Paper · 2023 · International Journal of Pathogen Research

Effect of Soil Amendments on the Growth Parameters of Okra Infested with Root-Knot Nematode (Meloidogyne incognita)

Daniel Offiong Etim; Bassey Rosemary Aniete; Ituna Emini Eleng; et al.

14
claims
32
verbatim quotes
1
citations
3
research areas
✓
Open access

Abstract

Aims: To evaluate the efficacy of Trichoderma harzianum, Neem cake, and poultry manure on the growth performance of okra infected with Meloidogyne incognita. Study Design: The experiment was laid out in a completely randomized design. Place and Duration of Study: Department of Botany, University of Calabar, between April 2022 and August, 2022. Methodology: Soil samples were collected at the depth of 0-15cm using auger, heat sterilized and spread out in a shade for 7days to cool before use for planting okra seed. Galled roots of okra plants were blended and emptied into 1000ml beaker. This was the inoculum used for plant inoculation. Pure culture of T. harzianum inoculated into a steril petri dishes and allowed to sporulate. Neem cake and poultry manure were applied at the rate of 10g and 20g per planting pot. Holes were excavated around the base of the okra plant and 1g of T. harzianum spores was used for inoculation. Data were collected on plant height, number of leaves, petiole length, and percentage leaf abscission. Results: Number of leaves on pot treated with M. incognita, T. harzianum and 20 g of neem cake was significantly (p≤ 0.05) different from all other pots. Data obtained indicates that after 3 weeks of planting all the treatment combinations had significant (p≤0.05) impact on the crops grown in pots treated with 10 g of neem cake which was not significantly (p>0.05) different. Data obtained on plant height in pots with the treatment combinations (M1T1N0, M1T0N1, M1T0N2, M0T0N2, M0T0N0, M0T0P1 and M0T0P0) were significantly (p≤ 0.05) different. Results on petiole length grown in pots with treatment combinations (M1T1N2, M0T1N1 and M0T0P1) were not significantly (p> 0.05) different from the control. Results shows that the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%. Results from other treatments were as follows, M0T0N1 (62.3%), M1T1N0 (58.6%), M0T0N2 (56.3%), M1T0N1 (52.9%) and M1T1N2 (51.2%). Result obtained on soil amendment with poultry manure revealed that pots treated with 20t/ha of poultry manure had impact on plant height which was significant (p≤ 0.05). Conclusion: Based on the data obtained on the growth parameters, it can be concluded that T. harzianum and neem cake significantly increased the growth of okra infested with M. incognita and the impact was significantly shown on the plant height and petiole length. Number of leaves were significantly different at 3 and 5 weeks after planting. T. harzianum has be proven to be of economic value in agriculture both as biocontrol agent and soil nutrient booster.

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Claims 14

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Trichoderma harzianum and neem cake significantly increased the growth of okra infested with M. incognita.

Treatment
T. harzianum and neem cakee1
Interactor
M. incognitaMeloidogyne incognitae1pathogen
Second interactor
T. harzianumTrichoderma harzianume1biocontrol_agent
Outcome
growthe1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗
Trichoderma harzianum
Trichoderma harzianum · Second interactorUS Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e1

“T. harzianum and neem cake significantly increased the growth of okra infested with M. incognita”

✓ located verbatim in the source · abstract, characters 2266–2362 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Number of leaves was significantly different in okra pots treated with M. incognita, T. harzianum and 20 g of neem cake from all other pots.

Treatment
20 g of neem cakee2
Interactor
M. incognitaMeloidogyne incognitae2pathogen
Outcome
Number of leavese2
Direction
differente2
Comparator
all other potse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Meloidogyne incognita
Meloidogyne incognita · InteractorPhoto by William Wergin and Richard Sayre. Colorized by Stephen Ausmus. U.S. Department of Agriculture · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e2

“Number of leaves on pot treated with M. incognita, T. harzianum and 20 g of neem cake was significantly (p≤ 0.05) different from all other pots”

✓ located verbatim in the source · abstract, characters 1044–1187 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

After three weeks, all treatment combinations had a significant impact on the crops in the pots.

Treatment
all the treatment combinationse3
Outcome
impact on the cropse3

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e3

“Data obtained indicates that after 3 weeks of planting all the treatment combinations had significant (p≤0.05) impact on the crops grown in pots treated with 10 g of neem cake which was not significantly (p>0.05) different”

✓ located verbatim in the source · abstract, characters 1189–1411 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Pots treated with 10 g of neem cake were not significantly different in crop growth.

Treatment
10 g of neem cakee3
Outcome
crops grown in potse3
Direction
differente3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e3

“Data obtained indicates that after 3 weeks of planting all the treatment combinations had significant (p≤0.05) impact on the crops grown in pots treated with 10 g of neem cake which was not significantly (p>0.05) different”

✓ located verbatim in the source · abstract, characters 1189–1411 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Plant height differed significantly among the okra pots with the listed treatment combinations.

Treatment
treatment combinationse4
Outcome
plant heighte4
Direction
differente4
Qualifier
M1T1N0, M1T0N1, M1T0N2, M0T0N2, M0T0N0, M0T0P1 and M0T0P0e4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e4

“Data obtained on plant height in pots with the treatment combinations (M1T1N0, M1T0N1, M1T0N2, M0T0N2, M0T0N0, M0T0P1 and M0T0P0) were significantly (p≤ 0.05) different”

✓ located verbatim in the source · abstract, characters 1413–1581 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Petiole length in the listed treatment combinations was not significantly different from the control.

Treatment
M1T1N2, M0T1N1 and M0T0P1e5
Outcome
petiole lengthe5
Direction
not significantlye5
Comparator
controle5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e5

“Results on petiole length grown in pots with treatment combinations (M1T1N2, M0T1N1 and M0T0P1) were not significantly (p> 0.05) different from the control”

✓ located verbatim in the source · abstract, characters 1583–1738 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The highest percentage of abscised leaves was observed on okra treated with T. harzianum and neem cake, at 66.4%.

Treatment
T. harzianum and Neem cakee6
Outcome
percentage of abscised leavese6
Direction
higheste6
Value
66.4%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e6

“the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%”

✓ located verbatim in the source · abstract, characters 1759–1873 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Okra treated with M0T0N1 had 62.3% abscised leaves.

Treatment
M0T0N1e7
Outcome
percentage of abscised leavese6
Value
62.3%e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e6

“the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%”

✓ located verbatim in the source · abstract, characters 1759–1873 · show in text ↑
e7

“M0T0N1 (62.3%)”

✓ located verbatim in the source · abstract, characters 1922–1936 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Okra treated with M1T1N0 had 58.6% abscised leaves.

Treatment
M1T1N0e7
Outcome
percentage of abscised leavese6
Value
58.6%e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e6

“the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%”

✓ located verbatim in the source · abstract, characters 1759–1873 · show in text ↑
e7

“M1T1N0 (58.6%)”

✓ located verbatim in the source · abstract, characters 1938–1952 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Okra treated with M0T0N2 had 56.3% abscised leaves.

Treatment
M0T0N2e7
Outcome
percentage of abscised leavese6
Value
56.3%e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e6

“the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%”

✓ located verbatim in the source · abstract, characters 1759–1873 · show in text ↑
e7

“M0T0N2 (56.3%)”

✓ located verbatim in the source · abstract, characters 1954–1968 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Okra treated with M1T0N1 had 52.9% abscised leaves.

Treatment
M1T0N1e7
Outcome
percentage of abscised leavese6
Value
52.9%e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e6

“the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%”

✓ located verbatim in the source · abstract, characters 1759–1873 · show in text ↑
e7

“M1T0N1 (52.9%)”

✓ located verbatim in the source · abstract, characters 1970–1984 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Okra treated with M1T1N2 had 51.2% abscised leaves.

Treatment
M1T1N2e7
Outcome
percentage of abscised leavese6
Value
51.2%e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e6

“the highest percentage of abscised leaves was observed on plant treated with T. harzianum and Neem cake with 66.4%”

✓ located verbatim in the source · abstract, characters 1759–1873 · show in text ↑
e7

“M1T1N2 (51.2%)”

✓ located verbatim in the source · abstract, characters 1989–2003 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Poultry manure at 20 t/ha had a significant impact on okra plant height.

Treatment
20t/ha of poultry manuree7
Outcome
plant heighte7

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e7

“pots treated with 20t/ha of poultry manure had impact on plant height which was significant (p≤ 0.05)”

✓ located verbatim in the source · abstract, characters 2073–2174 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Number of leaves of okra differed significantly at 3 and 5 weeks after planting.

Outcome
Number of leavese7
Qualifier
at 3 and 5 weeks after plantinge7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the growth performance of okra infected with Meloidogyne incognita”

✓ located verbatim in the source · abstract, characters 90–156 · show in text ↑
e7

“Number of leaves were significantly different at 3 and 5 weeks after planting”

✓ located verbatim in the source · abstract, characters 2442–2519 · show in text ↑

Trichoderma harzianum · Meloidogyne incognita · Abelmoschus esculentus (okra)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

botanical · Meloidogyne incognita · Abelmoschus esculentus (okra)Results agree

26 papers: 26 positive, 0 negative or no effect

25 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

22 papers more in this group

See all 26 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.