Abstract
Rapid and efficient propagation methods that avoid the use of mutagenic growth regulators are required for date palm breeding and cultivation. The effects of different growth regulators on micropropagation of six female cultivars of date palm including Barhee, Medjool, Khalas, Khadrawi, Nemeishi and River Gem and the male cultivar Jarvis were invstigated. The experiments were conducted in vitro using floral explants of female and male mature inflorescences. Embryogenic callus induction and proliferation were significantly higher (93%) in the woody plant nutrient culture medium (WPM) supplemented with 5mg l -1 Thidiazuron (TDZ). The addition of zeatin to the medium was most effective for shoot regeneration from callus and this enhanced regeneration frequency and the average number of shoots obtained per explant. The highest number of shoots/explant (16) was obtained using 2 mg l -1 zeatin in WPM and WPM with 0.5 mg l -1 GA 3 and 2 mg l -1 zeatin enhanced shoot length significantly. Bud initiation and adventitious shoot formation 16 weeks after flower culture was enhanced by WPM supplemented with 1.5 mg l -1 2,4-D and 5 mg l -1 2ip. Sub-culturing of formed buds on a multiplication medium supplemented with 3.0 mg l -1 zeatin and 0.3 mg l -1
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Claims 5
Claim · model-written summary
Thidiazuron (5 mg/l) in WPM significantly increased embryogenic callus induction and proliferation in date palm to 93%
- Taxon
- date palm
Phoenix dactyliferat0 - Treatment
- 5mg l -1 Thidiazuron (TDZ)e1
- Outcome
- Embryogenic callus induction and proliferatione1
- Direction
- highere1
- Value
- 93%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cultivars”
e1“Embryogenic callus induction and proliferation were significantly higher (93%) in the woody plant nutrient culture medium (WPM) supplemented with 5mg l -1 Thidiazuron (TDZ)”
Claim · model-written summary
Zeatin was the most effective addition for shoot regeneration from date palm callus, enhancing regeneration frequency and shoots per explant
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- addition of zeatine2
- Outcome
- shoot regeneration from calluse2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cultivars”
e2“The addition of zeatin to the medium was most effective for shoot regeneration from callus and this enhanced regeneration frequency and the average number of shoots obtained per explant”
Claim · model-written summary
Two mg/l zeatin in WPM gave the highest number of shoots per explant in date palm (16)
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- 2 mg l -1 zeatin in WPMe3
- Outcome
- number of shoots/explante3
- Direction
- higheste3
- Value
- 16e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cultivars”
e3“The highest number of shoots/explant (16) was obtained using 2 mg l -1 zeatin in WPM”
Claim · model-written summary
WPM with 0.5 mg/l GA3 and 2 mg/l zeatin significantly enhanced shoot length in date palm
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- 0.5 mg l -1 GA 3 and 2 mg l -1 zeatine4
- Outcome
- shoot lengthe4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cultivars”
e4“WPM with 0.5 mg l -1 GA 3 and 2 mg l -1 zeatin enhanced shoot length significantly”
Claim · model-written summary
WPM with 1.5 mg/l 2,4-D and 5 mg/l 2ip enhanced bud initiation and adventitious shoot formation 16 weeks after flower culture in date palm
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- 1.5 mg l -1 2,4-D and 5 mg l -1 2ipe5
- Outcome
- Bud initiation and adventitious shoot formatione5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cultivars”
e5“Bud initiation and adventitious shoot formation 16 weeks after flower culture was enhanced by WPM supplemented with 1.5 mg l -1 2,4-D and 5 mg l -1 2ip”
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