Abstract
ABSTRACT In many plants, translocation of sucrose from mesnsophyll to phloem for long‐distance transport is carrier‐mediated. The sucrose H + ‐symporter gene SUT1 from potato is expressed at high levels in the phloem of mature, exporting leaves and at lower levels in other organs. Inhibition of SUT1 by expression of an antisense gene in companion cells under control of the rolC promoter leads to accumulation of high amounts of soluble and insoluble carbohydrates in leaves and inhibition of photosynthesis. The distribution of in situ localized starch does not correspond with areas of reduced photosynthesis as shown by fluorescence imaging. Dissection of antisense effects on sink and source organs by reciprocal grafts shows that inhibition of transporter gene expression in leaves is sufficient to produce chlorosis in leaves and reduced tuber yield. In contrast to the arrest of plasmodesmal development found in plants that express yeast invertase in the apoplast, in mature leaves of sucrose transporter antisense plants plasmodesmata are branched and have median cavities. These data strongly support an apoplastic mode of phloem loading in potato, in which the sucrose transporter located at the plasma membrane of the sieve element/companion cell complex represents the primary route for sugar uptake into the long‐distance translocation pathway.
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Claims 8
Claim · model-written summary
SUT1 expression is high in phloem of mature exporting leaves and lower in other organs.
- Taxon
- potato
Solanum tuberosume0 - Organ / stage
- phloem of mature, exporting leavese0
- Gene
- SUT1e0
- Outcome
- expressed at high levelse0
- Comparator
- other organse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sucrose H + ‐symporter gene SUT1 from potato is expressed at high levels in the phloem of mature, exporting leaves and at lower levels in other organs.”
Claim · model-written summary
Companion cell antisense inhibition of SUT1 accumulated carbohydrates in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Inhibition of SUT1 by expression of an antisense genee0
- Gene
- SUT1e0
- Outcome
- accumulation of high amounts of soluble and insoluble carbohydrates in leavese0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of SUT1 by expression of an antisense gene in companion cells under control of the rolC promoter leads to accumulation of high amounts of soluble and insoluble carbohydrates in leaves and inhibition of photosynthesis.”
t0“potato sucrose transporter SUT1”
Claim · model-written summary
Companion cell antisense inhibition of SUT1 inhibited photosynthesis.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Inhibition of SUT1 by expression of an antisense genee0
- Gene
- SUT1e0
- Outcome
- photosynthesise0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of SUT1 by expression of an antisense gene in companion cells under control of the rolC promoter leads to accumulation of high amounts of soluble and insoluble carbohydrates in leaves and inhibition of photosynthesis.”
t0“potato sucrose transporter SUT1”
Claim · model-written summary
Starch localization does not match areas with reduced photosynthesis.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- starche0
- Outcome
- areas of reduced photosynthesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The distribution of in situ localized starch does not correspond with areas of reduced photosynthesis as shown by fluorescence imaging.”
t0“potato sucrose transporter SUT1”
Claim · model-written summary
Leaf inhibition of transporter expression produced chlorosis.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inhibition of transporter gene expression in leavese0
- Outcome
- chlorosis in leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of transporter gene expression in leaves is sufficient to produce chlorosis in leaves and reduced tuber yield.”
t0“potato sucrose transporter SUT1”
Claim · model-written summary
Leaf inhibition of transporter expression reduced tuber yield.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inhibition of transporter gene expression in leavese0
- Outcome
- tuber yielde0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of transporter gene expression in leaves is sufficient to produce chlorosis in leaves and reduced tuber yield.”
t0“potato sucrose transporter SUT1”
Claim · model-written summary
Mature antisense leaves had branched plasmodesmata with median cavities.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- mature leavese0
- Outcome
- plasmodesmata are branched and have median cavitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in mature leaves of sucrose transporter antisense plants plasmodesmata are branched and have median cavities.”
t0“potato sucrose transporter SUT1”
Claim · model-written summary
The data support apoplastic phloem loading with SUT1 as primary sugar uptake route.
- Taxon
- potatoSolanum tuberosume0
- Gene
- sucrose transportere0
- Outcome
- apoplastic mode of phloem loadinge0
- Qualifier
- primary route for sugar uptake into the long‐distance translocation pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These data strongly support an apoplastic mode of phloem loading in potato, in which the sucrose transporter located at the plasma membrane of the sieve element/companion cell complex represents the primary route for sugar uptake into the long‐distance translocation pathway.”
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