Abstract
The purpose of this study was to check the possibility of callus and somatic embryogenesis in date palm cv. Khazravi using different explants including leaves, green fruit, immature seed, endosperm and green fruit pericarp. In the culture of leaves in the medium contains different plant growth regulators including 2,4-D, BAP and TDZ, highest callus induction was achieved in medium supplemented with, 5 mg.L-1 2,4-D, 2 mg.L-1 BAP and 4 mg.L-1 TDZ, and in treatment with 5 mg.L-1 2,4-D, 1 mg.L-1 BAP and 4 mg.L-1 TDZ. By using explant of immature fruit, found that when two growths regulative, TDZ and 2, 4-D in amount of 5 mg.L-1 are in the culture medium callugenesis and embryogenesis was achieved. The endosperm cultivation with pericarp and embryo in the same medium showed that the presence of two growth regulators 2,4-D and TDZ have been useful for callus production. Also in endosperm with zygotic embryo culture, embryo development and the root and shoot were formed after three months. Endosperm culture, in the culture medium containing various auxins and pericarp culture showed no any reactions. In general, these results showed that in date palm cv. Khazravi, treating the explants of leave and immature fruits with appropriate concentrations of plant growth regulators auxin and cytokinin, can induce callus and embryo.
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Claims 7
Claim · model-written summary
Leaf explants had highest callus induction with 5 mg.L-1 2,4-D, 2 mg.L-1 BAP and 4 mg.L-1 TDZ.
- Taxon
- date palm cv. Khazravi
Phoenix dactyliferat0 - Organ / stage
- leavest0
- Treatment
- 5 mg.L-1 2,4-D, 2 mg.L-1 BAP and 4 mg.L-1 TDZe0
- Outcome
- callus inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest callus induction was achieved in medium supplemented with, 5 mg.L-1 2,4-D, 2 mg.L-1 BAP and 4 mg.L-1 TDZ, and in treatment with 5 mg.L-1 2,4-D, 1 mg.L-1 BAP and 4 mg.L-1 TDZ.”
t0“date palm cv. Khazravi using leaves, immature fruit and endosperm explants”
Claim · model-written summary
Leaf explants had highest callus induction with 5 mg.L-1 2,4-D, 1 mg.L-1 BAP and 4 mg.L-1 TDZ.
- Taxon
- date palm cv. KhazraviPhoenix dactyliferat0
- Organ / stage
- leavest0
- Treatment
- 5 mg.L-1 2,4-D, 1 mg.L-1 BAP and 4 mg.L-1 TDZe0
- Outcome
- callus inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest callus induction was achieved in medium supplemented with, 5 mg.L-1 2,4-D, 2 mg.L-1 BAP and 4 mg.L-1 TDZ, and in treatment with 5 mg.L-1 2,4-D, 1 mg.L-1 BAP and 4 mg.L-1 TDZ.”
t0“date palm cv. Khazravi using leaves, immature fruit and endosperm explants”
Claim · model-written summary
Immature fruit explants formed callus and embryos with TDZ and 2, 4-D.
- Taxon
- date palm cv. KhazraviPhoenix dactyliferat0
- Organ / stage
- immature fruitt0
- Treatment
- TDZ and 2, 4-D in amount of 5 mg.L-1e0
- Outcome
- callugenesis and embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when two growths regulative, TDZ and 2, 4-D in amount of 5 mg.L-1 are in the culture medium callugenesis and embryogenesis was achieved.”
t0“date palm cv. Khazravi using leaves, immature fruit and endosperm explants”
Claim · model-written summary
Endosperm cultivated with pericarp and embryo responded to 2,4-D and TDZ with callus production.
- Taxon
- date palm cv. KhazraviPhoenix dactyliferat0
- Organ / stage
- endosperme0
- Treatment
- 2,4-D and TDZe0
- Outcome
- callus productione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The endosperm cultivation with pericarp and embryo in the same medium showed that the presence of two growth regulators 2,4-D and TDZ have been useful for callus production.”
t0“date palm cv. Khazravi”
Claim · model-written summary
Endosperm with zygotic embryo culture formed an embryo, root and shoot after three months.
- Taxon
- date palm cv. KhazraviPhoenix dactyliferat0
- Organ / stage
- endosperm with zygotic embryoe0
- Outcome
- embryo development and the root and shoot were formede0
- Qualifier
- after three monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Also in endosperm with zygotic embryo culture, embryo development and the root and shoot were formed after three months.”
t0“date palm cv. Khazravi”
Claim · model-written summary
Endosperm culture with auxins showed no reaction.
- Taxon
- date palm cv. KhazraviPhoenix dactyliferat0
- Organ / stage
- Endosperme0
- Treatment
- various auxinse0
- Outcome
- no any reactionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endosperm culture, in the culture medium containing various auxins and pericarp culture showed no any reactions.”
t0“date palm cv. Khazravi”
Claim · model-written summary
Pericarp culture showed no reaction.
- Taxon
- date palm cv. KhazraviPhoenix dactyliferat0
- Organ / stage
- pericarpe0
- Outcome
- no any reactionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endosperm culture, in the culture medium containing various auxins and pericarp culture showed no any reactions.”
t0“date palm cv. Khazravi”
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