Abstract
UV and blue light stimulate transcription of key flavonoid biosynthesis genes in a range of higher plants. Here, we provide evidence that several distinct "inductive" and "synergistic" UV/blue phototransduction pathways regulate chalcone synthase (CHS) gene transcription and transcript accumulation in Arabidopsis leaf tissue. Experiments with the long-hypocotyl hy4-2.23N mutant showed that separate inductive pathways mediate responses to UV-B and UV-A/blue light. Only the UV-A/blue light induction of CHS expression involved the CRY1 photoreceptor. In addition, UV-A and blue light each act synergistically with UV-B to stimulate CHS transcript accumulation and beta-glucuronidase activity driven by a CHS promoter in transgenic leaf tissue. The UV-A and blue phototransduction pathways responsible for synergism are distinct because they produce transient and relatively stable signals, respectively, and can function additively to stimulate CHS promoter function. The hy4-2.23N mutant retains the synergistic interactions between UV-B and both UV-A and blue light, indicating that neither synergism pathway involves the CRY1 photoreceptor. Our findings reveal considerable complexity in both photoreception and signal transduction in regulating CHS gene expression by UV and blue light.
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Claims 8
Claim · model-written summary
Distinct inductive pathways mediate UV-B and UV-A/blue responses.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- hy4-2.23N mutante0
- Treatment
- UV-B and UV-A/blue lighte0
- Outcome
- separate inductive pathways mediate responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experiments with the long-hypocotyl hy4-2.23N mutant showed that separate inductive pathways mediate responses to UV-B and UV-A/blue light.”
t0“Arabidopsis”
Claim · model-written summary
CRY1 participates only in UV-A/blue induction of CHS expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-A/blue lighte0
- Gene
- CRY1 photoreceptore0
- Outcome
- induction of CHS expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only the UV-A/blue light induction of CHS expression involved the CRY1 photoreceptor.”
t0“Arabidopsis”
Claim · model-written summary
UV-A acts synergistically with UV-B to stimulate CHS transcripts and promoter-driven GUS activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Ae0
- Gene
- CHSe0
- Outcome
- CHS transcript accumulation and beta-glucuronidase activitye0
- Direction
- stimulatee0
- Comparator
- UV-Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-A and blue light each act synergistically with UV-B to stimulate CHS transcript accumulation and beta-glucuronidase activity driven by a CHS promoter in transgenic leaf tissue.”
t0“Arabidopsis”
Claim · model-written summary
Blue light acts synergistically with UV-B to stimulate CHS transcripts and promoter-driven GUS activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- blue lighte0
- Gene
- CHSe0
- Outcome
- CHS transcript accumulation and beta-glucuronidase activitye0
- Direction
- stimulatee0
- Comparator
- UV-Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-A and blue light each act synergistically with UV-B to stimulate CHS transcript accumulation and beta-glucuronidase activity driven by a CHS promoter in transgenic leaf tissue.”
t0“Arabidopsis”
Claim · model-written summary
UV-A produces transient synergistic signals.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Ae0
- Outcome
- transiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The UV-A and blue phototransduction pathways responsible for synergism are distinct because they produce transient and relatively stable signals, respectively”
t0“Arabidopsis”
Claim · model-written summary
Blue light produces relatively stable synergistic signals.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- bluee0
- Outcome
- relatively stable signalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The UV-A and blue phototransduction pathways responsible for synergism are distinct because they produce transient and relatively stable signals, respectively”
t0“Arabidopsis”
Claim · model-written summary
UV-A and blue pathways can additively stimulate CHS promoter function.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CHSe0
- Outcome
- CHS promoter functione0
- Direction
- stimulatee0
- Qualifier
- UV-A and blue phototransduction pathwayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can function additively to stimulate CHS promoter function.”
e1“The UV-A and blue phototransduction pathways”
t0“Arabidopsis”
Claim · model-written summary
The hy4 mutant retains UV-B/UV-A and UV-B/blue synergism, suggesting CRY1 is uninvolved in either.
- Taxon
- ArabidopsisArabidopsist0
- Material
- hy4-2.23N mutante0
- Gene
- CRY1 photoreceptore0
- Outcome
- synergistic interactions between UV-B and both UV-A and blue lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hy4-2.23N mutant retains the synergistic interactions between UV-B and both UV-A and blue light, indicating that neither synergism pathway involves the CRY1 photoreceptor.”
t0“Arabidopsis”
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See all 80 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.