Abstract
To slow down reducing sugar accumulation of potato tubers exposed to low temperature storage, an expression vector pBICNI of Nt-VIF gene, a vacuolar invertase inhibitor from Nicotiana tabacum, regulated by the potato tuber specific promoter CIPP was contructed and transformed into potato(Solanum tuberosum) cv. E-potato 3 (E3). Detections by the PCR, Northern and Southern hybridizations indicated that the full longth Nt-VIF Nt-VIF cDNA was successfully transformed into cv. E3. After storing potato tubers of 14 transgenic lines in 4 ℃ or 20 ℃ for 30 d, the activities of vaculoar invertase (VI) and reducing sugar(RS) content were analysed. The results showed that there were no significant differences in RS content between transgenic and untransformed(control) tubers stored in 20℃. However, compared to the control, RS content of transgenic lines reduced obviously in 4 ℃ from 34.0% (line B-13) to 76.8% (line B-1), implying inhibition of VI activity by Nt-VIF cDNA expression resulted in RS content reduction. Further analysis revealed a positive linear relationship between IV activity and RS content (VI = 0.308RS + 0.067). Lines B-1, B-2, B-6, B-9 and B-14 can meet the requirements of potato fries in terms of their low RS content after the cold-storage.
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Claims 6
Claim · model-written summary
Nt-VIF cDNA was successfully transformed into potato cultivar E3.
- Taxon
- potato
Solanum tuberosume1 - Material
- cv. E3e0
- Gene
- Nt-VIFe0
- Outcome
- successfully transformede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the full longth Nt-VIF Nt-VIF cDNA was successfully transformed into cv. E3”
e1“potato(Solanum tuberosum) cv. E-potato 3 (E3)”
Claim · model-written summary
At 20℃, transgenic and untransformed potato tubers did not differ significantly in reducing sugar content.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- transgenice0
- Outcome
- RS contente0
- Direction
- no significant differencese0
- Qualifier
- stored in 20℃e0
- Comparator
- untransformed(control)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were no significant differences in RS content between transgenic and untransformed(control) tubers stored in 20℃”
e1“potato tubers of 14 transgenic lines”
Claim · model-written summary
At 4 ℃, transgenic potato tubers had 34.0% to 76.8% lower reducing sugar than controls.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- transgenic linese0
- Outcome
- RS contente0
- Direction
- reducede0
- Value
- 34.0%e0
- Qualifier
- 4 ℃e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared to the control, RS content of transgenic lines reduced obviously in 4 ℃ from 34.0% (line B-13) to 76.8% (line B-1)”
e1“potato tubers of 14 transgenic lines”
Claim · model-written summary
Line B-1 reduced reducing sugar by 76.8% against controls at 4 ℃.
- Taxon
- potatoSolanum tuberosume1
- Material
- line B-1e0
- Treatment
- transgenic linese0
- Outcome
- RS contente0
- Direction
- reducede0
- Value
- 76.8%e0
- Qualifier
- 4 ℃e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared to the control, RS content of transgenic lines reduced obviously in 4 ℃ from 34.0% (line B-13) to 76.8% (line B-1)”
e1“potato tubers of 14 transgenic lines”
Claim · model-written summary
Invertase activity and reducing sugar content had a positive linear relationship.
- Taxon
- potatoSolanum tuberosume1
- Gene
- IVe0
- Outcome
- RS contente0
- Direction
- positivee0
- Value
- VI = 0.308RS + 0.067e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a positive linear relationship between IV activity and RS content (VI = 0.308RS + 0.067)”
e1“potato tubers of 14 transgenic lines”
Claim · model-written summary
Lines B-1, B-2, B-6, B-9 and B-14 met potato fry requirements after cold storage because of low reducing sugar.
- Taxon
- potatoSolanum tuberosume1
- Material
- Lines B-1, B-2, B-6, B-9 and B-14e0
- Outcome
- meet the requirements of potato friese0
- Qualifier
- low RS content after the cold-storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lines B-1, B-2, B-6, B-9 and B-14 can meet the requirements of potato fries in terms of their low RS content after the cold-storage.”
e1“potato tubers of 14 transgenic lines”
The same questions in other papers
transgenic · quality: sugar starch · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- no effect
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.