Abstract
UV and blue light are important regulators of plant gene expression and development. We investigated the signal transduction processes involved in the induction of chalcone synthase (CHS) and phenylalanine ammonia-lyase (PAL) gene expression by UV-B and UV-A/blue light in an Arabidopsis cell suspension culture. Experiments with electron transport inhibitors indicated that plasma membrane redox activity is involved in both signal transduction pathways. Calcium ionophore treatment stimulated expression of the TOUCH3 gene, and this induction was strongly antagonized by UV-A/blue and UV-B light, suggesting that both light qualities may promote calcium efflux from the cytosol. Consistent with this hypothesis, experiments with specific inhibitors indicated that UV-B and UV-A/blue light regulate calcium levels in a cytosolic pool in part via the action of specific Ca2+-ATPases. On the basis of these and previous findings, we propose that plasma membrane redox activity, initiated by photoreception, is coupled to the regulation of calcium release from an intracellular store, generating a calcium signal that is required to induce CHS expression.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Electron transport inhibitor experiments implicated plasma membrane redox activity in both light signaling pathways.
- Taxon
- Arabidopsis
Arabidopsise1 - Outcome
- both signal transduction pathwayse0
- Qualifier
- plasma membrane redox activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experiments with electron transport inhibitors indicated that plasma membrane redox activity is involved in both signal transduction pathways”
e1“Arabidopsis cell suspension culture”
Claim · model-written summary
Calcium ionophore stimulated TOUCH3 expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Calcium ionophore treatmente0
- Gene
- TOUCH3e0
- Outcome
- expression of the TOUCH3 genee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calcium ionophore treatment stimulated expression of the TOUCH3 gene”
e1“Arabidopsis cell suspension culture”
Claim · model-written summary
UV-A/blue light antagonized calcium ionophore induction of TOUCH3.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- UV-A/bluee0
- Gene
- TOUCH3e1
- Outcome
- expression of the TOUCH3 genee1
- Direction
- antagonizede0
- Comparator
- Calcium ionophore treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this induction was strongly antagonized by UV-A/blue and UV-B light”
e1“Calcium ionophore treatment stimulated expression of the TOUCH3 gene”
e2“Arabidopsis cell suspension culture”
Claim · model-written summary
UV-B light antagonized calcium ionophore induction of TOUCH3.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- UV-B lighte0
- Gene
- TOUCH3e1
- Outcome
- expression of the TOUCH3 genee1
- Direction
- antagonizede0
- Comparator
- Calcium ionophore treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this induction was strongly antagonized by UV-A/blue and UV-B light”
e1“Calcium ionophore treatment stimulated expression of the TOUCH3 gene”
e2“Arabidopsis cell suspension culture”
Claim · model-written summary
UV-B and UV-A/blue light regulate cytosolic calcium levels partly through Ca2+-ATPases.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- UV-B and UV-A/blue lighte0
- Gene
- Ca2+-ATPasese0
- Outcome
- calcium levels in a cytosolic poole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-B and UV-A/blue light regulate calcium levels in a cytosolic pool in part via the action of specific Ca2+-ATPases”
e1“Arabidopsis cell suspension culture”
Claim · model-written summary
The authors propose that membrane redox activity couples photoreception to calcium release required for CHS expression.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- CHSe0
- Outcome
- CHS expressione0
- Qualifier
- calcium release from an intracellular storee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plasma membrane redox activity, initiated by photoreception, is coupled to the regulation of calcium release from an intracellular store, generating a calcium signal that is required to induce CHS expression”
e1“Arabidopsis cell suspension culture”
The same questions in other papers
uv · molecular: expression · ArabidopsisPositive and negative results
6 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →light · molecular: expression · ArabidopsisPositive and negative results
24 papers in the same direction as this paper · 55 in the opposite direction
- positive
- positive
- positive
76 papers more in this group
See all 80 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.