Abstract
The Arabidopsis resistance protein HRT recognizes the Turnip crinkle virus (TCV) coat protein (CP) to induce a hypersensitive response (HR) in the resistant ecotype Di-17. The CP also interacts with a nuclear-targeted NAC family of host transcription factors, designated TIP (TCV-interacting protein). Because binding of CP to TIP prevents nuclear localization of TIP, it has been proposed that TIP serves as a guardee for HRT. Here, we have tested the requirement for TIP in HRT-mediated HR and resistance by analyzing plants carrying knockout mutation in the TIP gene. Our results show that loss of TIP does not alter HR or resistance to TCV. Furthermore, the mutation in TIP neither impaired the salicylic acid-mediated induction of HRT expression nor the enhanced resistance conferred by overexpression of HRT. Strikingly, the mutation in TIP resulted in increased replication of TCV and Cucumber mosaic virus, suggesting that TIP may play a role in basal resistance but is not required for HRT-mediated signaling.
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Claims 6
Claim · model-written summary
TIP loss did not alter the hypersensitive response to TCV.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- loss of TIPe0
- Interactor
- TCVe0pathogen
- Gene
- TIPe0
- Outcome
- HRe0
- Direction
- does not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of TIP does not alter HR or resistance to TCV”
t0“Arabidopsis”
Claim · model-written summary
TIP loss did not alter resistance to TCV.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- loss of TIPe0
- Interactor
- TCVe0pathogen
- Gene
- TIPe0
- Outcome
- resistance to TCVe0
- Direction
- does not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of TIP does not alter HR or resistance to TCV”
t0“Arabidopsis”
Claim · model-written summary
TIP mutation did not impair salicylic acid mediated HRT expression induction.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- the mutation in TIPe0
- Gene
- HRTe0
- Outcome
- salicylic acid-mediated induction of HRT expressione0
- Direction
- neither impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutation in TIP neither impaired the salicylic acid-mediated induction of HRT expression nor the enhanced resistance conferred by overexpression of HRT”
t0“Arabidopsis”
Claim · model-written summary
TIP mutation did not impair enhanced resistance conferred by HRT overexpression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- the mutation in TIPe0
- Interactor
- TCVe1pathogen
- Gene
- HRTe0
- Outcome
- enhanced resistancee0
- Direction
- neither impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutation in TIP neither impaired the salicylic acid-mediated induction of HRT expression nor the enhanced resistance conferred by overexpression of HRT”
t0“Turnip crinkle virus in Arabidopsis”
e1“loss of TIP does not alter HR or resistance to TCV”
Claim · model-written summary
TIP mutation increased TCV replication.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- the mutation in TIPe0
- Interactor
- TCVe0pathogen
- Gene
- TIPe0
- Outcome
- replication of TCVe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutation in TIP resulted in increased replication of TCV and Cucumber mosaic virus”
t0“Arabidopsis”
Claim · model-written summary
TIP mutation increased Cucumber mosaic virus replication.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- the mutation in TIPe0
- Interactor
- Cucumber mosaic virus
cucumber mosaic cucumoviruse0pathogen - Gene
- TIPe0
- Outcome
- replication of TCV and Cucumber mosaic viruse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutation in TIP resulted in increased replication of TCV and Cucumber mosaic virus”
t0“Arabidopsis”
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