Abstract
Vascular bundles regulate water balance, nutrient uptake and transport, and stress responses, ultimately influencing the yield and quality of crops. However, our understanding of the genetic functions of microRNAs (miRNAs) during vascular development remains limited. In this research, the role of miR169a in potatoes was studied. Silencing StmiR169a in potatoes promoted vascular bundle formation, resulting in not only upright and robust stems but also longer roots and more extensive root systems. Histological analysis revealed a significant increase in the number of xylem vessels in the vascular bundles of stems and roots of RNAi-mediated miR169a lines (STTM169). Silencing miR169a led to higher water use efficiency, enhanced photosynthesis rates, elevated enzymatic antioxidant activity, and reduced levels of reactive oxygen species (ROS), thereby enhancing the drought resistance of potatoes. However, overexpression of miR169a lines (OE169a) showed the opposite effects. The nuclear factor Y subunit NF-YA3 was identified as a target gene of StmiR169a. The miR169a/NF-YA3 module may be involved in the regulation of potato vascular bundle development and the response to drought stress.
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Claims 10
Claim · model-written summary
Silencing StmiR169a promoted vascular bundle formation in potato.
- Taxon
- Solanum tuberosum
Solanum tuberosumt0 - Treatment
- Silencing StmiR169ae1
- Gene
- StmiR169ae1
- Outcome
- vascular bundle formatione1
- Direction
- promotede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e1“Silencing StmiR169a in potatoes promoted vascular bundle formation, resulting in not only upright and robust stems but also longer roots and more extensive root systems”
Claim · model-written summary
Silencing StmiR169a led to longer roots and more extensive root systems in potato.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Treatment
- Silencing StmiR169ae1
- Outcome
- root systemse1
- Direction
- more extensivee1
- Qualifier
- longer rootse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e1“Silencing StmiR169a in potatoes promoted vascular bundle formation, resulting in not only upright and robust stems but also longer roots and more extensive root systems”
Claim · model-written summary
Silencing miR169a increased the number of xylem vessels in the vascular bundles of stems and roots.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Organ / stage
- vascular bundles of stems and rootse2
- Treatment
- RNAi-mediated miR169a lines (STTM169)e2
- Outcome
- number of xylem vesselse2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e2“Histological analysis revealed a significant increase in the number of xylem vessels in the vascular bundles of stems and roots of RNAi-mediated miR169a lines (STTM169)”
Claim · model-written summary
Silencing miR169a led to higher water use efficiency in potato.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Treatment
- Silencing miR169ae3
- Outcome
- water use efficiencye3
- Direction
- highere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e3“Silencing miR169a led to higher water use efficiency, enhanced photosynthesis rates, elevated enzymatic antioxidant activity, and reduced levels of reactive oxygen species (ROS), thereby enhancing the drought resistance of potatoes”
Claim · model-written summary
Silencing miR169a enhanced photosynthesis rates in potato.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Treatment
- Silencing miR169ae3
- Outcome
- photosynthesis ratese3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e3“Silencing miR169a led to higher water use efficiency, enhanced photosynthesis rates, elevated enzymatic antioxidant activity, and reduced levels of reactive oxygen species (ROS), thereby enhancing the drought resistance of potatoes”
Claim · model-written summary
Silencing miR169a elevated enzymatic antioxidant activity in potato.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Treatment
- Silencing miR169ae3
- Outcome
- enzymatic antioxidant activitye3
- Direction
- elevatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e3“Silencing miR169a led to higher water use efficiency, enhanced photosynthesis rates, elevated enzymatic antioxidant activity, and reduced levels of reactive oxygen species (ROS), thereby enhancing the drought resistance of potatoes”
Claim · model-written summary
Silencing miR169a reduced levels of reactive oxygen species in potato.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Treatment
- Silencing miR169ae3
- Outcome
- levels of reactive oxygen species (ROS)e3
- Direction
- reducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e3“Silencing miR169a led to higher water use efficiency, enhanced photosynthesis rates, elevated enzymatic antioxidant activity, and reduced levels of reactive oxygen species (ROS), thereby enhancing the drought resistance of potatoes”
Claim · model-written summary
Overexpression of miR169a showed the opposite of the silencing effects in potato.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Treatment
- overexpression of miR169a lines (OE169a)e4
- Outcome
- effectse4
- Direction
- oppositee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Schema note from the model: outcome not named in the abstract; opposite of the silencing effects
Verbatim
t0“Solanum tuberosum L”
e4“However, overexpression of miR169a lines (OE169a) showed the opposite effects”
Claim · model-written summary
NF-YA3 was identified as a target gene of StmiR169a.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Gene
- NF-YA3e5
- Outcome
- target genee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e5“The nuclear factor Y subunit NF-YA3 was identified as a target gene of StmiR169a”
Claim · model-written summary
The miR169a/NF-YA3 module may be involved in regulating potato vascular bundle development and the response to drought.
- Taxon
- Solanum tuberosumSolanum tuberosumt0
- Gene
- miR169a/NF-YA3 modulee6
- Outcome
- vascular bundle developmente6
- Qualifier
- may be involvede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Solanum tuberosum L”
e6“The miR169a/NF-YA3 module may be involved in the regulation of potato vascular bundle development and the response to drought stress”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.