Abstract
Plant regeneration from immature female inflorescences of date palm (Phoenix dactylifera L.) cultivar Siwi (semi-dry cv.), via somatic embryogenesis was achieved. Immature inflorescences explants were cultured on Murashige and Skoog (MS) medium supplemented with TDZ at 1.0 mg/l. Endogenous hormones (GA3, IAA, Zeatin and ABA), total soluble sugars (reducing and non-reducing sugars), free amino acids, indoles and phenols were determined. The levels of GA3 and IAA in explant reached the highest values and then decreased in callus stage. Zeatin, IAA, and ABA levels were higher in embryogenic callus. GA3 and zeatin in mature somatic embryo maintained a relatively high level while IAA and ABA dropped. The highest significant values of total soluble sugars and their fractions were noted in explant stage. Embryogenic callus stage induced a significant increase in total soluble sugars, free amino acids, phenols and indoles concentrations as compared to callus and mature somatic embryo. Callus induction was achieved by TDZ after 4–5 weeks of culture. The callus differentiated into embryogenic calli on free cytokinin medium. Light microscope observations revealed that the callus consisted of two different types of cells, soft vacuolated cells which did not implicate in embryo formation and compact aggregate cell masses which have the ability to generate the somatic embryos.
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Claims 10
Claim · model-written summary
Date palm plants were regenerated from immature female inflorescences via somatic embryogenesis.
- Taxon
- date palm
Phoenix dactyliferae0 - Material
- Siwie0
- Organ / stage
- immature female inflorescencese0
- Outcome
- Plant regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant regeneration from immature female inflorescences of date palm (Phoenix dactylifera L.) cultivar Siwi (semi-dry cv.), via somatic embryogenesis was achieved”
Claim · model-written summary
GA3 and IAA reached highest values in explants and then decreased at the callus stage.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- explante0
- Outcome
- levels of GA3 and IAAe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The levels of GA3 and IAA in explant reached the highest values and then decreased in callus stage”
t0“date palm”
Claim · model-written summary
Zeatin, IAA, and ABA levels were higher in embryogenic callus.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- embryogenic calluse0
- Outcome
- Zeatin, IAA, and ABA levelse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zeatin, IAA, and ABA levels were higher in embryogenic callus”
t0“date palm”
Claim · model-written summary
GA3 and zeatin remained relatively high in mature somatic embryos.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- mature somatic embryoe0
- Outcome
- GA3 and zeatine0
- Direction
- relatively highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 and zeatin in mature somatic embryo maintained a relatively high level”
t0“date palm”
Claim · model-written summary
IAA and ABA dropped in mature somatic embryos.
- Taxon
- date palmPhoenix dactyliferat0
- Outcome
- IAA and ABAe0
- Direction
- droppede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while IAA and ABA dropped”
t0“date palm”
Claim · model-written summary
Total soluble sugars and their fractions had highest significant values at explant stage.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- explant stagee0
- Outcome
- total soluble sugars and their fractionse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest significant values of total soluble sugars and their fractions were noted in explant stage”
t0“date palm”
Claim · model-written summary
Embryogenic callus had significantly increased total soluble sugars, free amino acids, phenols and indoles compared with callus and mature embryos.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- Embryogenic callus stagee0
- Outcome
- total soluble sugars, free amino acids, phenols and indoles concentrationse0
- Direction
- increasee0
- Comparator
- callus and mature somatic embryoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic callus stage induced a significant increase in total soluble sugars, free amino acids, phenols and indoles concentrations as compared to callus and mature somatic embryo”
t0“date palm”
Claim · model-written summary
TDZ induced callus after 4–5 weeks of culture.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- TDZe0
- Outcome
- Callus inductione0
- Value
- 4–5 weekse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction was achieved by TDZ after 4–5 weeks of culture”
t0“date palm”
Claim · model-written summary
Callus differentiated into embryogenic calli on cytokinin-free medium.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- calluse0
- Treatment
- free cytokinin mediume0
- Outcome
- differentiated into embryogenic callie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The callus differentiated into embryogenic calli on free cytokinin medium”
t0“date palm”
Claim · model-written summary
Callus contained soft vacuolated cells that did not form embryos and compact aggregate cells capable of generating somatic embryos.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- calluse0
- Outcome
- two different types of cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the callus consisted of two different types of cells, soft vacuolated cells which did not implicate in embryo formation and compact aggregate cell masses which have the ability to generate the somatic embryos”
t0“date palm”
The same questions in other papers
Thidiazuron · growth · Phoenix dactylifera (date palm)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.