Abstract
Seed vigour is an important trait for direct seeding in rice. In this study, indole-3-acetate beta-glucosyltransferase OsIAGLU was cloned in rice, and its roles on seed vigour were mainly investigated. Disruption of OsIAGLU resulted in low seed vigour in rice. Quantitative RT-PCR analysis showed that the expressions of OsIAGLU were relatively higher in the late developing and the early germinating seeds and were significantly induced by indole-3-acetic acid (IAA) and abscisic acid (ABA). Transcriptome analysis revealed that the IAA- and ABA-related genes were involved in the OsIAGLU regulation of seed vigour in rice. The higher levels of free IAA and ABA were identified in germinating seeds of osiaglu mutants compared to wild-type (WT) plants. When treated with exogenous IAA and ABA, the osiaglu mutants and WT plants showed sensitivity to ABA while not IAA, but the exogenous IAA amplified ABA-induced reduction of seed vigour in rice. The continuously higher expressions of ABA-INSENSITIVE 3 (OsABI3) and OsABI5 occurred in germinating seeds of osiaglu mutants compared to WT plants. The regulation of seed vigour by OsIAGLU might be through modulating IAA and ABA levels to alert OsABIs expression in germinating seeds in rice. Based on analysis of single-nucleotide polymorphism data of rice accessions, two haplotypes of OsIAGLU that positively correlated with seed vigour were identified in indica accessions. This study provides important insights into the roles of OsIAGLU on seed vigour and facilitates the practical use of OsIAGLU in rice breeding.
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Claims 15
Claim · model-written summary
OsIAGLU was cloned in rice.
- Taxon
- rice
Oryza sativae0 - Gene
- OsIAGLUe0
- Outcome
- clonede0
- Value
- OsIAGLUe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indole-3-acetate beta-glucosyltransferase OsIAGLU was cloned in rice”
Claim · model-written summary
Disruption of OsIAGLU resulted in low rice seed vigour.
- Taxon
- riceOryza sativae0
- Treatment
- Disruption of OsIAGLUe0
- Gene
- OsIAGLUe0
- Outcome
- seed vigoure0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsIAGLU resulted in low seed vigour in rice.”
Claim · model-written summary
OsIAGLU expression was higher in late developing and early germinating seeds.
- Taxon
- riceOryza sativat0
- Organ / stage
- late developing and the early germinating seedse0
- Gene
- OsIAGLUe0
- Outcome
- expressions of OsIAGLUe0
- Direction
- relatively highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expressions of OsIAGLU were relatively higher in the late developing and the early germinating seeds”
t0“rice”
Claim · model-written summary
IAA significantly induced OsIAGLU expression.
- Taxon
- riceOryza sativat0
- Treatment
- indole-3-acetic acid (IAA)e0
- Gene
- OsIAGLUe0
- Outcome
- expressions of OsIAGLUe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expressions of OsIAGLU were relatively higher in the late developing and the early germinating seeds and were significantly induced by indole-3-acetic acid (IAA) and abscisic acid (ABA).”
t0“rice”
Claim · model-written summary
ABA significantly induced OsIAGLU expression.
- Taxon
- riceOryza sativat0
- Treatment
- abscisic acid (ABA)e0
- Gene
- OsIAGLUe0
- Outcome
- expressions of OsIAGLUe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expressions of OsIAGLU were relatively higher in the late developing and the early germinating seeds and were significantly induced by indole-3-acetic acid (IAA) and abscisic acid (ABA).”
t0“rice”
Claim · model-written summary
IAA and ABA related genes were implicated in OsIAGLU regulation of seed vigour.
- Taxon
- riceOryza sativae0
- Gene
- OsIAGLUe0
- Outcome
- seed vigoure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the IAA- and ABA-related genes were involved in the OsIAGLU regulation of seed vigour in rice.”
Claim · model-written summary
Free IAA was higher in germinating mutant seeds than wild type.
- Taxon
- riceOryza sativat0
- Material
- osiaglu mutantse0
- Organ / stage
- germinating seedse0
- Outcome
- free IAAe0
- Direction
- highere0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The higher levels of free IAA and ABA were identified in germinating seeds of osiaglu mutants compared to wild-type (WT) plants.”
t0“rice”
Claim · model-written summary
ABA was higher in germinating mutant seeds than wild type.
- Taxon
- riceOryza sativat0
- Material
- osiaglu mutantse0
- Organ / stage
- germinating seedse0
- Outcome
- ABAe0
- Direction
- highere0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The higher levels of free IAA and ABA were identified in germinating seeds of osiaglu mutants compared to wild-type (WT) plants.”
t0“rice”
Claim · model-written summary
Mutants and wild type plants showed sensitivity to ABA.
- Taxon
- riceOryza sativat0
- Treatment
- ABAe0
- Outcome
- sensitivity to ABAe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the osiaglu mutants and WT plants showed sensitivity to ABA while not IAA”
t0“rice”
Claim · model-written summary
Mutants and wild type plants did not show sensitivity to IAA.
- Taxon
- riceOryza sativat0
- Treatment
- IAAe0
- Outcome
- sensitivity to ABA while not IAAe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the osiaglu mutants and WT plants showed sensitivity to ABA while not IAA”
t0“rice”
Claim · model-written summary
Exogenous IAA amplified ABA-induced reduction of seed vigour.
- Taxon
- riceOryza sativae0
- Treatment
- exogenous IAAe0
- Outcome
- seed vigoure0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the exogenous IAA amplified ABA-induced reduction of seed vigour in rice.”
Claim · model-written summary
OsABI3 expression remained higher in mutant germinating seeds than wild type.
- Taxon
- riceOryza sativat0
- Material
- osiaglu mutantse0
- Organ / stage
- germinating seedse0
- Gene
- OsABI3e0
- Outcome
- expressions of ABA-INSENSITIVE 3 (OsABI3)e0
- Direction
- highere0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The continuously higher expressions of ABA-INSENSITIVE 3 (OsABI3) and OsABI5 occurred in germinating seeds of osiaglu mutants compared to WT plants.”
t0“rice”
Claim · model-written summary
OsABI5 expression remained higher in mutant germinating seeds than wild type.
- Taxon
- riceOryza sativat0
- Material
- osiaglu mutantse0
- Organ / stage
- germinating seedse0
- Gene
- OsABI5e0
- Outcome
- OsABI5e0
- Direction
- highere0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The continuously higher expressions of ABA-INSENSITIVE 3 (OsABI3) and OsABI5 occurred in germinating seeds of osiaglu mutants compared to WT plants.”
t0“rice”
Claim · model-written summary
OsIAGLU may regulate seed vigour through IAA and ABA levels and OsABIs expression.
- Taxon
- riceOryza sativae0
- Gene
- OsIAGLUe0
- Outcome
- seed vigoure0
- Qualifier
- might be through modulating IAA and ABA levels to alert OsABIs expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regulation of seed vigour by OsIAGLU might be through modulating IAA and ABA levels to alert OsABIs expression in germinating seeds in rice.”
Claim · model-written summary
Two OsIAGLU haplotypes correlated positively with seed vigour in indica accessions.
- Taxon
- riceOryza sativae1
- Material
- indica accessionse0
- Gene
- OsIAGLUe0
- Outcome
- seed vigoure0
- Direction
- positively correlatede0
- Value
- two haplotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two haplotypes of OsIAGLU that positively correlated with seed vigour were identified in indica accessions.”
e1“rice accessions”
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