Abstract
Pod storage protein (PSP) accumulates in both developing pods and wounded leaves of common bean (Phaseolus vulgaris). Since jasmonic acid (JA) is known to be involved in wound responses, we examined hormonal regulation of PSP expression. Methyl jasmonate (MeJA)-induced expression of PSP was blocked by both salicylic acid (SA) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC). The PSP promoter fused to β-glucuronidase (GUS) coding region was introduced into Arabidoposis thaliana using Agrobacterium infection. No MeJA-inducible GUS expression was found in the transformants. The chimeric gene was then introduced into stems of common bean using particle bombardment. Exogenously applied MeJA enhanced GUS activity, and this was prevented by both SA and ACC. Deletion analysis of the promoter indicated that the region between positions-747 and-555 included cis-regulatory elements for JA induction and ethylene suppression, and that cis-elements for SA-suppression were located in the region downstream of position-86.
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Claims 9
Claim · model-written summary
PSP accumulates in developing pods and wounded leaves of common bean.
- Taxon
- common bean
Phaseolus vulgarise0 - Organ / stage
- developing pods and wounded leavese0
- Gene
- Pod storage protein (PSP)e0
- Outcome
- accumulatese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pod storage protein (PSP) accumulates in both developing pods and wounded leaves of common bean (Phaseolus vulgaris)”
Claim · model-written summary
Salicylic acid blocked methyl jasmonate-induced PSP expression in common bean.
- Taxon
- Common BeanPhaseolus vulgarist0
- Treatment
- salicylic acid (SA)e0
- Gene
- PSPe0
- Outcome
- expression of PSPe0
- Direction
- blockede0
- Comparator
- Methyl jasmonate (MeJA)-inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“Methyl jasmonate (MeJA)-induced expression of PSP was blocked by both salicylic acid (SA) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC)”
Claim · model-written summary
The ethylene precursor ACC blocked methyl jasmonate-induced PSP expression in common bean.
- Taxon
- Common BeanPhaseolus vulgarist0
- Treatment
- 1-aminocyclopropane-1-carboxylic acid (ACC)e0
- Gene
- PSPe0
- Outcome
- expression of PSPe0
- Direction
- blockede0
- Comparator
- Methyl jasmonate (MeJA)-inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“Methyl jasmonate (MeJA)-induced expression of PSP was blocked by both salicylic acid (SA) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC)”
Claim · model-written summary
Arabidoposis thaliana transformants carrying the PSP promoter-GUS fusion had no MeJA-inducible GUS expression.
Not reportedDirection
Verbatim
e0“The PSP promoter fused to β-glucuronidase (GUS) coding region was introduced into Arabidoposis thaliana using Agrobacterium infection. No MeJA-inducible GUS expression was found in the transformants”
Claim · model-written summary
Exogenous MeJA enhanced GUS activity in bombarded common bean stems.
- Taxon
- common beanPhaseolus vulgarise0
- Organ / stage
- stemse0
- Treatment
- Exogenously applied MeJAe0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“The chimeric gene was then introduced into stems of common bean using particle bombardment. Exogenously applied MeJA enhanced GUS activity”
Claim · model-written summary
SA prevented the MeJA enhancement of GUS activity in common bean stems.
- Taxon
- Common BeanPhaseolus vulgarist0
- Treatment
- SAe0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- preventede0
- Comparator
- MeJAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“Exogenously applied MeJA enhanced GUS activity, and this was prevented by both SA and ACC”
Claim · model-written summary
ACC prevented the MeJA enhancement of GUS activity in common bean stems.
- Taxon
- Common BeanPhaseolus vulgarist0
- Treatment
- ACCe0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- preventede0
- Comparator
- MeJAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“Exogenously applied MeJA enhanced GUS activity, and this was prevented by both SA and ACC”
Claim · model-written summary
The PSP promoter region between positions -747 and -555 contains elements for JA induction and ethylene suppression.
- Taxon
- Common BeanPhaseolus vulgarist0
- Gene
- cis-regulatory elementse0
- Outcome
- JA induction and ethylene suppressione0
- Qualifier
- region between positions-747 and-555e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“the region between positions-747 and-555 included cis-regulatory elements for JA induction and ethylene suppression”
Claim · model-written summary
The PSP promoter region downstream of position -86 contains elements for SA suppression.
- Taxon
- Common BeanPhaseolus vulgarist0
- Gene
- cis-elementse0
- Outcome
- SA-suppressione0
- Qualifier
- region downstream of position-86e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Common Bean Plants”
e0“cis-elements for SA-suppression were located in the region downstream of position-86”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →salicylic acid · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →salicylic acid · quality: protein · Phaseolus vulgaris (common bean)Positive and negative results
8 in the opposite direction
- positive
- positive
- positive
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.