Abstract
This work was carried out to optimize a successful protocol for shoot rooting and acclimatization of tissue culture raised apple rootstocks of 'Malling' series. Root induction in dark in indole-3-butyric acid (IBA) containing MS medium and root elongation in medium without IBA provided the best results for in vitro rooting. Among various substrates tested for root elongation, agar was found better than perlite and sand. However, maximum rooting with good shoot quality was achieved, when liquid medium in place of agar solidified medium was used for root elongation. Rooted plantlets of about 5 cm long were subsequently transferred to different media for acclimatization. Plants grown in coco peat showed maximum survival as compared to those grown in soil containing medium. On the other hand, the in vitro elongated shoots could also be rooted ex vitro successfully in coco peat after inducing root initials in liquid medium in dark for simultaneous ex vitro root elongation and acclimatization. Nearly 95% hardening was achieved during the months of October to March in comparison to summer and rainy months. Plants transplanted to the field in March-April established more successfully as compared to rest of the period of the year. After six months, plants in the field showed satisfactory survival and growth. These plants were grafted with different varieties.
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Claims 8
Claim · model-written summary
In vitro rooting of apple rootstock shoots was best with root induction in dark on IBA-containing MS medium followed by root elongation without IBA.
- Taxon
- apple
Malus domesticae1 - Material
- Mallinge1
- Treatment
- Root induction in dark in indole-3-butyric acid (IBA) containing MS mediume2
- Outcome
- in vitro rootinge2
- Qualifier
- provided the best resultse2
- Setting
- in vitroe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e2“Root induction in dark in indole-3-butyric acid (IBA) containing MS medium and root elongation in medium without IBA provided the best results for in vitro rooting”
Claim · model-written summary
Agar was better than perlite and sand for root elongation of apple rootstocks.
- Taxon
- appleMalus domesticae1
- Treatment
- agare3
- Outcome
- root elongatione3
- Direction
- bettere3
- Comparator
- perlite and sande3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e3“Among various substrates tested for root elongation, agar was found better than perlite and sand”
Claim · model-written summary
Liquid medium in place of agar gave maximum rooting with good shoot quality in apple rootstocks.
- Taxon
- appleMalus domesticae1
- Treatment
- liquid medium in place of agar solidified mediume4
- Outcome
- rooting with good shoot qualitye4
- Direction
- maximume4
- Comparator
- agar solidified mediume4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e4“However, maximum rooting with good shoot quality was achieved, when liquid medium in place of agar solidified medium was used for root elongation”
Claim · model-written summary
Apple rootstock plants grown in coco peat showed maximum survival compared with soil-containing medium.
- Taxon
- appleMalus domesticae1
- Treatment
- coco peate5
- Outcome
- survivale5
- Direction
- maximume5
- Comparator
- soil containing mediume5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e5“Plants grown in coco peat showed maximum survival as compared to those grown in soil containing medium”
Claim · model-written summary
In vitro elongated apple rootstock shoots were rooted ex vitro successfully in coco peat after root initiation in liquid medium in dark.
- Taxon
- appleMalus domesticae1
- Treatment
- coco peate6
- Outcome
- rooted ex vitro successfullye6
- Qualifier
- after inducing root initials in liquid medium in darke6
- Setting
- ex vitroe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e6“the in vitro elongated shoots could also be rooted ex vitro successfully in coco peat after inducing root initials in liquid medium in dark”
Claim · model-written summary
Nearly 95% hardening of apple rootstocks was achieved from October to March, compared with summer and rainy months.
- Taxon
- appleMalus domesticae1
- Outcome
- hardeninge7
- Value
- Nearly 95%e7
- Qualifier
- during the months of October to Marche7
- Comparator
- summer and rainy monthse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e7“Nearly 95% hardening was achieved during the months of October to March in comparison to summer and rainy months”
Claim · model-written summary
Apple rootstock plants transplanted to the field in March–April established more successfully than in the rest of the year.
- Taxon
- appleMalus domesticae1
- Treatment
- transplanted to the field in March-Aprile2
- Outcome
- establishede2
- Direction
- more successfullye2
- Comparator
- rest of the period of the yeare2
- Setting
- fielde2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e2“Plants transplanted to the field in March-April established more successfully as compared to rest of the period of the year”
Claim · model-written summary
Apple rootstock plants in the field showed satisfactory survival and growth six months after transplanting.
- Taxon
- appleMalus domesticae1
- Outcome
- survival and growthe2
- Qualifier
- After six monthse2
- Setting
- fielde2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tissue culture raised apple rootstocks of 'Malling' series”
e2“After six months, plants in the field showed satisfactory survival and growth”
The same questions in other papers
agar · growth · Malus domestica (apple)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →growing medium · survival · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- positive
in vitro · quality: general · Malus domestica (apple)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.