Abstract
[Objective]To explore the effect of auxin(IAA) and its polar auxin transport carriers on the formation of rice root hair and genetic expression of aquaporins.[Method]The varieties of wild-type Japonica rice Zhonghua11 and its over-expressed OsPIN1 a transgenic rice primary root were used in this paper.A small agar block which contains different concentrations of IAA and combination of IAA and inhibitors of polar auxin transport efflux carrier TIBA(2,3,5-Triiodobenzoic acid),NPA(N-1-naphthylphthalamic acid) or polar auxin transport influx carrier CHPAA(3-Chloro-4-hydroxyphenylacetic acid) was affixed to the lateral side of rice root tip when the primary root reached 0.5—1.0 cm in length in a aeroponic and dark culture.After a 12 h treatment,the root hair length,root growth rate,root hair density and relative water content in rice roots were detected.The photos were taken by confocal laser scanning microscope(Zeiss Axiophot microscope with a Qimaging Retiga 1300 12-bit monochrome CCD camera with Open Lab v3.0.9) to observe subcellular localization of OsPIN1a-GFP in the tip and hair of the rice root.In addition,semi-quantitative RT-PCR was applied to detecting the OsPIN1 a and aquaporins genes expression before and after the formation of the rice root hair.[Result]The results showed that the length and density of the root hair enhanced correspondingly with an increase in IAA concentration range from 0 to 5.0 mg·L-1,and 2.5 mg·L-1 IAA had an optimal effect on inducing the formation of root hair.However,as IAA exceeds 5.0 mg·L-1,the increase in root hair was insignificant and thus the primary root growth was severely inhibited.The new root hair only appeared when agar was attached to the meristematic zone of root tip but not formed in the ripe areas of the primary root.Combined treatment with IAA and lower concentration of TIBA or NPA did not affect formation of root hairs.In contrast,higher concentration of TIBA or NPA significantly inhibited its formation and elongation.However,much lower concentrations of CHPAA can significantly inhibit the formation and elongation of the root hair.In addition,IAA treatment increased genes expression of aquaporins as well as the formation of the root hair,thus improving the relative content of water in root tips.IAA treatment markedly improved the expression of OsPIN1 a and the content of OsPIN1a-GFP in root tips and root hair.[Conclusion]It was suggested that the formation of rice root hair was induced by IAA and associated with the involvement of IAA efflux and influx transporters and influx process had the most significant effect.OsPIN1 a played a key role in the formation and elongation of the root hair.IAA can promote genes expression of aquaporins and increase relative water content,thus alleviating water stress in rice roots in the aeroponic culture.
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Claims 10
Claim · model-written summary
Root hair length and density increased with IAA from 0 to 5.0 mg·L-1.
- Taxon
- Rice
Oryza sativat0 - Treatment
- IAAe0
- Outcome
- length and density of the root haire0
- Direction
- enhancede0
- Value
- 0 to 5.0 mg·L-1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the length and density of the root hair enhanced correspondingly with an increase in IAA concentration range from 0 to 5.0 mg·L-1”
t0“Rice”
Claim · model-written summary
IAA at 2.5 mg·L-1 optimally induced root hairs.
- Taxon
- RiceOryza sativat0
- Treatment
- 2.5 mg·L-1 IAAe0
- Outcome
- formation of root haire0
- Direction
- optimale0
- Value
- 2.5 mg·L-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.5 mg·L-1 IAA had an optimal effect on inducing the formation of root hair”
t0“Rice”
Claim · model-written summary
IAA above 5.0 mg·L-1 insignificantly increased root hairs and severely inhibited primary root growth.
- Taxon
- RiceOryza sativat0
- Treatment
- IAA exceeds 5.0 mg·L-1e0
- Outcome
- primary root growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as IAA exceeds 5.0 mg·L-1,the increase in root hair was insignificant and thus the primary root growth was severely inhibited”
t0“Rice”
Claim · model-written summary
New root hairs appeared only with agar at the root tip meristematic zone.
- Taxon
- RiceOryza sativat0
- Organ / stage
- meristematic zone of root tipe0
- Outcome
- new root haire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The new root hair only appeared when agar was attached to the meristematic zone of root tip but not formed in the ripe areas of the primary root”
t0“Rice”
Claim · model-written summary
Low TIBA or NPA with IAA did not affect root hair formation.
- Taxon
- RiceOryza sativat0
- Treatment
- IAA and lower concentration of TIBA or NPAe0
- Outcome
- formation of root hairse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combined treatment with IAA and lower concentration of TIBA or NPA did not affect formation of root hairs”
t0“Rice”
Claim · model-written summary
Higher TIBA or NPA inhibited root hair formation and elongation.
- Taxon
- RiceOryza sativat0
- Treatment
- higher concentration of TIBA or NPAe0
- Outcome
- formation and elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher concentration of TIBA or NPA significantly inhibited its formation and elongation”
t0“Rice”
Claim · model-written summary
Lower CHPAA concentrations inhibited root hair formation and elongation.
- Taxon
- RiceOryza sativat0
- Treatment
- much lower concentrations of CHPAAe0
- Outcome
- formation and elongation of the root haire0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“much lower concentrations of CHPAA can significantly inhibit the formation and elongation of the root hair”
t0“Rice”
Claim · model-written summary
IAA increased aquaporin gene expression.
- Taxon
- RiceOryza sativat0
- Treatment
- IAA treatmente0
- Gene
- aquaporinse0
- Outcome
- genes expression of aquaporinse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA treatment increased genes expression of aquaporins”
t0“Rice”
Claim · model-written summary
IAA increased relative water content in root tips.
- Taxon
- RiceOryza sativat0
- Treatment
- IAA treatmente0
- Outcome
- relative content of water in root tipse0
- Direction
- improvinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA treatment increased genes expression of aquaporins as well as the formation of the root hair,thus improving the relative content of water in root tips”
t0“Rice”
Claim · model-written summary
IAA increased OsPIN1 a expression and OsPIN1a-GFP content.
- Taxon
- RiceOryza sativat0
- Treatment
- IAA treatmente0
- Gene
- OsPIN1 ae0
- Outcome
- expression of OsPIN1 a and the content of OsPIN1a-GFPe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA treatment markedly improved the expression of OsPIN1 a and the content of OsPIN1a-GFP in root tips and root hair”
t0“Rice”
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