Paper · 2000 · Xibei zhiwu xuebao

Expression analysis of DnaJ-like protein gene from bean under various environment stresses

Yu Zhang

9
claims
16
verbatim quotes
2
citations
3
research areas
—
Not open access

Abstract

PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library.Northern blot analysis revealed that the transcripts of PvSR6 were at low levels in unstressed bean leaves,but the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid.These suggested that PvSR6 might have important roles in resistance to heavy metals and various environmental stresses by maintaining the structure and function of membranes and enzymes.

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Claims 9

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A PvSR6 clone encoding a DnaJ-like protein was isolated from a treated bean cDNA library.

Taxon
beane0
Gene
PvSR6e0
Outcome
clone encoding a new member of the DnaJ like protein familye0

Not reportedValue

Verbatim

e0

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PvSR6 transcript levels were low in unstressed bean leaves.

Taxon
beane0
Organ / stage
bean leavese0
Gene
PvSR6e0
Outcome
transcripts of PvSR6e0
Direction
low levelse0

Verbatim

e0

“the transcripts of PvSR6 were at low levels in unstressed bean leaves”

✓ located verbatim in the source · abstract, characters 241–310 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PvSR6 expression was stimulated by Hg 2+.

Taxon
beane1
Treatment
Hg 2+e0
Gene
PvSR6e1
Outcome
genes expressione0
Direction
strongly stimulatede0

Not reportedComparator

Verbatim

e0

“the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid”

✓ located verbatim in the source · abstract, characters 315–467 · show in text ↑
e1

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PvSR6 expression was stimulated by Cd 2+.

Taxon
beane1
Treatment
Cd 2+e0
Gene
PvSR6e1
Outcome
genes expressione0
Direction
strongly stimulatede0

Not reportedComparator

Verbatim

e0

“the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid”

✓ located verbatim in the source · abstract, characters 315–467 · show in text ↑
e1

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PvSR6 expression was stimulated by wounding.

Taxon
beane1
Treatment
woundinge0
Gene
PvSR6e1
Outcome
genes expressione0
Direction
strongly stimulatede0

Not reportedComparator

Verbatim

e0

“the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid”

✓ located verbatim in the source · abstract, characters 315–467 · show in text ↑
e1

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PvSR6 expression was stimulated by elevated temperature.

Taxon
beane1
Treatment
elevated temperaturee0
Gene
PvSR6e1
Outcome
genes expressione0
Direction
strongly stimulatede0

Not reportedComparator

Verbatim

e0

“the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid”

✓ located verbatim in the source · abstract, characters 315–467 · show in text ↑
e1

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PvSR6 expression was stimulated by ultraviolet irradiation.

Taxon
beane1
Treatment
ultraviolet irradiatione0
Gene
PvSR6e1
Outcome
genes expressione0
Direction
strongly stimulatede0

Not reportedComparator

Verbatim

e0

“the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid”

✓ located verbatim in the source · abstract, characters 315–467 · show in text ↑
e1

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PvSR6 expression was stimulated by salicylic acid.

Taxon
beane1
Treatment
salicylic acide0
Gene
PvSR6e1
Outcome
genes expressione0
Direction
strongly stimulatede0

Not reportedComparator

Verbatim

e0

“the genes expression was strongly stimulated by heavy metals (Hg 2+ and Cd 2+ ),wounding,elevated temperature,ultraviolet irradiation and salicylic acid”

✓ located verbatim in the source · abstract, characters 315–467 · show in text ↑
e1

“PvSR6 ( Phaseolus vulgaris stress related) clone encoding a new member of the DnaJ like protein family has been isolated from a mercuric chloride treated bean ( Phaseolus vulgaris L.cv.Saxa) cDNA library”

✓ located verbatim in the source · abstract, characters 0–203 · show in text ↑
Mechanismhypothesisoriginal resultpositivebasis n/a

Claim · model-written summary

The authors propose that PvSR6 supports stress resistance through membrane and enzyme maintenance.

Taxon
beant0
Gene
PvSR6e0
Outcome
resistance to heavy metals and various environmental stressese0
Qualifier
maintaining the structure and function of membranes and enzymese0

Verbatim

e0

“PvSR6 might have important roles in resistance to heavy metals and various environmental stresses by maintaining the structure and function of membranes and enzymes”

✓ located verbatim in the source · abstract, characters 489–653 · show in text ↑
t0

“bean”

✓ located verbatim in the source · title, characters 51–55 · show in text ↑

salicylic acid · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results

5 papers: 3 positive, 2 negative or no effect

2 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

1 paper more in this group

See all 5 papers →

heat · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

mechanical · molecular: expression · Phaseolus vulgaris (common bean)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

3 in the opposite direction

  1. no effect
  2. no effect
  3. no effect
See all 4 papers →

uv · molecular: expression · Phaseolus vulgaris (common bean)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.