Abstract
Ultraviolet-B light (UV-B) regulates the expression of genes in a wavelength- and fluence rate-dependent fashion. A signaling pathway consisting of CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) and UV RESISTANCE LOCUS 8 (UVR 8) mediates responsiveness to longer wavelength, low intensity UV-B light-activating, for example, HY5 gene expression. By contrast, transcription of another group of genes, including ANAC13, modulated by shorter wavelength, higher intensity UV-B is controlled by a yet unknown and largely COP1-independent signaling cascade. Here we provide evidence by promoter deletion analysis, and characterization of genetic mutants displaying aberrant expression patterns, that two cis-regulatory elements, designated MRE(ANAC13) and UVBox(ANAC13), are required for maximal UV-B induction of the ANAC13 gene in transgenic plants. These elements are located in the proximal 150-bp region of the ANAC13 promoter. They show no significant similarity to each other; the putative MRE(ANAC13) (-AACCTT-) is closely related to MRE(CHS) (-AACCTA-) found in the CHALCONE SYNTHASE (CHS) gene, whereas UVBox(ANAC13) (with core sequence CAAG) represents a novel cis-regulatory element. The novel UVBox(ANAC13) sequence is significantly enriched in the promoter region of a subset of UV-B-induced genes with similar activation properties as ANAC13. In addition, we demonstrate that expression of a chimeric gene containing only the dimerized 12-mer containing UVBox(ANAC13) fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses.
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Claims 6
Claim · model-written summary
Two cis-regulatory elements, MRE(ANAC13) and UVBox(ANAC13), are required for maximal UV-B induction of ANAC13 in transgenic Arabidopsis plants.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- ANAC13e1
- Outcome
- maximal UV-B induction of the ANAC13 genee1
- Qualifier
- MRE(ANAC13) and UVBox(ANAC13)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“two cis-regulatory elements, designated MRE(ANAC13) and UVBox(ANAC13), are required for maximal UV-B induction of the ANAC13 gene in transgenic plants”
t0“in Arabidopsis”
Claim · model-written summary
The UVBox(ANAC13) sequence is significantly enriched in promoters of a subset of UV-B-induced genes with activation properties similar to ANAC13.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- UV-B-induced genese2
- Outcome
- UVBox(ANAC13) sequencee2
- Direction
- enrichede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The novel UVBox(ANAC13) sequence is significantly enriched in the promoter region of a subset of UV-B-induced genes”
t0“in Arabidopsis”
Claim · model-written summary
A chimeric reporter carrying the UVBox(ANAC13) 12-mer is efficiently induced by shorter wavelength, higher intensity UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- shorter wavelength, higher intensity UV-Be3
- Outcome
- expression of a chimeric genee3
- Direction
- inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“expression of a chimeric gene containing only the dimerized 12-mer containing UVBox(ANAC13) fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses”
t0“in Arabidopsis”
Claim · model-written summary
The chimeric reporter does not respond to longer wavelength UV-B and red light.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- longer wavelength UV-B and red lighte3
- Outcome
- expression of a chimeric genee3
- Direction
- does not responde3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“expression of a chimeric gene containing only the dimerized 12-mer containing UVBox(ANAC13) fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses”
t0“in Arabidopsis”
Claim · model-written summary
The chimeric reporter does not respond to abscisic acid treatment or osmotic and salt stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- abscisic acid treatment and osmotic, salte3
- Outcome
- expression of a chimeric genee3
- Direction
- does not responde3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“expression of a chimeric gene containing only the dimerized 12-mer containing UVBox(ANAC13) fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses”
t0“in Arabidopsis”
Claim · model-written summary
The chimeric reporter does not respond to heat and cold stresses.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- heat and cold stressese3
- Outcome
- expression of a chimeric genee3
- Direction
- does not responde3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“expression of a chimeric gene containing only the dimerized 12-mer containing UVBox(ANAC13) fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses”
t0“in Arabidopsis”
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