Abstract
The effects of various factors including genotypes of donor plants, induction and differentiation media, and thermal shock pretreatment were evaluated on embryo-like structure (ELS) and callus formation in an unpollinated ovary culture of cucumber. All five cucumber cultivars, which were used as donor plants, produced ELSs and calli, although their ELS and callus formation potentials varied significantly. The addition of 1 mg/L thidiazuron (TDZ) and 1 mg/L 6-benzylaminopurine (BA) into the induction medium resulted in the highest percentage of ELS formation, ranging from 42.3 to 91.4% with an average of 60.4%. However, the highest percentage of callus formation was observed in an induction medium containing 2 mg/L BA, 0.5 mg/L indole-3-acetic acid (IAA), 1 mg/L gibberellic acid (GA 3 ) and 32 mg/L putrescine (70.8%). By contrast, differentiation media had no significant effect on the formation potentials of both ELSs and calli. Thermal shock pretreatment reduced the percentage of ELS formation ca. 1.3-fold, but had no significant effect on callus formation. These results can be implicated for the efficient production of cucumber doubled haploids in the future.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
All five cucumber cultivars produced embryo-like structures and calli in ovary culture
- Taxon
- cucumber
Cucumis sativuse0 - Material
- five cucumber cultivarse1
- Outcome
- ELSs and callie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e1“All five cucumber cultivars, which were used as donor plants, produced ELSs and calli, although their ELS and callus formation potentials varied significantly”
Claim · model-written summary
Embryo-like structure and callus formation potentials varied significantly among the five cucumber cultivars
- Taxon
- cucumberCucumis sativuse0
- Material
- five cucumber cultivarse1
- Outcome
- callus formation potentialse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e1“All five cucumber cultivars, which were used as donor plants, produced ELSs and calli, although their ELS and callus formation potentials varied significantly”
Claim · model-written summary
Adding 1 mg/L TDZ and 1 mg/L BA to the induction medium gave the highest ELS formation, averaging 60.4%
- Taxon
- cucumberCucumis sativuse0
- Treatment
- the addition of 1 mg/L thidiazuron (TDZ) and 1 mg/L 6-benzylaminopurine (BA) into the induction mediume2
- Outcome
- percentage of ELS formatione2
- Direction
- higheste2
- Value
- 60.4%e2
- Qualifier
- ranging from 42.3 to 91.4%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e2“The addition of 1 mg/L thidiazuron (TDZ) and 1 mg/L 6-benzylaminopurine (BA) into the induction medium resulted in the highest percentage of ELS formation, ranging from 42.3 to 91.4% with an average of 60.4%”
Claim · model-written summary
The induction medium with 2 mg/L BA, 0.5 mg/L IAA, 1 mg/L GA3 and 32 mg/L putrescine gave the highest callus formation, 70.8%
- Taxon
- cucumberCucumis sativuse0
- Treatment
- an induction medium containing 2 mg/L BA, 0.5 mg/L indole-3-acetic acid (IAA), 1 mg/L gibberellic acid (GA 3 ) and 32 mg/L putrescinee3
- Outcome
- percentage of callus formatione3
- Direction
- higheste3
- Value
- 70.8%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e3“However, the highest percentage of callus formation was observed in an induction medium containing 2 mg/L BA, 0.5 mg/L indole-3-acetic acid (IAA), 1 mg/L gibberellic acid (GA 3 ) and 32 mg/L putrescine (70.8%)”
Claim · model-written summary
Differentiation media had no significant effect on embryo-like structure and callus formation
- Taxon
- cucumberCucumis sativuse0
- Treatment
- differentiation mediae4
- Outcome
- formation potentials of both ELSs and callie4
- Direction
- no significant effecte4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e4“By contrast, differentiation media had no significant effect on the formation potentials of both ELSs and calli”
Claim · model-written summary
Thermal shock pretreatment reduced ELS formation about 1.3-fold
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Thermal shock pretreatmente5
- Outcome
- percentage of ELS formatione5
- Direction
- reducede5
- Value
- ca. 1.3-folde5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e5“Thermal shock pretreatment reduced the percentage of ELS formation ca. 1.3-fold, but had no significant effect on callus formation”
Claim · model-written summary
Thermal shock pretreatment had no significant effect on callus formation
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Thermal shock pretreatmente5
- Outcome
- callus formatione5
- Direction
- no significant effecte5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unpollinated ovary culture of cucumber”
e5“Thermal shock pretreatment reduced the percentage of ELS formation ca. 1.3-fold, but had no significant effect on callus formation”
Claim · model-written summary
The results can be applied to efficient production of cucumber doubled haploids
- Taxon
- cucumberCucumis sativuse6
- Outcome
- efficient production of cucumber doubled haploidse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These results can be implicated for the efficient production of cucumber doubled haploids in the future”
The same questions in other papers
indole-3-acetic acid · growth · Cucumis sativus (cucumber)Results differ
11 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- no effect
12 papers more in this group
See all 16 papers →N-benzyladenine · growth · Cucumis sativus (cucumber)Results differ
29 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- negative
30 papers more in this group
See all 34 papers →gibberellin A3 · growth · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- positive
putrescine · growth · Cucumis sativus (cucumber)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →in vitro · growth · Cucumis sativus (cucumber)Positive and negative results
23 papers in the same direction as this paper · 10 in the opposite direction
- negative
- negative
- negative
30 papers more in this group
See all 34 papers →in vitro · soil: physics · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.