Abstract
Plant responses to auxin and phosphate (Pi) starvation are closely linked. However, the underlying mechanisms connecting auxin to phosphate starvation (-Pi) responses are largely unclear. Here, we show that OsARF16, an auxin response factor, functions in both auxin and -Pi responses in rice (Oryza sativa L.). The knockout of OsARF16 led to primary roots (PR), lateral roots (LR) and root hair losing sensitivity to auxin and -Pi response. OsARF16 expression and OsARF16::GUS staining in PR and LR of rice Nipponbare (NIP) were induced by indole acetic acid and -Pi treatments. In -Pi conditions, the shoot biomass of osarf16 was slightly reduced, and neither root growth nor iron content was induced, indicating that the knockout of OsARF16 led to loss of response to Pi deficiency in rice. Six phosphate starvation-induced genes (PSIs) were less induced by -Pi in osarf16 and these trends were similar to a knockdown mutant of OsPHR2 or AtPHR1, which was a key regulator under -Pi. These data first reveal the biological function of OsARF16, provide novel evidence of a linkage between auxin and -Pi responses and facilitate the development of new strategies for the efficient utilization of Pi in rice.
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Claims 8
Claim · model-written summary
OsARF16 knockout caused primary roots to lose sensitivity to auxin.
- Taxon
- rice (Oryza sativa L.)
Oryza sativae1 - Organ / stage
- primary roots (PR)e0
- Treatment
- knockout of OsARF16e0
- Gene
- OsARF16e0
- Outcome
- sensitivity to auxine0
- Direction
- losinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The knockout of OsARF16 led to primary roots (PR), lateral roots (LR) and root hair losing sensitivity to auxin and -Pi response.”
e1“in rice (Oryza sativa L.)”
Claim · model-written summary
OsARF16 knockout caused lateral roots and root hairs to lose sensitivity to auxin and phosphate starvation.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Organ / stage
- lateral roots (LR) and root haire0
- Treatment
- knockout of OsARF16e0
- Gene
- OsARF16e0
- Outcome
- sensitivity to auxin and -Pi responsee0
- Direction
- losinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The knockout of OsARF16 led to primary roots (PR), lateral roots (LR) and root hair losing sensitivity to auxin and -Pi response.”
e1“in rice (Oryza sativa L.)”
Claim · model-written summary
Indole acetic acid induced OsARF16 expression and reporter staining in Nipponbare primary and lateral roots.
- Taxon
- riceOryza sativae0
- Material
- Nipponbare (NIP)e0
- Organ / stage
- PR and LRe0
- Treatment
- indole acetic acide0
- Gene
- OsARF16e0
- Outcome
- OsARF16 expression and OsARF16::GUS staininge0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF16 expression and OsARF16::GUS staining in PR and LR of rice Nipponbare (NIP) were induced by indole acetic acid and -Pi treatments.”
Claim · model-written summary
Phosphate starvation induced OsARF16 expression and reporter staining in Nipponbare primary and lateral roots.
- Taxon
- riceOryza sativae0
- Material
- Nipponbare (NIP)e0
- Organ / stage
- PR and LRe0
- Treatment
- -Pi treatmentse0
- Gene
- OsARF16e0
- Outcome
- OsARF16 expression and OsARF16::GUS staininge0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF16 expression and OsARF16::GUS staining in PR and LR of rice Nipponbare (NIP) were induced by indole acetic acid and -Pi treatments.”
Claim · model-written summary
Under phosphate starvation, osarf16 shoot biomass was slightly reduced.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Material
- osarf16e0
- Organ / stage
- shoote0
- Treatment
- -Pi conditionse0
- Outcome
- shoot biomasse0
- Direction
- slightly reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In -Pi conditions, the shoot biomass of osarf16 was slightly reduced”
e1“in rice (Oryza sativa L.)”
Claim · model-written summary
Under phosphate starvation, osarf16 root growth was not induced.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Material
- osarf16e0
- Organ / stage
- roote0
- Treatment
- -Pi conditionse0
- Outcome
- root growthe0
- Direction
- nor iron content was inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In -Pi conditions, the shoot biomass of osarf16 was slightly reduced, and neither root growth nor iron content was induced”
e1“in rice (Oryza sativa L.)”
Claim · model-written summary
Under phosphate starvation, osarf16 iron content was not induced.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Material
- osarf16e0
- Treatment
- -Pi conditionse0
- Outcome
- iron contente0
- Direction
- was inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In -Pi conditions, the shoot biomass of osarf16 was slightly reduced, and neither root growth nor iron content was induced”
e1“in rice (Oryza sativa L.)”
Claim · model-written summary
Six phosphate starvation-induced genes were less induced by phosphate starvation in osarf16.
- Taxon
- rice (Oryza sativa L.)Oryza sativae1
- Material
- osarf16e0
- Treatment
- -Pie0
- Gene
- phosphate starvation-induced genes (PSIs)e0
- Outcome
- phosphate starvation-induced genes (PSIs)e0
- Direction
- less inducede0
- Value
- Sixe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six phosphate starvation-induced genes (PSIs) were less induced by -Pi in osarf16”
e1“in rice (Oryza sativa L.)”
The same questions in other papers
indole-3-acetic acid · molecular: expression · Oryza sativa (rice)Positive and negative results
11 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
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