2) were parasitized by T. harzianum.”

✓ located verbatim in the source · abstract, characters 122–231 · show in text ↑
e1

“infected on okra”

✓ located verbatim in the source · abstract, characters 578–594 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma conidia occurred inside nematode eggs and attached to juveniles.

Interactor
M. incognitaMeloidogyne incognitae1pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
conidia of the T. harzianum were found inside of the eggs and attached to the J2se0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The conidia of the T. harzianum were found inside of the eggs and attached to the J2s with the gelatinous matrix.”

✓ located verbatim in the source · abstract, characters 232–345 · show in text ↑
e1

“M. incognita egg masses”

✓ located verbatim in the source · abstract, characters 122–145 · show in text ↑
e2

“infected on okra”

✓ located verbatim in the source · abstract, characters 578–594 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Trichoderma hyphae penetrated and parasitized nematode eggs, including eggs containing juveniles.

Interactor
M. incognitaMeloidogyne incognitae1pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
eggs were penetrated and parasitizede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The eggs were penetrated and parasitized by the hyphae of T. harzianum, while eggs containing juveniles were also parasitised by T. harzianum.”

✓ located verbatim in the source · abstract, characters 346–488 · show in text ↑
e1

“M. incognita egg masses”

✓ located verbatim in the source · abstract, characters 122–145 · show in text ↑
e2

“infected on okra”

✓ located verbatim in the source · abstract, characters 578–594 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined Trichoderma seed and soil application improved okra growth over a single application route.

Treatment
T. harzianume0
Interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
plant growth parameters of okrae0
Direction
improvede0
Comparator
either as a seed treatment or soil applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · InteractorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“either T. harzianum or the chemicals (Carbofuran and Carbosulfan) when applied together as a seed treatment and soil application, improved plant growth parameters of okra and reduced the nematode multiplication as compared to when they were applied either as a seed treatment or soil application.”

✓ located verbatim in the source · abstract, characters 870–1166 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined Trichoderma seed and soil application reduced nematode multiplication over a single application route.

Treatment
T. harzianume0
Interactor
M. incognitaMeloidogyne incognitae1pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
nematode multiplicatione0
Direction
reducede0
Comparator
either as a seed treatment or soil applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“either T. harzianum or the chemicals (Carbofuran and Carbosulfan) when applied together as a seed treatment and soil application, improved plant growth parameters of okra and reduced the nematode multiplication as compared to when they were applied either as a seed treatment or soil application.”

✓ located verbatim in the source · abstract, characters 870–1166 · show in text ↑
e1

“M. incognita egg masses”

✓ located verbatim in the source · abstract, characters 122–145 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined Carbofuran and Carbosulfan seed and soil application improved okra growth over a single application route.

Treatment
Carbofuran and Carbosulfane0
Outcome
plant growth parameters of okrae0
Direction
improvede0
Comparator
either as a seed treatment or soil applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the chemicals (Carbofuran and Carbosulfan) when applied together as a seed treatment and soil application, improved plant growth parameters of okra and reduced the nematode multiplication as compared to when they were applied either as a seed treatment or soil application.”

✓ located verbatim in the source · abstract, characters 893–1166 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combined chemical seed and soil application reduced nematode multiplication over a single application route.

Treatment
Carbofuran and Carbosulfane0
Interactor
M. incognitaMeloidogyne incognitae1pathogen
Outcome
nematode multiplicatione0
Direction
reducede0
Comparator
either as a seed treatment or soil applicatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the chemicals (Carbofuran and Carbosulfan) when applied together as a seed treatment and soil application, improved plant growth parameters of okra and reduced the nematode multiplication as compared to when they were applied either as a seed treatment or soil application.”

✓ located verbatim in the source · abstract, characters 893–1166 · show in text ↑
e1

“M. incognita egg masses”

✓ located verbatim in the source · abstract, characters 122–145 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chemical seed or soil treatment was more effective than Trichoderma treatment.

Treatment
chemicals either as a seed treatment or soil applicatione0
Outcome
most effective treatmente0
Direction
most effectivee0
Comparator
T. harzianume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Application of chemicals either as a seed treatment or soil application emerged as the most effective treatment as compared to the T. harzianum.”

✓ located verbatim in the source · abstract, characters 1167–1311 · show in text ↑
e1

“infected on okra”

✓ located verbatim in the source · abstract, characters 578–594 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined Trichoderma seed and soil application gave significantly better okra growth than individual chemical treatments.

Treatment
T. harzianum when applied together as a seed treatment and soil applicatione0
Outcome
plant growth parameterse0
Direction
improvinge0
Comparator
carbosulfan as a seed treatment and carbofuran as soil application alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum when applied together as a seed treatment and soil application showed significantly better results in an improving the plant growth parameters and reduction in the nematode multiplication as compared to the treatments with carbosulfan as a seed treatment and carbofuran as soil application alone.”

✓ located verbatim in the source · abstract, characters 1335–1644 · show in text ↑
e1

“infected on okra”

✓ located verbatim in the source · abstract, characters 578–594 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Combined Trichoderma seed and soil application gave significantly better nematode reduction than individual chemical treatments.

Treatment
T. harzianum when applied together as a seed treatment and soil applicatione0
Interactor
M. incognitaMeloidogyne incognitae2pathogen
Second interactor
T. harzianumTrichoderma harzianume0biocontrol_agent
Outcome
nematode multiplicatione0
Direction
reductione0
Comparator
carbosulfan as a seed treatment and carbofuran as soil application alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“T. harzianum when applied together as a seed treatment and soil application showed significantly better results in an improving the plant growth parameters and reduction in the nematode multiplication as compared to the treatments with carbosulfan as a seed treatment and carbofuran as soil application alone.”

✓ located verbatim in the source · abstract, characters 1335–1644 · show in text ↑
e1

“infected on okra”

✓ located verbatim in the source · abstract, characters 578–594 · show in text ↑
e2

“M. incognita egg masses”

✓ located verbatim in the source · abstract, characters 122–145 · show in text ↑

carbofuran · Meloidogyne incognita · Abelmoschus esculentus (okra)Positive and negative results

14 papers: 13 positive, 1 negative or no effect

12 papers in the same direction as this paper · 1 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

10 papers more in this group

See all 14 papers →

Trichoderma harzianum · Meloidogyne incognita · Abelmoschus esculentus (okra)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

carbosulfan · Meloidogyne incognita · Abelmoschus esculentus (okra)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

carbofuran · growth · Abelmoschus esculentus (okra)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Trichoderma harzianum · growth · Abelmoschus esculentus (okra)Results agree

9 papers: 9 positive, 0 negative or no effect

8 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

seed treatment · growth · Abelmoschus esculentus (okra)Positive and negative results

59 papers: 53 positive, 6 negative or no effect

52 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. negative
  3. negative

55 papers more in this group

See all 59 papers →

application · growth · Abelmoschus esculentus (okra)Positive and negative results

77 papers: 75 positive, 2 negative or no effect

74 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

73 papers more in this group

See all 77 papers →

application · Meloidogyne incognita · Abelmoschus esculentus (okra)Results agree

13 papers: 13 positive, 0 negative or no effect

12 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.