Abstract
An efficient and reliable procedure for rooting and hardening of in vitro raised shoots of EMLA111 has been developed. Influence of different concentrations of IBA/NAA, solid/liquid medium, role of dark/light treatment and phloroglucinol (PG) was seen on rooting. Nearly 75% rooting was obtained on 1/2 MS with 0.1-0.5 mg/L IBA, 2% sucrose and 0.4% agar in one step method. However, successful rooting was related to the exposure of shoots to IBA for few days followed by transfer to auxin free media (two step method). A synergistic effect of IBA and phloroglucinol in light on rooting was observed. Rooted plantlets were successfully transferred to coco-peat for acclimatization. The hardened plants were transferred to open field conditions at different locations. Ninety-five to 100 percent success was found in March-April and September-November.
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Claims 7
Claim · model-written summary
The procedure for rooting and hardening in vitro shoots was described as efficient and reliable.
- Taxon
- APPLE
Malus domesticat0 - Material
- EMLA111e0
- Outcome
- efficient and reliable procedure for rooting and hardeninge0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“An efficient and reliable procedure for rooting and hardening of in vitro raised shoots of EMLA111 has been developed”
Claim · model-written summary
One step rooting reached nearly 75% on half strength MS with IBA, sucrose and agar.
- Taxon
- APPLEMalus domesticat0
- Material
- EMLA111t0
- Treatment
- 1/2 MS with 0.1-0.5 mg/L IBA, 2% sucrose and 0.4% agare0
- Outcome
- rootinge0
- Value
- 75%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“Nearly 75% rooting was obtained on 1/2 MS with 0.1-0.5 mg/L IBA, 2% sucrose and 0.4% agar in one step method”
Claim · model-written summary
Successful rooting was associated with brief IBA exposure followed by auxin free medium.
- Taxon
- APPLEMalus domesticat0
- Material
- EMLA111t0
- Treatment
- exposure of shoots to IBA for few days followed by transfer to auxin free mediae0
- Outcome
- successful rootinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“successful rooting was related to the exposure of shoots to IBA for few days followed by transfer to auxin free media (two step method)”
Claim · model-written summary
IBA and phloroglucinol acted synergistically on rooting in light.
- Taxon
- APPLEMalus domesticat0
- Treatment
- IBA and phloroglucinol in lighte0
- Outcome
- rootinge0
- Direction
- synergistice0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“A synergistic effect of IBA and phloroglucinol in light on rooting was observed”
Claim · model-written summary
Rooted plantlets were successfully acclimatized in coco-peat.
- Taxon
- APPLEMalus domesticat0
- Material
- EMLA111t0
- Outcome
- successfully transferred to coco-peat for acclimatizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“Rooted plantlets were successfully transferred to coco-peat for acclimatization”
Claim · model-written summary
Hardened plants were transferred to open field conditions.
- Taxon
- APPLEMalus domesticat0
- Outcome
- transferred to open field conditionse0
- Setting
- open field conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“The hardened plants were transferred to open field conditions at different locations”
Claim · model-written summary
Success in March-April and September-November was 95 to 100 percent.
- Taxon
- APPLEMalus domesticat0
- Outcome
- successe0
- Value
- Ninety-five to 100 percente0
- Qualifier
- March-April and September-Novembere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“APPLE ROOTSTOCK EMLA111”
e0“Ninety-five to 100 percent success was found in March-April and September-November”
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See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.