Abstract
Five coarse cultivars of rice (Oryza sativa L.) viz., Pakhal, Swat-1, JP-5, KS-282 and IR-6 were evaluated for their ability to undergo somatic embryogenesis. Results indicated that N6 basal medium supplemented with 2 mg/L of 2, 4-D without BAP was the most appropriate for callus growth but inferior in its regeneration potential. Among all the genotype tested JP-5 showed highest 84 %) whereas IR-6 showed the lowest (63 %) callus induction frequency. (Regeneration rate was highest (82 %) on N6 basal media supplemented with 5 mg/L of BAP and 0.5 mg/L of IAA. All of the varieties showed a good regeneration potential on culturing, the highest for KS-282 and Pakhal while JP-5 showing the lowest regeneration compared with other genotypes studies. It was observed that sucrose supported regeneration better than sorbitol and mannitol. Calli on sucrose containing media showed higher regeneration and produced longer shoots whereas mannitol could not support the growth.
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Claims 10
Claim · model-written summary
N6 with 2 mg/L 2, 4-D without BAP was most appropriate for callus growth.
- Taxon
- rice (Oryza sativa L.)
Oryza sativat0 - Treatment
- N6 basal medium supplemented with 2 mg/L of 2, 4-D without BAPe0
- Outcome
- callus growthe0
- Direction
- most appropriatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results indicated that N6 basal medium supplemented with 2 mg/L of 2, 4-D without BAP was the most appropriate for callus growth but inferior in its regeneration potential.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
This medium had inferior regeneration potential.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- N6 basal medium supplemented with 2 mg/L of 2, 4-D without BAPe0
- Outcome
- regeneration potentiale0
- Direction
- inferiore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results indicated that N6 basal medium supplemented with 2 mg/L of 2, 4-D without BAP was the most appropriate for callus growth but inferior in its regeneration potential.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
JP-5 had the highest callus induction frequency, 84 %.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Material
- JP-5e0
- Outcome
- callus induction frequencye0
- Direction
- higheste0
- Value
- 84 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among all the genotype tested JP-5 showed highest 84 %) whereas IR-6 showed the lowest (63 %) callus induction frequency.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
IR-6 had the lowest callus induction frequency, 63 %.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Material
- IR-6e0
- Outcome
- callus induction frequencye0
- Direction
- loweste0
- Value
- 63 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among all the genotype tested JP-5 showed highest 84 %) whereas IR-6 showed the lowest (63 %) callus induction frequency.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Regeneration rate was highest, 82 %, on N6 with BAP and IAA.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- N6 basal media supplemented with 5 mg/L of BAP and 0.5 mg/L of IAAe0
- Outcome
- Regeneration ratee0
- Direction
- higheste0
- Value
- 82 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regeneration rate was highest (82 %) on N6 basal media supplemented with 5 mg/L of BAP and 0.5 mg/L of IAA.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
KS-282 and Pakhal had the highest regeneration.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Material
- KS-282 and Pakhale0
- Outcome
- regeneratione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All of the varieties showed a good regeneration potential on culturing, the highest for KS-282 and Pakhal while JP-5 showing the lowest regeneration compared with other genotypes studies.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
JP-5 had the lowest regeneration.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Material
- JP-5e0
- Outcome
- regeneratione0
- Direction
- loweste0
- Comparator
- other genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All of the varieties showed a good regeneration potential on culturing, the highest for KS-282 and Pakhal while JP-5 showing the lowest regeneration compared with other genotypes studies.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Sucrose supported regeneration better than sorbitol and mannitol.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- sucrosee0
- Outcome
- regeneratione0
- Direction
- bettere0
- Comparator
- sorbitol and mannitole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was observed that sucrose supported regeneration better than sorbitol and mannitol.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Sucrose media produced longer shoots.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- sucrose containing mediae0
- Outcome
- shootse0
- Direction
- longere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calli on sucrose containing media showed higher regeneration and produced longer shoots whereas mannitol could not support the growth.”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Mannitol did not support growth.
- Taxon
- rice (Oryza sativa L.)Oryza sativat0
- Treatment
- mannitole0
- Outcome
- growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calli on sucrose containing media showed higher regeneration and produced longer shoots whereas mannitol could not support the growth.”
t0“rice (Oryza sativa L.)”
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