Abstract
Despite the increasing understanding of the stress-responsive roles of zinc-finger RNA-binding proteins (RZs) in several plant species, such as Arabidopsis thaliana, wheat (Triticum aestivum) and rice (Oryza sativa), the functions of RZs in cabbage (Brassica rapa) have not yet been elucidated. In this study, the functional roles of the three RZ family members present in the cabbage genome, designated as BrRZ1, BrRZ2 and BrRZ3, were investigated in transgenic Arabidopsis under normal and environmental stress conditions. Subcellular localization analysis revealed that all BrRZ proteins were exclusively localized in the nucleus. The expression levels of each BrRZ were markedly increased by cold, drought or salt stress and by abscisic acid (ABA) treatment. Expression of BrRZ3 in Arabidopsis retarded seed germination and stem growth and reduced seed yield of Arabidopsis plants under normal growth conditions. Germination of BrRZ2- or BrRZ3-expressing Arabidopsis seeds was delayed compared with that of wild-type seeds under dehydration or salt stress conditions and cold stress conditions, respectively. Seedling growth of BrRZ3-expressing transgenic Arabidopsis plants was significantly inhibited under salt, dehydration or cold stress conditions. Notably, seedling growth of all three BrRZ-expressing transgenic Arabidopsis plants was inhibited upon ABA treatment. Importantly, all BrRZs possessed RNA chaperone activity. Taken together, these results indicate that the three cabbage BrRZs harboring RNA chaperone activity play diverse roles in seed germination and seedling growth of plants under abiotic stress conditions as well as in the presence of ABA.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
All cabbage BrRZ proteins were exclusively localized in the nucleus.
- Taxon
- cabbage
Brassica oleracea var. capitatat0 - Gene
- BrRZ proteinse2
- Outcome
- Subcellular localizatione2
- Qualifier
- exclusively localized in the nucleuse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e2“Subcellular localization analysis revealed that all BrRZ proteins were exclusively localized in the nucleus”
Claim · model-written summary
Expression levels of each cabbage BrRZ were markedly increased by cold, drought or salt stress.
- Taxon
- cabbageBrassica oleracea var. capitatat0
- Treatment
- cold, drought or salt stresse3
- Gene
- BrRZe3
- Outcome
- expression levels of each BrRZe3
- Direction
- markedly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e3“The expression levels of each BrRZ were markedly increased by cold, drought or salt stress and by abscisic acid (ABA) treatment”
Claim · model-written summary
Expression levels of each cabbage BrRZ were markedly increased by abscisic acid treatment.
- Taxon
- cabbageBrassica oleracea var. capitatat0
- Treatment
- abscisic acid (ABA) treatmente3
- Gene
- BrRZe3
- Outcome
- expression levels of each BrRZe3
- Direction
- markedly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e3“The expression levels of each BrRZ were markedly increased by cold, drought or salt stress and by abscisic acid (ABA) treatment”
Claim · model-written summary
BrRZ3 expression in Arabidopsis retarded seed germination and stem growth under normal growth conditions.
- Taxon
- Arabidopsis
Arabidopsise4 - Treatment
- Expression of BrRZ3e4
- Gene
- BrRZ3e4
- Outcome
- seed germination and stem growthe4
- Direction
- retardede4
- Qualifier
- under normal growth conditionse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e4“Expression of BrRZ3 in Arabidopsis retarded seed germination and stem growth and reduced seed yield of Arabidopsis plants under normal growth conditions”
Claim · model-written summary
BrRZ3 expression in Arabidopsis reduced seed yield under normal growth conditions.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- Expression of BrRZ3e4
- Gene
- BrRZ3e4
- Outcome
- seed yielde4
- Direction
- reducede4
- Qualifier
- under normal growth conditionse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e4“Expression of BrRZ3 in Arabidopsis retarded seed germination and stem growth and reduced seed yield of Arabidopsis plants under normal growth conditions”
Claim · model-written summary
BrRZ3-expressing Arabidopsis seedling growth was significantly inhibited under salt, dehydration or cold stress.
- Taxon
- ArabidopsisArabidopsise5
- Treatment
- salt, dehydration or cold stress conditionse5
- Gene
- BrRZ3e5
- Outcome
- Seedling growthe5
- Direction
- inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e5“Seedling growth of BrRZ3-expressing transgenic Arabidopsis plants was significantly inhibited under salt, dehydration or cold stress conditions”
Claim · model-written summary
BrRZ2-expressing Arabidopsis seeds germinated later than wild-type seeds under dehydration or salt stress.
- Taxon
- ArabidopsisArabidopsise6
- Treatment
- dehydration or salt stress conditionse6
- Gene
- BrRZ2e6
- Outcome
- Germinatione6
- Direction
- delayede6
- Comparator
- wild-type seedse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e6“Germination of BrRZ2- or BrRZ3-expressing Arabidopsis seeds was delayed compared with that of wild-type seeds under dehydration or salt stress conditions”
Claim · model-written summary
BrRZ3-expressing Arabidopsis seeds germinated later than wild-type seeds under cold stress.
- Taxon
- ArabidopsisArabidopsise6
- Treatment
- cold stress conditionse7
- Gene
- BrRZ3e6
- Outcome
- Germinatione6
- Direction
- delayede6
- Comparator
- wild-type seedse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e6“Germination of BrRZ2- or BrRZ3-expressing Arabidopsis seeds was delayed compared with that of wild-type seeds under dehydration or salt stress conditions”
e7“and cold stress conditions, respectively”
Claim · model-written summary
Seedling growth of all three BrRZ-expressing Arabidopsis plants was inhibited by ABA treatment.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- ABA treatmente1
- Gene
- BrRZe1
- Outcome
- seedling growthe1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e1“seedling growth of all three BrRZ-expressing transgenic Arabidopsis plants was inhibited upon ABA treatment”
Claim · model-written summary
All cabbage BrRZ proteins possessed RNA chaperone activity.
- Taxon
- cabbageBrassica oleracea var. capitatat0
- Gene
- BrRZse1
- Outcome
- RNA chaperone activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cabbage (Brassica rapa) play diverse roles”
e1“all BrRZs possessed RNA chaperone activity”
The same questions in other papers
(+)-abscisic acid · growth · ArabidopsisPositive and negative results
12 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
22 papers more in this group
See all 26 papers →salinity · growth · ArabidopsisResults differ
21 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
21 papers more in this group
See all 25 papers →cold · growth · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
8 papers more in this group
See all 12 papers →drought · growth · ArabidopsisPositive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
8 papers more in this group
See all 12 papers →transgenic · yield · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →transgenic · growth · ArabidopsisPositive and negative results
49 papers in the same direction as this paper · 35 in the opposite direction
- positive
- positive
- positive
81 papers more in this group
See all 85 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.