Abstract
In plants, low fluences of ultraviolet-B radiation (UV-B; 280-320 nm) trigger the activation of protection mechanisms and various photomorphogenic responses. We established a novel screen to isolate Arabidopsis T-DNA mutants (uli, UV-B light insensitive) with reduced sensitivity towards UV-B light-mediated inhibition of hypocotyl growth. One of the mutants, uli3, shows also a reduced sensitivity of UV-B-induced gene expression events and was, therefore, chosen for further investigation. The ULI3 gene encodes an 80-kDa protein with potential domains for heme- and diacylglycerol-binding. In transiently transfected protoplasts, the ULI3:GFP fusion protein is localised in the cytoplasm but also adjacent to membranes. In etiolated Arabidopsis seedlings irradiated by UV-B, ULI3 mRNA expression is strongly up-regulated. This is caused by elevated transcription as demonstrated using stable transformants where a GUS-reporter was driven by the ULI3-promoter. ULI3 is preferentially expressed in the outer cell layers in leaves, stems and flowers, but not in roots. There is evidence that ULI3 represents a specific component involved in UV-B-mediated signal transduction in higher plants.
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Claims 8
Claim · model-written summary
A screen isolated Arabidopsis T-DNA uli mutants with reduced sensitivity to UV-B light-mediated inhibition of hypocotyl growth.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- T-DNA mutantse0
- Organ / stage
- hypocotyle0
- Treatment
- UV-B lighte0
- Outcome
- reduced sensitivity towards UV-B light-mediated inhibition of hypocotyl growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We established a novel screen to isolate Arabidopsis T-DNA mutants (uli, UV-B light insensitive) with reduced sensitivity towards UV-B light-mediated inhibition of hypocotyl growth”
Claim · model-written summary
The uli3 mutant also showed reduced sensitivity of UV-B-induced gene expression events.
- Taxon
- ArabidopsisArabidopsist0
- Material
- uli3e0
- Outcome
- reduced sensitivity of UV-B-induced gene expression eventse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the mutants, uli3, shows also a reduced sensitivity of UV-B-induced gene expression events”
t0“Arabidopsis”
Claim · model-written summary
The ULI3 gene encodes an 80-kDa protein with potential heme- and diacylglycerol-binding domains.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ULI3e0
- Outcome
- 80-kDa protein with potential domains for heme- and diacylglycerol-bindinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ULI3 gene encodes an 80-kDa protein with potential domains for heme- and diacylglycerol-binding”
t0“Arabidopsis”
Claim · model-written summary
In transiently transfected protoplasts the ULI3:GFP fusion protein was localised in the cytoplasm and also adjacent to membranes.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- protoplastse0
- Gene
- ULI3:GFPe0
- Outcome
- localised in the cytoplasm but also adjacent to membranese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transiently transfected protoplasts, the ULI3:GFP fusion protein is localised in the cytoplasm but also adjacent to membranes”
t0“Arabidopsis”
Claim · model-written summary
In etiolated Arabidopsis seedlings irradiated by UV-B, ULI3 mRNA expression was strongly up-regulated.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- seedlingse0
- Treatment
- irradiated by UV-Be0
- Gene
- ULI3e0
- Outcome
- ULI3 mRNA expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In etiolated Arabidopsis seedlings irradiated by UV-B, ULI3 mRNA expression is strongly up-regulated”
Claim · model-written summary
The UV-B up-regulation of ULI3 mRNA is attributed to elevated transcription, shown with a ULI3-promoter GUS reporter in stable transformants.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ULI3-promotere1
- Outcome
- ULI3 mRNA expressione0
- Qualifier
- elevated transcriptione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In etiolated Arabidopsis seedlings irradiated by UV-B, ULI3 mRNA expression is strongly up-regulated”
e1“This is caused by elevated transcription as demonstrated using stable transformants where a GUS-reporter was driven by the ULI3-promoter”
Claim · model-written summary
ULI3 is preferentially expressed in the outer cell layers of leaves, stems and flowers but not in roots.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- outer cell layers in leaves, stems and flowers, but not in rootse0
- Gene
- ULI3e0
- Outcome
- preferentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ULI3 is preferentially expressed in the outer cell layers in leaves, stems and flowers, but not in roots”
t0“Arabidopsis”
Claim · model-written summary
The authors conclude there is evidence that ULI3 is a specific component of UV-B-mediated signal transduction in higher plants.
- Taxon
- higher plantsEmbryophytae0
- Gene
- ULI3e0
- Outcome
- UV-B-mediated signal transductione0
Verbatim
e0“There is evidence that ULI3 represents a specific component involved in UV-B-mediated signal transduction in higher plants”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.