Abstract
Water limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloroplast electron distribution by introducing alternative electron transport sinks has been shown to increase plant tolerance to multiple environmental challenges including hydric stress, suggesting that a similar strategy could be used to improve drought tolerance in crops. We show herein that the expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought. Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance. Interestingly, 5-6% of leaf-expressed genes were affected by flavodoxin in the absence of drought, representing pathways modulated by chloroplast redox status during normal growth. About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants. Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants, indicating that the flavoprotein has the potential to improve major agronomic traits in potato.
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Claims 6
Claim · model-written summary
Flavodoxin expression protected photosynthetic activities at a pre-symptomatic drought stage.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- expression of the cyanobacterial electron shuttle flavodoxine0
- Gene
- flavodoxine0
- Outcome
- photosynthetic activitiese0
- Direction
- protectede0
- Qualifier
- pre-symptomatic stage of droughte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought”
Claim · model-written summary
Flavodoxin expressing plants had an attenuated transcriptional and metabolic drought response.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Treatment
- flavodoxin-expressinge0
- Gene
- flavodoxine0
- Outcome
- response to the adverse conditione0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants”
t0“Potato Plants”
Claim · model-written summary
The attenuated response correlated with increased stress tolerance.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Treatment
- flavodoxin-expressinge0
- Outcome
- stress tolerancee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance”
t0“Potato Plants”
Claim · model-written summary
Flavodoxin affected 5-6% of leaf-expressed genes without drought.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Treatment
- flavodoxine0
- Outcome
- leaf-expressed genese0
- Value
- 5-6%e0
- Qualifier
- in the absence of droughte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5-6% of leaf-expressed genes were affected by flavodoxin in the absence of drought”
t0“Potato Plants”
Claim · model-written summary
About 300 flavodoxin-modulated genes changed in the same direction as wild-type drought responses and potentially contribute to acclimation.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Gene
- flavodoxine0
- Outcome
- genes potentially contribute to stress acclimatione0
- Value
- About 300e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants”
t0“Potato Plants”
Claim · model-written summary
Flavodoxin expression mitigated tuber yield losses under chronic water limitation.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Treatment
- flavodoxin-expressinge0
- Outcome
- Tuber yield lossese0
- Direction
- mitigatede0
- Qualifier
- chronic water limitatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants”
t0“Potato Plants”
The same questions in other papers
transgenic · physiology: photosynthesis · Solanum tuberosum (potato)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
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