Abstract
It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair in Arabidposis. However, important questions remain regarding the biogenesis and function of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to characterize diRNAs in Arabidopsis and rice. We found that 21-nt diRNAs were generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9. Unexpectedly, Pol II transcription of the transgene was required for efficient diRNA production and the level of diRNA accumulation correlated with the expression level of the transgene. diRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes in Arabidopsis or rice. We also found that DCL4 and RDR6 that are known to be involved in posttranscriptional gene silencing were required to generate diRNAs. Our results suggest that DSBs are necessary but not sufficient for efficient diRNA generation and a high level of diRNAs is not necessary for DSB repair.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
A 21-nt diRNA was generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9 in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- 35S promoter::GU-US reporter transgenee0
- Treatment
- targeted by CRISPR/Cas9e0
- Outcome
- 21-nt diRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found that 21-nt diRNAs were generated from a 35S promoter::GU-US reporter transgene targeted by CRISPR/Cas9”
e1“to characterize diRNAs in Arabidopsis and rice”
Claim · model-written summary
Pol II transcription of the transgene was required for efficient diRNA production in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Pol II transcription of the transgenee2
- Outcome
- efficient diRNA productione2
- Direction
- requirede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“to characterize diRNAs in Arabidopsis and rice”
e2“Unexpectedly, Pol II transcription of the transgene was required for efficient diRNA production”
Claim · model-written summary
diRNA accumulation increased with the expression level of the transgene in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Material
- transgenee3
- Outcome
- diRNA accumulatione3
- Direction
- increaseinferrede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“to characterize diRNAs in Arabidopsis and rice”
e3“the level of diRNA accumulation correlated with the expression level of the transgene”
Claim · model-written summary
diRNAs were not detected from CRISPR/Cas9- or TALEN-induced double-strand breaks within the examined endogenous genes in Arabidopsis or rice.
- Taxon
- Arabidopsis or riceArabidopsis
Oryza sativae4 - Treatment
- CRISPR/Cas9- or TALEN-induced DSBse4
- Outcome
- diRNAse4
- Qualifier
- within the examined endogenous genese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“diRNAs were not detected from CRISPR/Cas9- or TALEN-induced DSBs within the examined endogenous genes in Arabidopsis or rice”
Claim · model-written summary
DCL4 and RDR6 were required to generate diRNAs in Arabidopsis and rice.
- Taxon
- Arabidopsis and riceArabidopsisOryza sativae1
- Gene
- DCL4 and RDR6e5
- Outcome
- generate diRNAse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“to characterize diRNAs in Arabidopsis and rice”
e5“We also found that DCL4 and RDR6 that are known to be involved in posttranscriptional gene silencing were required to generate diRNAs”
Claim · model-written summary
Double-strand breaks are necessary but not sufficient for efficient diRNA generation in Arabidopsis and rice.
- Taxon
- Arabidopsis and riceArabidopsisOryza sativae1
- Outcome
- efficient diRNA generatione6
- Qualifier
- DSBs are necessary but not sufficiente6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“to characterize diRNAs in Arabidopsis and rice”
e6“DSBs are necessary but not sufficient for efficient diRNA generation”
Claim · model-written summary
A high level of diRNAs is not necessary for double-strand break repair in Arabidopsis and rice.
- Taxon
- Arabidopsis and riceArabidopsisOryza sativae1
- Outcome
- DSB repaire7
- Qualifier
- a high level of diRNAs is not necessarye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“to characterize diRNAs in Arabidopsis and rice”
e7“a high level of diRNAs is not necessary for DSB repair”
The same questions in other papers
transgenic · yield · ArabidopsisPositive and negative results
10 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.