Abstract
Aromatic rice MRQ 74, MRQ 80 and MRQ 50 are the most popular rice in Malaysia. Establishing an efficient regeneration protocols via somatic embryogenesis is required for varietal improvements. Somatic embryogenesis system was established in three aromatic rice varieties by using mature seeds. Optimised MS-B5 medium containing 1 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 10 mg/L NAA (α-naphthaleneacetic acid) produced highest production of embryogenic callus (89%) without any browning effect. The highest whitish somatic embryos frequency (75 %) was initiated by incubating embryogenic calli on same media containing 10 mg/L ABA and 9 g/L Gelrite agar for 8 weeks. However, the numbers of regenerated plantlets on medium containing NAA and 2,4-D which was previously pre-treated with 10 mg/L ABA, 9 g/L Gelrite agar and incubation at 8 weeks was the best method for shoots induction (14 plantlets per 3 g of somatic embryos) of three rice varieties.
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Claims 3
Claim · model-written summary
The optimized MS-B5 medium produced 89% embryogenic callus without browning.
- Taxon
- rice
Oryza sativae1 - Treatment
- Optimised MS-B5 medium containing 1 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 10 mg/L NAA (α-naphthaleneacetic acid)e0
- Outcome
- production of embryogenic calluse0
- Direction
- higheste0
- Value
- 89%e0
- Qualifier
- without any browning effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimised MS-B5 medium containing 1 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 10 mg/L NAA (α-naphthaleneacetic acid) produced highest production of embryogenic callus (89%) without any browning effect”
e1“three aromatic rice varieties”
Claim · model-written summary
ABA and Gelrite incubation produced the highest somatic embryo frequency, 75%.
- Taxon
- riceOryza sativae1
- Treatment
- incubating embryogenic calli on same media containing 10 mg/L ABA and 9 g/L Gelrite agar for 8 weekse0
- Outcome
- whitish somatic embryos frequencye0
- Direction
- higheste0
- Value
- 75 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest whitish somatic embryos frequency (75 %) was initiated by incubating embryogenic calli on same media containing 10 mg/L ABA and 9 g/L Gelrite agar for 8 weeks”
e1“three aromatic rice varieties”
Claim · model-written summary
The described pretreatment and regeneration medium gave 14 plantlets per 3 g of somatic embryos.
- Taxon
- riceOryza sativae0
- Treatment
- medium containing NAA and 2,4-D which was previously pre-treated with 10 mg/L ABA, 9 g/L Gelrite agar and incubation at 8 weekse0
- Outcome
- numbers of regenerated plantletse0
- Direction
- beste0
- Value
- 14 plantlets per 3 g of somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the numbers of regenerated plantlets on medium containing NAA and 2,4-D which was previously pre-treated with 10 mg/L ABA, 9 g/L Gelrite agar and incubation at 8 weeks was the best method for shoots induction (14 plantlets per 3 g of somatic embryos) of three rice varieties”
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