Abstract
The C4 protein from Curtovirus is known as a major symptom determinant, but the mode of action of the C4 protein remains unclear. To understand the mechanism of involvement of C4 protein in virus-plant interactions, we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter; the resulting overexpression of BSCTV C4 led to abnormal host cell division. RKP, a RING finger protein, which is a homolog of the human cell cycle regulator KPC1, was discovered to be induced by BSCTV C4 protein. Mutation of RKP reduced the susceptibility to BSCTV in Arabidopsis and impaired BSCTV replication in plant cells. Callus formation is impaired in rkp mutants, indicating a role of RKP in the plant cell cycle. RKP was demonstrated to be a functional ubiquitin E3 ligase and is able to interact with cell-cycle inhibitor ICK/KRP proteins in vitro. Accumulation of the protein ICK2/KRP2 was found increased in the rkp mutant. The above results strengthen the possibility that RKP might regulate the degradation of ICK/KRP proteins. In addition, the protein level of ICK2/KRP2 was decreased upon BSCTV infection. Overexpression of ICK1/KRP1 in Arabidopsis could reduce the susceptibility to BSCTV. In conclusion, we found that RKP is induced by BSCTV C4 and may affect BSCTV infection by regulating the host cell cycle.
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Claims 10
Claim · model-written summary
Overexpression of BSCTV C4 in Arabidopsis led to abnormal host cell division.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of BSCTV C4e1
- Interactor
- Beet severe curly top virusBeet severe curly top viruse0pathogen
- Outcome
- host cell divisione1
- Qualifier
- abnormale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e1“the resulting overexpression of BSCTV C4 led to abnormal host cell division”
Claim · model-written summary
RKP was induced by the BSCTV C4 protein.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- Beet severe curly top virusBeet severe curly top viruse0pathogen
- Gene
- RKPe2
- Outcome
- was discovered to be inducede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e2“RKP, a RING finger protein, which is a homolog of the human cell cycle regulator KPC1, was discovered to be induced by BSCTV C4 protein”
Claim · model-written summary
Mutation of RKP reduced the susceptibility of Arabidopsis to BSCTV.
- Taxon
- ArabidopsisArabidopsise3
- Treatment
- Mutation of RKPe3
- Interactor
- BSCTVe3pathogen
- Gene
- RKPe3
- Outcome
- susceptibility to BSCTVe3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Mutation of RKP reduced the susceptibility to BSCTV in Arabidopsis”
Claim · model-written summary
Mutation of RKP impaired BSCTV replication in Arabidopsis cells.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutation of RKPe3
- Interactor
- BSCTVe4pathogen
- Outcome
- BSCTV replicatione4
- Direction
- impairede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e3“Mutation of RKP reduced the susceptibility to BSCTV in Arabidopsis”
e4“impaired BSCTV replication in plant cells”
Claim · model-written summary
Callus formation is impaired in rkp mutants, indicating a role of RKP in the plant cell cycle.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- rkp mutantse5
- Gene
- RKPe5
- Outcome
- Callus formatione5
- Direction
- impairede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e5“Callus formation is impaired in rkp mutants, indicating a role of RKP in the plant cell cycle”
Claim · model-written summary
RKP is a functional ubiquitin E3 ligase.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- RKPe6
- Outcome
- functional ubiquitin E3 ligasee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e6“RKP was demonstrated to be a functional ubiquitin E3 ligase”
Claim · model-written summary
RKP is able to interact with cell-cycle inhibitor ICK/KRP proteins in vitro.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ICK/KRPe7
- Outcome
- able to interact with cell-cycle inhibitor ICK/KRP proteinse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e7“is able to interact with cell-cycle inhibitor ICK/KRP proteins in vitro”
Claim · model-written summary
Accumulation of ICK2/KRP2 protein was increased in the rkp mutant.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- rkp mutante1
- Gene
- ICK2/KRP2e1
- Outcome
- Accumulation of the protein ICK2/KRP2e1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e1“Accumulation of the protein ICK2/KRP2 was found increased in the rkp mutant”
Claim · model-written summary
The ICK2/KRP2 protein level was decreased upon BSCTV infection.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- BSCTV infectione1
- Interactor
- BSCTVe1pathogen
- Gene
- ICK2/KRP2e1
- Outcome
- protein level of ICK2/KRP2e1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter”
e1“the protein level of ICK2/KRP2 was decreased upon BSCTV infection”
Claim · model-written summary
Overexpression of ICK1/KRP1 in Arabidopsis reduced the susceptibility to BSCTV.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Overexpression of ICK1/KRP1e1
- Interactor
- BSCTVe1pathogen
- Gene
- ICK1/KRP1e1
- Outcome
- susceptibility to BSCTVe1
- Direction
- reducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of ICK1/KRP1 in Arabidopsis could reduce the susceptibility to BSCTV”
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