Abstract
Changes in the rate of synthesis of cellular constituents were studied in detached cucumber cotyledons (Cucumis sativus L. cv. Ohio) after treatment with benzyladenine (BA). BA treatment stimulated RNA and DNA syntheses. The stimulation of RNA synthesis occurred within the lag phase preceding BA-induced growth, while that of DNA synthesis came during the growth. On the other hand, the stimulation by BA was not detected for protein and lipid syntheses. 5-Fluorouracil (FU, 10(-3) m) plus thymidine (T, 10(-4) m) did not inhibit the fresh weight increase in both water- and BA-treated cotyledons, while α-amanitin (AM, 10 µg/ml) inhibited it in both. AM added during the first 4 hr effectively inhibited the growth stimulation by BA, but AM added 6 hr or more after BA had almost no effect. FU+T strongly inhibited the incorporation of (3)H-uridine into cytoplasmic ribosomal RNA (rRNA) and brought about labelling in regions other than those of the regular rRNA species on gel electrophoresis. On the other hand, AM had less influence on the synthesis of cytoplasmic rRNA than FU+T. These results indicate that BA stimulates cotyledon growth in the dark through its effect on messenger RNA synthesis within the lag phase of growth and the synthesis of cytoplasmic rRNA is not always necessary for cotyledon growth.
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Claims 15
Claim · model-written summary
Benzyladenine stimulated RNA synthesis in cucumber cotyledons.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- BA treatmente0
- Outcome
- RNAe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BA treatment stimulated RNA and DNA syntheses.”
t0“cucumber cotyledons”
Claim · model-written summary
Benzyladenine stimulated DNA synthesis in cucumber cotyledons.
- Taxon
- cucumberCucumis sativust0
- Treatment
- BA treatmente0
- Outcome
- DNA synthesese0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BA treatment stimulated RNA and DNA syntheses.”
t0“cucumber cotyledons”
Claim · model-written summary
RNA synthesis stimulation preceded benzyladenine-induced growth.
- Taxon
- cucumberCucumis sativust0
- Outcome
- stimulation of RNA synthesis occurred within the lag phase preceding BA-induced growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The stimulation of RNA synthesis occurred within the lag phase preceding BA-induced growth”
t0“cucumber cotyledons”
Claim · model-written summary
DNA synthesis stimulation occurred during cotyledon growth.
- Taxon
- cucumberCucumis sativust0
- Outcome
- DNA synthesis came during the growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while that of DNA synthesis came during the growth.”
t0“cucumber cotyledons”
Claim · model-written summary
BA did not detectably stimulate protein synthesis.
- Taxon
- cucumberCucumis sativust0
- Treatment
- BAe0
- Outcome
- proteine0
- Direction
- not detectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stimulation by BA was not detected for protein and lipid syntheses.”
t0“cucumber cotyledons”
Claim · model-written summary
BA did not detectably stimulate lipid synthesis.
- Taxon
- cucumberCucumis sativust0
- Treatment
- BAe0
- Outcome
- lipid synthesese0
- Direction
- not detectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stimulation by BA was not detected for protein and lipid syntheses.”
t0“cucumber cotyledons”
Claim · model-written summary
FU plus thymidine did not inhibit fresh-weight gain in water- or BA-treated cotyledons.
- Taxon
- cucumberCucumis sativust0
- Treatment
- 5-Fluorouracil (FU, 10(-3) m) plus thymidine (T, 10(-4) m)e0
- Outcome
- fresh weight increasee0
- Direction
- did not inhibite0
- Comparator
- water- and BA-treated cotyledonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5-Fluorouracil (FU, 10(-3) m) plus thymidine (T, 10(-4) m) did not inhibit the fresh weight increase in both water- and BA-treated cotyledons”
t0“cucumber cotyledons”
Claim · model-written summary
Alpha-amanitin inhibited fresh-weight increase in water- and BA-treated cotyledons.
- Taxon
- cucumberCucumis sativust0
- Treatment
- α-amanitin (AM, 10 µg/ml)e0
- Outcome
- fresh weight increasee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5-Fluorouracil (FU, 10(-3) m) plus thymidine (T, 10(-4) m) did not inhibit the fresh weight increase in both water- and BA-treated cotyledons, while α-amanitin (AM, 10 µg/ml) inhibited it in both.”
t0“cucumber cotyledons”
Claim · model-written summary
Alpha-amanitin during the first 4 hours inhibited BA growth stimulation.
- Taxon
- cucumberCucumis sativust0
- Treatment
- AM added during the first 4 hre0
- Outcome
- growth stimulation by BAe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AM added during the first 4 hr effectively inhibited the growth stimulation by BA”
t0“cucumber cotyledons”
Claim · model-written summary
Alpha-amanitin added after 6 hours had almost no effect on BA growth stimulation.
- Taxon
- cucumberCucumis sativust0
- Treatment
- AM added 6 hr or more after BAe0
- Outcome
- growth stimulatione0
- Direction
- almost no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AM added during the first 4 hr effectively inhibited the growth stimulation by BA, but AM added 6 hr or more after BA had almost no effect.”
t0“cucumber cotyledons”
Claim · model-written summary
FU plus thymidine strongly inhibited uridine incorporation into cytoplasmic rRNA.
- Taxon
- cucumberCucumis sativust0
- Treatment
- FU+Te0
- Outcome
- incorporation of (3)H-uridine into cytoplasmic ribosomal RNA (rRNA)e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FU+T strongly inhibited the incorporation of (3)H-uridine into cytoplasmic ribosomal RNA (rRNA)”
t0“cucumber cotyledons”
Claim · model-written summary
FU plus thymidine produced labeling outside regular rRNA regions.
- Taxon
- cucumberCucumis sativust0
- Treatment
- FU+Te0
- Outcome
- labelling in regions other than those of the regular rRNA speciese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FU+T strongly inhibited the incorporation of (3)H-uridine into cytoplasmic ribosomal RNA (rRNA) and brought about labelling in regions other than those of the regular rRNA species on gel electrophoresis.”
t0“cucumber cotyledons”
Claim · model-written summary
Alpha-amanitin influenced cytoplasmic rRNA synthesis less than FU plus thymidine.
- Taxon
- cucumberCucumis sativust0
- Treatment
- AMe0
- Outcome
- synthesis of cytoplasmic rRNAe0
- Direction
- less influencee0
- Comparator
- FU+Te0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AM had less influence on the synthesis of cytoplasmic rRNA than FU+T.”
t0“cucumber cotyledons”
Claim · model-written summary
The authors infer that BA stimulates dark cotyledon growth through messenger RNA synthesis during the lag phase.
- Taxon
- cucumberCucumis sativust0
- Treatment
- BAe0
- Outcome
- cotyledon growth in the darke0
- Qualifier
- through its effect on messenger RNA synthesis within the lag phase of growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BA stimulates cotyledon growth in the dark through its effect on messenger RNA synthesis within the lag phase of growth”
t0“cucumber cotyledons”
Claim · model-written summary
The authors conclude that cytoplasmic rRNA synthesis is not always necessary for cotyledon growth.
- Taxon
- cucumberCucumis sativust0
- Outcome
- cotyledon growthe0
- Qualifier
- synthesis of cytoplasmic rRNA is not always necessarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the synthesis of cytoplasmic rRNA is not always necessary for cotyledon growth.”
t0“cucumber cotyledons”
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See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.