Abstract
Rice bZIP transcription factors mediate abiotic stress responses, but OsbZIP35’s function remains unclear. Here, we investigated its role in drought stress and yield regulation. In this study, drought, H2O2, abscisic acid, and NaCl treatments strongly induced the expression of OsbZIP35, whereas treatment with gibberellin, indoleacetic acid, and jasmonic acid did not affect OsbZIP35 expression. Using knockout mutants (bzip35) and overexpression lines (OsbZIP35-OX) for reciprocal validation, we found bzip35 mutants are drought-sensitive, while OsbZIP35-OX lines show enhanced tolerance across during the germination, post-germination growth, and seedling stages. Specifically, the bzip35 mutants exhibited a lower germination percentage, a weaker growth phenotype, and an increase in reactive oxygen species accumulation. Further analysis indicated that, under drought stress, OsbZIP35 regulated reactive oxygen species accumulation by modulating the contents of antioxidants, thereby positively regulating the response of rice seedlings to drought stress. In addition, we observed that OsbZIP35 could be phosphorylated by OsSAPK3 and participated in the abscisic acid signaling pathway in response to drought stress. Agronomic trait analysis revealed that, under drought stress at the heading stage, the panicle length and seed-set rate of bzip35 mutants were significantly lower than those of the wild type. This study examined the role of OsbZIP35 in rice stress tolerance and yield regulation, and identified an upstream regulatory gene, OsSAPK3.
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Claims 12
Claim · model-written summary
Drought, H2O2, abscisic acid and NaCl induced OsbZIP35 expression.
- Taxon
- Rice
Oryza sativat0 - Treatment
- drought, H2O2, abscisic acid, and NaCl treatmentse0
- Gene
- OsbZIP35e0
- Outcome
- expression of OsbZIP35e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drought, H2O2, abscisic acid, and NaCl treatments strongly induced the expression of OsbZIP35”
t0“Rice”
Claim · model-written summary
Gibberellin, indoleacetic acid and jasmonic acid did not affect OsbZIP35 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- gibberellin, indoleacetic acid, and jasmonic acide0
- Gene
- OsbZIP35e0
- Outcome
- OsbZIP35 expressione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with gibberellin, indoleacetic acid, and jasmonic acid did not affect OsbZIP35 expression.”
t0“Rice”
Claim · model-written summary
bzip35 knockout mutants were drought sensitive.
- Taxon
- RiceOryza sativat0
- Material
- bzip35 mutantse0
- Outcome
- drought-sensitivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we found bzip35 mutants are drought-sensitive”
t0“Rice”
Claim · model-written summary
OsbZIP35 overexpression lines showed enhanced drought tolerance across early stages.
- Taxon
- RiceOryza sativat0
- Material
- OsbZIP35-OX linese0
- Organ / stage
- germination, post-germination growth, and seedling stagese0
- Outcome
- tolerancee0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsbZIP35-OX lines show enhanced tolerance across during the germination, post-germination growth, and seedling stages.”
t0“Rice”
Claim · model-written summary
bzip35 mutants had lower germination percentage.
- Taxon
- RiceOryza sativat0
- Material
- bzip35 mutantse0
- Outcome
- germination percentagee0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bzip35 mutants exhibited a lower germination percentage, a weaker growth phenotype, and an increase in reactive oxygen species accumulation.”
t0“Rice”
Claim · model-written summary
bzip35 mutants had weaker growth.
- Taxon
- RiceOryza sativat0
- Material
- bzip35 mutantse0
- Outcome
- growth phenotypee0
- Direction
- weakere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bzip35 mutants exhibited a lower germination percentage, a weaker growth phenotype, and an increase in reactive oxygen species accumulation.”
t0“Rice”
Claim · model-written summary
bzip35 mutants accumulated more reactive oxygen species.
- Taxon
- RiceOryza sativat0
- Material
- bzip35 mutantse0
- Outcome
- reactive oxygen species accumulatione0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bzip35 mutants exhibited a lower germination percentage, a weaker growth phenotype, and an increase in reactive oxygen species accumulation.”
t0“Rice”
Claim · model-written summary
Under drought, OsbZIP35 regulated reactive oxygen species accumulation through antioxidant contents.
- Taxon
- riceOryza sativae0
- Gene
- OsbZIP35e0
- Outcome
- reactive oxygen species accumulatione0
- Qualifier
- modulating the contents of antioxidantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under drought stress, OsbZIP35 regulated reactive oxygen species accumulation by modulating the contents of antioxidants, thereby positively regulating the response of rice seedlings to drought stress.”
Claim · model-written summary
OsbZIP35 was phosphorylated by OsSAPK3 and participated in abscisic acid signalling under drought.
- Taxon
- RiceOryza sativat0
- Gene
- OsbZIP35e0
- Outcome
- phosphorylated by OsSAPK3 and participated in the abscisic acid signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsbZIP35 could be phosphorylated by OsSAPK3 and participated in the abscisic acid signaling pathway in response to drought stress.”
t0“Rice”
Claim · model-written summary
Under heading-stage drought, bzip35 mutants had significantly shorter panicles than wild type.
- Taxon
- RiceOryza sativat0
- Material
- bzip35 mutantse0
- Organ / stage
- heading stagee0
- Outcome
- panicle lengthe0
- Direction
- lowere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under drought stress at the heading stage, the panicle length and seed-set rate of bzip35 mutants were significantly lower than those of the wild type.”
t0“Rice”
Claim · model-written summary
Under heading-stage drought, bzip35 mutants had significantly lower seed set than wild type.
- Taxon
- RiceOryza sativat0
- Material
- bzip35 mutantse0
- Organ / stage
- heading stagee0
- Outcome
- seed-set ratee0
- Direction
- lowere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under drought stress at the heading stage, the panicle length and seed-set rate of bzip35 mutants were significantly lower than those of the wild type.”
t0“Rice”
Claim · model-written summary
OsSAPK3 was identified as an upstream regulatory gene.
- Taxon
- RiceOryza sativat0
- Gene
- OsSAPK3e0
- Outcome
- upstream regulatory genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified an upstream regulatory gene, OsSAPK3.”
t0“Rice”
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See all 45 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.