Abstract
NADPH oxidase AtrbohD plays very important roles in modulating many cellular processes through production of signal molecules reactive oxygen species in Arabidopsis. However, whether it regulates the response to waterlogging stress is unclear. In this report, we showed that expression of AtrbohD was markedly induced by waterlogging stress, and mutation in AtrbohD led to clear sensitivity of Arabidopsis plants to waterlogging stress. Moreover, waterlogging-promoted increases in alcohol dehydrogenase (ADH) activity, ADH1 expression and H2O2 accumulation were significantly attenuated in two mutant lines of AtrbohD. These results indicate that AtrbohD is required for Arabidopsis tolerance to waterlogging stress. Besides, GUS staining experiments revealed that disruption of small G protein ROP2 encoding gene evidently suppressed the increase of AtrbohD expression while defect of AtrbohD did not prominently affect the abundance enhancements of ROP2 transcripts under waterlogged conditions. Together, these data suggest that AtrbohD functions downstream of ROP2 to positively regulate the response to waterlogging stress in Arabidopsis.
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Claims 8
Claim · model-written summary
Waterlogging markedly induced AtrbohD expression.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- waterlogging stresse0
- Gene
- AtrbohDe0
- Outcome
- expression of AtrbohDe0
- Direction
- markedly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of AtrbohD was markedly induced by waterlogging stress”
t0“Arabidopsis”
Claim · model-written summary
AtrbohD mutation increased plant sensitivity to waterlogging.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mutation in AtrbohDe0
- Gene
- AtrbohDe0
- Outcome
- sensitivity of Arabidopsis plants to waterlogging stresse0
- Direction
- clear sensitivitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation in AtrbohD led to clear sensitivity of Arabidopsis plants to waterlogging stress”
Claim · model-written summary
AtrbohD mutation attenuated the waterlogging increase in ADH activity.
- Taxon
- ArabidopsisArabidopsist0
- Material
- two mutant lines of AtrbohDe0
- Treatment
- waterlogginge0
- Outcome
- alcohol dehydrogenase (ADH) activitye0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“waterlogging-promoted increases in alcohol dehydrogenase (ADH) activity, ADH1 expression and H2O2 accumulation were significantly attenuated in two mutant lines of AtrbohD”
t0“Arabidopsis”
Claim · model-written summary
AtrbohD mutation attenuated waterlogging induced ADH1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Material
- two mutant lines of AtrbohDe0
- Treatment
- waterlogginge0
- Gene
- ADH1e0
- Outcome
- ADH1 expressione0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“waterlogging-promoted increases in alcohol dehydrogenase (ADH) activity, ADH1 expression and H2O2 accumulation were significantly attenuated in two mutant lines of AtrbohD”
t0“Arabidopsis”
Claim · model-written summary
AtrbohD mutation attenuated waterlogging induced H2O2 accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- two mutant lines of AtrbohDe0
- Treatment
- waterlogginge0
- Outcome
- H2O2 accumulatione0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“waterlogging-promoted increases in alcohol dehydrogenase (ADH) activity, ADH1 expression and H2O2 accumulation were significantly attenuated in two mutant lines of AtrbohD”
t0“Arabidopsis”
Claim · model-written summary
ROP2 disruption suppressed the increase of AtrbohD expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- disruption of small G protein ROP2 encoding genee0
- Gene
- AtrbohDe0
- Outcome
- increase of AtrbohD expressione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disruption of small G protein ROP2 encoding gene evidently suppressed the increase of AtrbohD expression”
t0“Arabidopsis”
Claim · model-written summary
AtrbohD defect did not prominently affect ROP2 transcript enhancement under waterlogging.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- defect of AtrbohDe0
- Gene
- ROP2e0
- Outcome
- abundance enhancements of ROP2 transcriptse0
- Direction
- did not prominently affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“defect of AtrbohD did not prominently affect the abundance enhancements of ROP2 transcripts under waterlogged conditions”
t0“Arabidopsis”
Claim · model-written summary
AtrbohD functions downstream of ROP2 in a positive waterlogging response.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtrbohDe0
- Outcome
- response to waterlogging stresse0
- Qualifier
- downstream of ROP2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtrbohD functions downstream of ROP2 to positively regulate the response to waterlogging stress in Arabidopsis”
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