Abstract
Replication Protein A (RPA) is a heterotrimeric protein complex that binds single-stranded DNA. In plants, multiple genes encode the three RPA subunits (RPA1, RPA2 and RPA3), including five RPA1-like genes in Arabidopsis. Phylogenetic analysis suggests two distinct groups composed of RPA1A, RPA1C, RPA1E (ACE group) and RPA1B, RPA1D (BD group). ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ionizing radiation. Analysis of rpa1 T-DNA insertion mutants demonstrates that although each mutant line is likely null, all mutant lines are viable and display normal vegetative growth. The rpa1c and rpa1e single mutants however display hypersensitivity to ionizing radiation, and combination of rpa1c and rpa1e results in additive hypersensitivity to a variety of DNA damaging agents. Combination of the partially sterile rpa1a with rpa1c results in complete sterility, incomplete synapsis and meiotic chromosome fragmentation, suggesting an early role for RPA1C in promoting homologous recombination. Combination of either rpa1c and/or rpa1e with atr revealed additive hypersensitivity phenotypes consistent with each functioning in unique repair pathways. In contrast, rpa1b rpa1d double mutant plants display slow growth and developmental defects under non-damaging conditions. We show these defects in the rpa1b rpa1d mutant are likely the result of defective DNA replication leading to reduction in cell division.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Phylogeny separated RPA1-like genes into ACE and BD groups.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- RPA1A, RPA1C, RPA1Ee0
- Outcome
- two distinct groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis suggests two distinct groups composed of RPA1A, RPA1C, RPA1E (ACE group) and RPA1B, RPA1D (BD group).”
t0“Arabidopsis”
Claim · model-written summary
Ionizing radiation induced ACE-group transcription.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ionizing radiatione0
- Gene
- ACE-group memberse0
- Outcome
- transcriptionally inducede0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ionizing radiation.”
t0“Arabidopsis”
Claim · model-written summary
BD-group members had higher basal transcription and lacked radiation induction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- BD-group memberse0
- Outcome
- basal transcriptione0
- Direction
- highere0
- Comparator
- ACE-group memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ionizing radiation.”
t0“Arabidopsis”
Claim · model-written summary
All rpa1 insertion mutants were viable with normal vegetative growth.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1 T-DNA insertion mutantse0
- Outcome
- viable and display normal vegetative growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of rpa1 T-DNA insertion mutants demonstrates that although each mutant line is likely null, all mutant lines are viable and display normal vegetative growth.”
t0“Arabidopsis”
Claim · model-written summary
The rpa1c mutant was hypersensitive to ionizing radiation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1ce0
- Outcome
- hypersensitivity to ionizing radiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rpa1c and rpa1e single mutants however display hypersensitivity to ionizing radiation, and combination of rpa1c and rpa1e results in additive hypersensitivity to a variety of DNA damaging agents.”
t0“Arabidopsis”
Claim · model-written summary
The rpa1e mutant was hypersensitive to ionizing radiation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1ee0
- Outcome
- hypersensitivity to ionizing radiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rpa1c and rpa1e single mutants however display hypersensitivity to ionizing radiation, and combination of rpa1c and rpa1e results in additive hypersensitivity to a variety of DNA damaging agents.”
t0“Arabidopsis”
Claim · model-written summary
Combining rpa1c and rpa1e added hypersensitivity to DNA damaging agents.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1c and rpa1ee0
- Outcome
- additive hypersensitivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“combination of rpa1c and rpa1e results in additive hypersensitivity to a variety of DNA damaging agents.”
t0“Arabidopsis”
Claim · model-written summary
The rpa1a rpa1c combination caused complete sterility, incomplete synapsis and chromosome fragmentation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1a with rpa1ce0
- Outcome
- complete sterility, incomplete synapsis and meiotic chromosome fragmentatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combination of the partially sterile rpa1a with rpa1c results in complete sterility, incomplete synapsis and meiotic chromosome fragmentation, suggesting an early role for RPA1C in promoting homologous recombination.”
t0“Arabidopsis”
Claim · model-written summary
rpa1c and/or rpa1e with atr showed additive hypersensitivity.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1c and/or rpa1e with atre0
- Outcome
- additive hypersensitivity phenotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Combination of either rpa1c and/or rpa1e with atr revealed additive hypersensitivity phenotypes consistent with each functioning in unique repair pathways.”
t0“Arabidopsis”
Claim · model-written summary
rpa1b rpa1d plants grew slowly and had developmental defects in non-damaging conditions.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1b rpa1d double mutant plantse0
- Outcome
- slow growth and developmental defectse0
- Qualifier
- non-damaging conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rpa1b rpa1d double mutant plants display slow growth and developmental defects under non-damaging conditions.”
t0“Arabidopsis”
Claim · model-written summary
Defective DNA replication likely reduced cell division in rpa1b rpa1d plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpa1b rpa1d mutante0
- Outcome
- reduction in cell divisione0
- Qualifier
- defective DNA replicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these defects in the rpa1b rpa1d mutant are likely the result of defective DNA replication leading to reduction in cell division.”
t0“Arabidopsis”
The same questions in other papers
radiation · molecular: expression · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.